We narrowed to 395 results for: ICL

...the blog articles come up as well as their topic category. New author pages to find articles from your... to the new Addgene blog! Same URL, same great articles to help you with your experiments, but a new look... molecular biologists alike. Now with over 600 articles, we needed a better way to organize and present...try the search bar Now, you can search for the articles you want to find without even leaving the Addgene...behind the blog post and find more of their work. Articles from guest bloggers will have the author information...information at the top and bottom of their article. We hope you’ll join the conversation! Let us know if ...you up-to-date. Be the first to know about new articles on the Addgene blog by subscribing to instant ...

...the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid... tagging of proteins for degradation using AID Article contributed by Alyssa Cecchetelli Auxin Inducible...targeting range of CRISPRCas9 by engineering SpCas9 Article contributed by Shreya Vedantam CRISPR/Cas9 is widely... channelrhodopsins for the optogenetic toolbox Article contributed by Susanna Bachle Anion-conducting ...identified MerMAIDS from their analysis of different publicly available metagenomic data including the Tara ...demonstrates the power of combining the analysis of publicly available metagenomic datasets with biophysical...PMID: 31346176. Using LADL to loop with light Article contributed by Eric Perkins Mammalian genomic DNA...

...Cas9 RNP delivery vehicle that’s based on mouse leukemia virus (MLV) virus-like particles (VLPs). VLPs lack...are engineered murine leukemia virus (MLV)-like particles loaded with Cas9-gRNA ribonucleoproteins (RNPs...their tool Nanoblades because they think of these particles as tiny DNA cutting ninjas that deliver CRISPR..., multimerize and spontaneously assemble into particles at the cell membrane. Mature Nanoblades then bud...the Gag-Cas9 fusion protein. A single Nanoblade particle can be loaded with 1-4 different gRNAs. Viral ... DNA can be generated by incubating Nanoblade particles with polybrene, a cationic polymer. Polybrene ...surface, and also helps form DNA and retroviral-particle or VLPs complexes. The team found that "all-in-one...

...notice. A 2009 research study found that 12% of articles in medicine, 27% in natural sciences, 82% in humanities... But these continue to rely on printed journal articles as the dominant tool for sharing ideas. This leads... us, such as finding time to read all of these articles. Most of us hardly have time to keep up with all... them on their own. According to a Nature News Article, since the 1980s researchers have been increasingly... outside of their disciplines. Included in the article is an interactive chart where you can see the yearly...useful tool for turning video views into online article downloads – especially if they are published in...to learn more, you can then link to the journal article, find blogs about the study, or even review Facebook...

...preps in our repository through our Hot Plasmids articles. Here's what you'll find in this post: MagIC-Cryo-EM...making it easy to identify dozens or hundreds of particles surrounding each of the highly-visible beads on...collected with MagIC-cryo-EM. Nucleosome-like particles identified by Topaz software (green circles), ... from AWI by helical LEAs. C-E) cryo-EM single-particle analysis of PRC2 with RvLEAMshort (1:6 molar ratio...glutaraldehyde crosslinking: representative micrograph (C), particle orientation distribution and Fourier-shell correlation... addition of LEAs may bias the distribution of particle orientations, this could be overcome with methods...prime editors (PEs) via engineered virus-like particles (eVLPs) offers an option to transduce cells directly...

...Physical titer (particle count, rAdV genomes/mL) can be measured by: Optical density: particle concentration...260nm corresponds to 1.1 x 1012 particles/mL. To calculate the particle number: OD260 reading x dilution...dilution factor x 1.1 x 1012 particles = number of particles per mL of sample. The purity of the sample can...vectors The adenovirus virion is a non-enveloped particle of ~100 nm, consisting of a capsid surrounding...

...the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid...Expanding the optogenetics toolbox with CRY2clust Article contributed by Brook Pyhtila  Listen to the CRY2clust...novel opsin that enables single-cell optogenetics Article contributed by Meghan Rego  Listen to the soCoChR...29184208.        Synthetic decaffeination operon Article contributed by Angela Kaczmarczyk  Listen to the... for live-cell imaging of biochemical activity Article contributed by Angela Holmes Jin Zhang’s lab is...Listen to the FLIM-FRET acceptor podcast segment Article contributed by Christina Mork The Murakoshi lab...

.... Physical titers measure the amount of viral particles in a sample and are usually based on the presence...infectious titers, measure how many of the viral particles produced can actually infect cells. These assays...whether or not it is incorporated into a viral particle. Consequently, titers based off of p24 quantification...they can include free p24 and defective viral particles. Direct measurement of lentiviral RNA is one alternative...prep as the measurement will include defective particles. Studies have found that values can be 10-1000...quantify virus as it only measures infectious viral particles. One of the si mplest methods of measuring functional...distinguish between a cell that has had one viral particle integrated into its genome and a cell that has...

...a lot of time in PubMed searching for research articles? Well then, my scientist friend, do I have a handy...few quick steps, you can use PubMed to see which articles contain plasmids deposited at Addgene from the... Plasmids” badge, you’ll know that within that article you’ll find new plasmids. Simply click on the badge...site (Side note: I always recommend you read the article to ensure you understand the features of the plasmids...log-in to your PubMed account and are browsing article abstracts you should see the “Find Plasmids” box...the Literature How to Write a Scientific Review Article Tips for Improving Your Next Manuscript Resources...

...to all articles, Anurag Acharya and colleagues report, the percentage of citations to articles in non-... on the average number of citations for recent articles. Of course, a publication in Science or Nature... citation counts for a moment, it’s clear that articles in less-prestigious journals have been gaining...Journals” found that the proportion of highly cited articles appearing in lower-prestige journals has risen...impact of research" (2013) Scientific Reports 3, Article number: 1649 doi:10.1038/srep01649    ...

...your deposit on hold from public access until the article is published. This policy gives us time to complete...as soon as we become aware the related journal article has been published we’ll get the plasmids online... is still for you to notify us as soon as your article is published. As much as we’d like to, we can’t... soon as possible, whether alongside a journal article, a preprint, or without any paper at all. Posting...update your plasmids’ citation to the final journal article version any time later. We recognize that a preprint...preprint-associated materials the way we will with journal articles. But we do encourage you to consider sharing them...

...the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid...here.  Human whole genome sgRNA iBAR library Article contributed by Susanna Bachle Figure 1: Initial...Sparse cell labeling using recombinase competition Article contributed by Aliyah Weinstein Sparse Predictive...GFP reporter for calcineurin activity in yeast Article contributed by Leah Schwiesow Calcineurin is a ...TALE-fluorescent protein fusions as a DNA staining dye Article contributed by Jennifer Tsang Figure 4: Double...

...of virus, not the individual viral particles. A single viral particle is called a virion. Finally, viral...they’re alive or not. Yet these simple, small particles have quite the outsized impact — and not just ...many After a virion infects a cell, the single particle creates many copies of itself, sometimes up to...eliminate their replicative ability. The remaining particle, which retains the protein coat (and envelope)...genetic payload to a cell but cannot create new particles. As a bonus, this increases the carrying capacity...

...the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid...MarathonRT: A highly processive reverse transcriptase Article contributed by Will Arnold  Listen to the MarathonRT...monitor retrograde transport at the cell surface Article contributed by Marcy Patrick  Listen to the nanobody...proteins occupying a specific genomic locus: Caspex  Article contributed by Susanna Bachle  Listen to the Caspex...proteins occupying a specific genomic locus: C-BERST Article contributed by Klaus Wanisch  Listen to the C-...

...the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid...microtubule-associated protein to regulate cell growth dynamics Article contributed by Klaus Wanisch  Listen to the optogenetics...for visualizing RNA production and localization Article contributed by Tyler Ford  Listen to the riboglow...tools for developing new FPs - the mCarmine story Article contributed by Caroline LaManna  Listen to the ...quantification of base-editing and Cas9 cleavage activity Article contributed by Angela Kaczmarczyk  Listen to the...

...will ensure the virus gets produced, and viral particles are packaged, when the vector is expressed. Once...expressed within the production cell line, viral particles will be produced and packaged, resulting in functional...functional virions. These viral particles can infect other cells, but since the majority of the viral ...be collected for the transduction step. Viral particles are secreted into the cellular media, making viral...like to infect with the virus. Then the viral particles do the rest! Downstream expression or integration...1: Transduction to expression process. A viral particle enters a host cell, enters the host nucleus, is...

...   Articles with and without plasmids at Addgene To find out whether scientific articles associated...bibliographic information and uses its vast network of articles to track the total number of citations the paper... of which had plasmids deposited with Addgene. Articles with Addgene plasmids get more citations So what...estimates based on the journal that published the articles.  That is, we re-estimate the citations to the...weights. Table 1: Journal Weighting Example Article Number (A) Year Published (B) Number of Citations...

...the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid...   A retroviral mouse CRISPR knockout library Article contributed by Alycia Bittner CRISPR pooled libraries...reporter for measuring ApoB bound lipoproteins Article contributed by Leah Schwiesow A leading cause of...transcription for CRISPR activation in bacteria Article contributed by Will Arnold Baojun Wang’s lab recently...Genetically encoded voltage indicators for use in worms Article contributed by Brook Pyhtila Genetically encoded...

...to as hosts) via viral particles, the functional infection unit. Each particle has a viral envelope, which...the target cell’s membrane, allowing the viral particle access into the cell. The viral envelope and its...cargo – its own genome.   Fig. 1: Viral particle infection into a host cell. Image courtesy of ... measures, like replication incompetent viral particles, are required. We will be covering these safety...localization signals which can traffic their viral particles inside the nucleus, without the need for envelope...

...the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid...efficient RNAi construct for C. elegans gene knockdown Article contributed by Alyssa Cecchetelli  Listen to the... proximity labeling with TurboID and miniTurbo Article contributed by Eric Perkins  Listen to the TurboID.... Changing the way we detect viral outbreaks Article contributed by Izzy Mueller  Listen to the viral...collection reveals novel peptide ligand motifs Article contributed by Michael Lemieux  Listen to the SH3...