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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 270 results for: FAS

Showing: 41 - 60 of 270 results

  1. TALEN Guide

    Type
    Collection
    ...assemble “multiple DNA fragments in an ordered fashion in a single reaction.” Using these plasmids, which...

  2. TALEN Plasmids and Kits

    Type
    Collection
    ...effector repeat arrays (assembled using the REAL, REAL-Fast, or FLASH methods) for expression as TALE-LSD1 fusions...

  3. Lentivirus Plasmids

    Type
    Collection
    ...plasmids 36083 and 36086 for other FP variants. Tsoulfas 41393 pCW57.1 3rd Inducible lentiviral expression...

  4. Deisseroth INTRSECT Collection

    Type
    Collection
    ...Cortical-Thalamic-Striatal Circuits through the Parafascicular Nucleus. Neuron 102(3):636-652. PubMed (Link...

  5. Plasmids for Stem Cell Research

    Type
    Collection
    .... This method of cell lineage conversion can be faster, bypasses intermediate pluripotent stages, and ...

  8. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

    Type
    Blog Post
    ...The idea was to evolve a TadA-7.10 version with a faster deamination rate so that deamination can happen...PANCE) selection systems to engineer better and faster ABEs (Fig. 2). These systems allow for rapid continuous...speed dictates which phage variants can propagate faster than the rate of dilution (passage from the infected...Created with BioRender.com. Introducing ABE8e - a fast and flexible adenine base editor Lower-stringency..., they identified TadA-8e, which edits 590-fold faster than TadA-7.10. It is compatible with all Cas9 ...resulting base editor was named ABE8e. ABE8e is not only fast, it can also be multiplexed: when tasked to correct...functional domains speed up the development towards faster and more precise base editors capable of editing...

  9. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    ...check out our blog post on choosing a Cas9 enzyme. Fast facts – PAM requirement: NGG and an expanding number...has anti-viral application potential as a result. Fast facts – PAM requirement: AAG or TTC  Best for: very...optimize it for therapeutic and laboratory usage. Fast facts – PAM requirement: TTN or TTTN. Best for:...variants such as single nucleotide polymorphisms. Fast facts – PAM requirement: none. Best for: high fidelity...information on Cas13, check out our blog on RNA editing. Fast facts – PAM requirement: protospacer flanking sequence...11 in our blog post on its development and use. Fast facts – PAM requirement: protospacer flanking sequence...targeting single stranded RNA.   Table 1: Fast facts on Cas family proteins and genome editing ...

  10. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ... below use the following optogenetic modules: VVDfast homodimerizes under blue light iLID-SspB heterodimerizes...limitations (Figure 1). Figure 1: Opto-katanin induces fast, localized microtubule severing. A) Schematic of...of Opto-katanin constructs and activity. EB3N-VVDfast-iLID (anchor) and SspB-mCherry-p60 (katanin) are... of two separate blue light-sensitive systems, VVDfast to control recruitment of an “anchor” to microtubules...anchor. So, you’ll need to express both the EB3N-VVDfast-iLID anchor module and the SspB-mCherry-p60 katanin...controlling cell dynamics. Need a refresher on VVDfast, iLID, LOV, CRY2-CIB, and other optogenetic modules...

  11. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    ...Verkhusha Multiple FAST Fluorescence-activating and absorption shifting tag (FAST) for use with green-yellow...superpositive GFP Split-superpositive GFP reassembly is a fast, efficient, and robust method for detecting protein-protein...mScarlet Split-wrmScarlet and split-sfGFP: tools for faster, easier fluorescent labeling of endogenous proteins...requires some time for chromophore maturation, splitFAST displays rapid and reversible complementation....

  12. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    ... 6.8 (red) 2.8/4.1 7.1 h (red) pFast-FT-N1 pTRE-Fast-FTpBAD/HisB-Fast-FT Medium-FT 401 (blue), 579...produced the three separate timers shown in the table: Fast-FT, Medium-FT, and Slow-FT. These display progressively...Emission (nm) Brightness pKa Maturation Plasmids Fast-FT 403 (blue), 583 (red) 466 (blue), 606 (red) ...

  13. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...Self-labeling tags for single-molecule biophysics Fast and responsive voltage indicator ONE-GO biosensors... https://doi.org/10.1101/2024.12.20.629623.  A fast and responsive voltage indicator with enhanced sensitivity...Lab and collaborators recently created ASAP5, a faster and more responsive genetically-encoded voltage...can be used in different illumination regimes with fast activation kinetics and high responsivity near the...Z., Jiang, D., Zhang, G., … Lin, M. Z. (2024). A fast and responsive voltage indicator with enhanced sensitivity...

  14. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ...affinity for glutamate but slower kinetics; v857 has faster kinetics but lower affinity) and with a variety...two-photon microscopy. The resulting JEDI-2P has faster response kinetics and increased brightness, sensitivity... Sustained deep-tissue voltage recording using a fast indicator evolved for two-photon microscopy. Cell...transfer at the subcellular level requires a probe with fast kinetics and high signal/noise ratio. Here, Takeharu...Figure 7: B-gTEMP temperature-sensitive probe enables fast measurement of temperature and local heat transfer...

  15. Which Fluorescence Microscopy Techniques is Best for Me?

    Type
    Blog Post
    ...captured in a single camera exposure. This is much faster than relying on raster scanning as is done in confocal...characteristic to capture in these types of samples is their fast movements. Depending on the sample, a temporal resolution...sample is then collected and projected back to a fast, sensitive detector such as a scientific CMOS camera...planes of a sample may also be useful for providing fast imaging along with optical sectioning (5). One such...preferred technique for many of these samples due to its fast temporal resolution and decreased phototoxicity....

  16. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type
    Blog Post
    ...increasingly faster kinetics. The opsin ChETA, for example, is a ChR2 opsin mutated to have faster off kinetics...kinetics and allow neurons to repolarize faster.  Chemogenetics does not offer the same level of control...design.   Optogenetics Chemogenetics Timing Fast activation/deactivation Prolonged activation Targeting...

  17. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    ...that I'm glad we're so fast and affordable. Something in their world should be fast and affordable. 4. Stem...after the OSKM paper – as a prime example of the fast movement in this field.  As an Addgene scientist...engineering technologies, stem cell techniques are neither fast nor inexpensive.  After talking to numerous poster...

  18. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...This post was contributed by Jae Lee and Pantelis Tsoulfas of the Department of Neurological Surgery at the...you to our guest bloggers, Jae Lee and Pantelis Tsoulfas. Jae K. Lee is an assistant Professor at the University... Find plasmids from the Lee Lab here. Pantelis Tsoulfas is an Associate Professor at the University of...Miller School of Medicine. Find plasmids from the Tsoulfas lab here.     References: H. Siedentopf, and ...

  19. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    ...Aligning two nucleotide sequences is probably the fastest BLAST search to perform and will save you time ...Highly similar sequences (megablast)”, which is very fast and works best when the identity between your sequence... [Our QC process would be trouble-free and much faster if 95% of our results were always correct!]  Since...base pair from your results. This option is not as fast as megablast, but can return longer alignments to...

  20. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    ... The pEcCas plasmid can be operated at 37 ℃ for faster growth of its E. coli host, and the addition of...industrial strain. Not only is MUCICAT better, it is faster. Using MUCICAT only needs 2 newly constructed plasmids...the MUCICAT system has the following advantages: Fast and site-specific multicopy chromosomal integration...on developing microbial genome editing tools for faster construction or reprogramming of cells to understand...
Showing: 41 - 60 of 270 results