We narrowed to 342 results for: GAI
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TypeBlog Post... fraction of readers cover the same ground again and again as they don’t save the insights they glean ...years of a project. Dissemination of the knowledge gained during this period occurs infrequently and rarely...researchers, we wondered how we could make the insights gained during this time more visible and thereby have ...
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The Strength of Story Telling
TypeBlog Post...is to keep working on telling a story again and again and again until you work out what people enjoy and...lessons you’ve learned and what knowledge you’ve gained. Show that as the story has progressed, you or ... -
Antibodies 101: Secondary Antibodies
TypeBlog Post... Secondary antibodies are antibodies generated against antibody isotypes from a specific species, such...possible to get secondary antibodies generated against isotype subclasses, such as mouse IgG2 or IgG4....unbound or excess antibody. Incubate the sample again with a conjugated secondary antibody, which will...anti-rabbit?!”* *a secondary antibody generated in goat against rabbit immunoglobulins. References and Resources... -
Antibodies 101: Flow Compensation
TypeBlog Post...and how do I fix it? When plotting two channels against each other, you should be able to draw perpendicular...single-color APC control, with APC and APC-CY7 plotted against each other. Since it is a single-color control,...After increasing the compensation values for APC against APC-Cy7, the populations are in more of a straight...compensation value of five was applied to APC-Cy7 against APC to correct for this bleed. A compensation value... -
Finding Your Perfect Job After University
TypeBlog Post...Research in Sutton, located on the edge of London to gain relevant experience. I applied for this summer position... I needed more practical lab experience to later gain a full time position in a lab. I worked on the genetics...Working in industry: virology On completing my MSc, I again had to look at what type of work I would like to...entitled to a Work-Travel VISA, which means I can gain employment for up to 6 months in Australia or another... -
Antibodies 101: Introduction to Antibodies
TypeBlog Post...animal, and its B cells will churn out antibodies against the injected protein. Several weeks later, blood...protein. However, each animal will make antibodies against different protein epitopes and so variability between... protein compared to whole antibodies (Crivianu-Gaita 2016). Get more details on ScFvs in this article...//doi.org/10.1016/j.ymeth.2017.01.009 Crivianu-Gaita V, Thompson M (2016) Aptamers, antibody scFv, and... -
Cancer and the Immune System: Deciphering the Relationship
TypeBlog Post...bodies’ natural abilities, we’re gaining new ground in the fight against cancer in its many forms. The ...sequence of immune reactions that aid in the fight against cancer. Sipuleucel-T by the Dendreon Corporation...will more broadly activate the immune response against tumors. Some chemotherapeutic agents induce immunogenic... -
With an Eye Towards the Future, We Look Back at the March for Science
TypeBlog Post...was a great, positive event that I hope happens again. Nicholas Thompson is a PhD Candidate studying Nuclear...cause breast cancer and infertility or to argue against unnecessary clinic regulations, evidence-based .... I worried that, because we were speaking out against legislators and because the march had been so well-publicized...marches across the world have had an impact already, gaining additional funding and the eyes of the world. Justin... -
MassBioEd Career Exploration Day at Addgene
TypeBlog Post...many different career options in science, with Abigail Anderson commenting that “There is not just a scientist...that means it is possible to switch your field.” Abigail was also impressed with the “comfortable surroundings...surroundings” of the new Addgene office space. Thanks Abigail, we think it’s pretty great, too! Thanks to MassBioEd... -
In Living Color: The Skinny on In Vivo Imaging Tools
TypeBlog Post...notice the name Vladislav Verkhusha popping up again and again, and for good reason. We all know scientists...sections of the relevant papers, and then read them again. Most of the time, the answers will be there. ... -
History of CRISPR Cas - A tale of survival and evolution
TypeBlog Post...Cas as a powerful and adaptive defense strategy against life-threatening viruses. Evolving to survive ...clustered repeat sequences operated as a safeguard against bacteriophages did not come to light until 2007...Cas systems provides powerful lines of defense against invading phages. Rising to the challenge - Evolution... -
Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC
TypeBlog Post...use two antibodies - one against your target of interest and a second against the first antibody. When.... (C) A third solution is to use Fab fragments against the sample species to block the binding sites on... -
Antibodies 101: Chimeric Antibodies
TypeBlog Post...and find an ideal antibody against Protein A and an ideal antibody against Protein B. The antibodies are...target and can be used with secondary antibodies against the new Fc region. In addition to increasing multiplexing... -
Summer SciComm: Science Storytelling
TypeBlog Post...said, “Science is failing over and over and over again until you don’t.” As every researcher can attest...- within that process of failing, over and over again until you don't, of trying to understand your completely...understanding why your experiment has not worked…again…for the seventh time…. I find that once I sit ... -
Scientists Map the SARS-CoV-2-Human Interaction Network
TypeBlog Post...Paris and New York. We want YOU to join the fight against coronavirus We are just at the beginning of understanding...next scientist joining in this worldwide fight against SARS-CoV-2. As we’ve all been affected in various... UCSF (the QCRG network coming to the rescue -- again!). Also, we’ve got to mention that our FedEx guy... -
Antibodies 101: Monoclonal Antibodies
TypeBlog Post... Although it’s possible to generate antibodies against many types of molecules including proteins, lipids...hybridomas come from animals that were immunized against the target protein. Animals are first injected ...special medium called HAT. This medium selects against unfused myeloma cells and antibody-producing B ... -
Luminescent Imaging with Nano-lanterns
TypeBlog Post...luminescent imaging a reality, Addgene depositor Takeharu Nagai and colleagues at Osaka University have developed...signal. Since their first publication in 2012, the Nagai laboratory has assembled a collection of multicolored...imaging. Nano-lanterns and your experiments The Nagai laboratory has a done a wonderful job characterizing... -
Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages
TypeBlog Post... preliminary injunction against the FLSA updates was granted nationwide. Again, we at Future of Research...This post discusses how a nationwide injunction against the FLSA is affecting universities' decisions to... -
Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)
TypeBlog Post...it can also be used to characterize antibodies against the antigen bound to the plate. Figure 3...absorbed by a solution - of these wells is plotted against the known protein concentration. Importantly, you...: A standard curve is generated by plotting OD against known concentrations of your target protein. The... -
Adeno Associated Virus (AAV) for Cell and Gene Therapy
TypeBlog Post...other hand, vectors derived from AAV have recently gained popularity as they are uniquely suited gene delivery...landmark study showed significant clinical efficacy against a form of blindness known as Leber’s congenital...example, prevalence of neutralizing antibodies against some AAV types, AAV1, 2, 3 and 5 are thought to...