We narrowed to 146 results for: Endog

...sample where your secondary antibody recognizes endogenous antigens. In these cases, even though one full...purposes, since the fragment could bind not only the endogenous proteins you are trying to block but your primary...

...our Hot Plasmids Podcast! TRIM-Away: Targeted endogenous protein degradation Article contributed by Mary... hr of treatment. In some primary cell lines, endogenous TRIM21 is sufficient for TRIM-Away, and you only...

... the bait. Also, RaPID can’t be used to study endogenous RNAs at their physiological expression levels...levels; however, knock-in of BoxB sites into endogenous DNA sequences could allow one to study RNAs expressed...

...pegRNAs (but not epegRNAs) can improve either endogenous or fused La binding (Yan et al., 2024). ...Adamson, B. (2024). Improving prime editing with an endogenous small RNA-binding protein. Nature, 628(8008),...

...signaling can also modify other properties such as endogenous gene expression, which can lead to changes in...with the goal of creating receptors with less endogenous activation and reduced off target activation ...

...heterotrimers with endogenous Gβγ, enabling detection of activation triggered by endogenous GPCR. Example ...

...Cas9, empty gRNA backbones, and constructs for endogenous tagging. Some examples of useful worm CRISPR ...Cas9 Editing Efficiency Fluorescent Tagging of Endogenous Genes with SapTrap Even more elegant: Single ...

...methods are not necessarily suitable for combining endogenous sequences. Table 2: High Throughput Cloning Strategies...modules themselves (so may not be suitable for endogenous coding sequences), so enzyme choice is critical...

...conjugated to molecular species with high affinity for endogenous molecular targets such as paclitaxel. Unlike ...required as the small-molecule binding motif targets endogenous protein (Figure 1f). Labeling strategy pros...

...typically expressed at much higher levels than endogenous proteins. Data obtained from experiments using...tagged protein may not be translatable to the endogenous system and will need to be verified using alternative...

...abasic (empty) DNA site using either fused or endogenous Uracil DNA N-glycosylase (UNG) enzymes. Various...efficiency, but it didn’t help: it seemed that the endogenous enzyme is not efficient enough to hinder adenine...

...the target antigen. Some examples could be an endogenous sample of wild type cells, purified protein, ...be sure to include a sample that expresses the endogenous protein, since folding of the recombinant protein...

...which are cleaved out of the RNA transcript by endogenous processing enzymes (Figure 1). To ensure this...et al. (2024) Improving prime editing with an endogenous small RNA-binding protein. Nature. 2024 Apr 3...

...from one of the transgene’s endogenous introns or comprised of non-endogenous sequences, such as a 77bp ...

...to a histone tail to recruit and stimulate the endogenous methyltransferase. The strategy is generalizable... protein (dCas9 shown here). D3L recruits the endogenous, autoinhibited methyltransferase DNMT3A to the...

...Tailored mutations are commonly introduced to endogeneous DNA through homology-directed repair (HDR) of...editing requires a template of high homology to the endogenous target, yet to facilitate the repair, the template...

... Targeted DNA demethylation and activation of endogenous genes using programmable TALE-TET1 fusion proteins...-specific editing of histone modifications at endogenous enhancers. Nature Biotechnology 31:1133–1136 ...

...albumin. Secondary antibodies can also react with endogenous antibodies naturally present in the sample, for...experimental run (Burry 2011). Autofluorescence Endogenous fluorescence of the sample, or autofluorescence...

...CNS myelination. (Lam et al. Nat Commun. 2022) Endogenously tag gene variants for Alzheimer's disease and...and Chemogenetics Plasmids . Find plasmids for endogenous gene tagging in human cells from the Allen Institute...

...that tests enhancer sequences outside of their endogenous chromatin environment and relies on the fact ...because it was difficult to fuse the AID tag to endogenous proteins. As described in their recent Cell Reports...genes of interest to generate mAID fusions to endogenous genes. Cell lines with the appropriate fusions...