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We narrowed to 185 results for: INV-E

Showing: 41 - 60 of 185 results

  1. Antibodies 101: Conjugation

    Type
    Blog Post
    ...doi.org/10.1016/j.it.2012.02.010 Dennler P, Fischer E, Schibli R (2015) Antibody Conjugates: From Heterogeneous...doi.org/10.3390/antib4030197  Hegazy M, Cohen-Barak E, Koetsier JL, et al (2020) Proximity Ligation Assay...Reisfeld RA (1988) Determination of the Number of e-Amino Groups Available for Conjugation of Effector...However, there are certainly other approaches that involve modifying the antibody sequence or that rely on...

  2. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    ...isothermal amplification by primers that target the E and the N2 gene of SARS-CoV-2. If the Cas12a-sgRNA...CRISPR-based SHERLOCK (Specific ​H​igh ​s​ensitivity ​E​nzymatic ​R​eporter un​LOCK​ing) technique Sensitivity...Granados A, Sotomayor-Gonzalez A, Zorn K, Gopez A, Hsu E, Gu W, Miller S, Pan C-Y, Guevara H, Wadford DA, Chen...doi.org/10.1101/2020.02.29.971127 Rauch, J. N., Valois, E., Solley, S. C., Braig, F., Lach, R. S., Baxter, N...University. Shravanti Suresh is a PhD candidate who investigates cellular aspects of CRISPR/Cas immune systems...

  3. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    ...Volak, A., Spirig, S. E., Muller, A., Sousa, A. A., Tsai, S. Q., Bengtsson, N. E., Lööv, C., Ingelsson...10.1101/2023.06.13.544831 Santinha, A. J., Klingler, E., Kuhn, M., Farouni, R., Lagler, S., Kalamakis, G....less work for the first two steps, as no mice are involved. Ex vivo starts with transducing cells with the...lentivirus requires a BSL-2 setup. This requires a less involved setup when working with mice and slightly less...high degree. This is particularly helpful when investigating tissue-specific diseases, as you can target ...like Cas12a (Cpf1). Even more creative options involve generating strains of mice that stably express ...

  4. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    ...thetaiotaomicron, Mycobacterium, Pseudomonas, and E. coli. However, many non-model bacterial species lack...100 to 1,000 colonies, but some bacteria, including E. faecalis and P. mirabilis, had only ~1 transfer per... PubMed Central PMCID: PMC6424567. Peters, Joseph E. "Tn7." Mobile DNA III. American Society of Microbiology...into multiple bacterial species including several involved in human disease. However, the rate of transfer...for the study of essential genes such as those involved in antibiotic resistance. Are you ready to move...

  5. Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening

    Type
    Blog Post
    ...Screening in Human Cells. Shalem O, Sanjana NE, Hartenian E, Shi X, Scott DA, Mikkelson T, Heckl D, Ebert BL, ...CRISPR-Guide RNA Library. Koike-Yusa H, Li Y, Tan E-P, Del Castillo Velasco-Herrera M, Yusa K. Nature ...melanoma model, they also went in search of genes involved in resistance to the cancer drug vemurafenib. ...lab has used their library to screen for genes involved in bacterial toxin resistance, identifying 4 previously...

  6. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...PubMed Central PMCID: PMC2600441. Foust, K.D., Nurre, E., Montgomery, C.L., Hernandez, A., Chan, C.M. and ...19098898. PubMed Central PMCID: PMC2895694. Hollis, E. R. 2nd, Kadoya, K., Hirsch, M., Samulski, R. J., ...S.F., Andrews, A.M., Lutton, E.M., Mu, D., Hudry, E., Hyman, B.T., Maguire, C.A. and Ramirez S.H. (2017... Wakeman, W., Li, Y., Feng, D., Ho, A., Nicholas, E., Hirokawa, K.E., Bohn, P., Joines, K.M., Peng, H....Central PMCID: PMC5102064. Roberts, T. F., Hisey, E., Tanaka, M., Kearney, M., Chattree, G., Yang, C. ... Ritola, K.D., Lindo, S., Michael, S., Kuleshova, E., Ojala, D., Huang, C.C., Gerfen, C.R., Schiller, ...hippocampus and the prefrontal cortex brain regions is involved in the formation of episodic memory, a special...

  7. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    ...Plant vectors are usually designed to shuttle between E. coli and Agrobacterium tumefaciens, a plant-infectious...across species, for example, in both S. cerevisiae and E. coli. Auxotrophic selection is especially common ... shuttling between. For example, if you are using E. coli simply for easy cloning and quick DNA production...vectors? The production of viral vectors typically involves shuttle vectors, also sometimes called transfer...target cells. However, while shuttle vectors are involved in the production of viral vectors, we consider...

  8. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type
    Blog Post
    ...during plasmid DNA extraction and purification from E. coli is the presence of the protein endonuclease ...encoded by the gene endA. Nowadays, the most common E. coli strains used for plasmid amplification are endA...DNA preparation in any downstream application involving enzymatic reactions, a valid alternative can be...

  9. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    ..., R., Zuber, J., Connelly, S. M., Mathew, E., & Dumont, M. E. (2014). Fluorescent approaches for understanding...Jeddi-Tehrani, M., Mahmoudi, A. R., Bayat, A. A., Shaban, E., Vafakhah, M., Darzi, M., Tarahomi, M., & Ghods, ...Bhattacharya, K., Pyrgiotakis, G., Zhang, Z., Purington, E., Gramlich, W., Grondin, Y., Rogers, R., Bousfield...Stability of fluorescence: For applications that involve ranges of pH, and significant lengths of time, ...incorporation of fluorophores, as seen in studies involving dopamine D2/D3 receptors (Allikalt et al., 2020...processes like mitochondrial dynamics. This process, involving mitochondrial homeostasis maintained through mechanisms...

  10. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    ...the CRISPR sequences were initially discovered in E. coli in 1987 (1), the concept that these clustered... K, Haft DH, Barrangou R, Brouns SJJ, Charpentier E, Horvath P, Moineu S, Mojica FJM, Wolf YI, Yakunin...various cas genes not only to store a record of invading phages but to also destroy the phage upon re-exposure... the foreign DNA, a cut is made to destroy the invader. Wikipedia, accessed 25 November 2013. Author...systems provides powerful lines of defense against invading phages. Rising to the challenge - Evolution of...research. Do you have a cool idea or application that involves CRISPR/Cas technology? Let us know.  References...

  11. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    ...Biering SB,  Sarnik SA, Wang E, Zengel JR, Sathyan V, Nguyenla X, Van Dis E, Catamura C, Yamashiro LH,  ...Puschnik AS, Bertozzi CR, Carette JE, Stanley SA, Harris E, Konermann S, Hsu PD (2021) Genome-wide, bidirectional...starting point for SARS-CoV-2 researchers to investigate how the virus interacts with human cells. Addgene...

  12. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type
    Blog Post
    ...different strains of E. coli that can be used for transformation. The preferred E. coli strains for storage... DNA has been received and stored, it is put into E. coli strains that have been treated to easily take...kanamycin resistance gene. Every step along the way involves good aseptic technique and controls to ensure ...

  13. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ...amino acids E. coli and Mammalian Cells pEVOL-pAzF synthetase for p-azido-l-phenylalanine E. coli pEVOL-pBpF...has proven most effective only in a few cell types (E. coli and Xenopus oocytes, mainly). Nevertheless, ...pEVOL-pBpF synthetase for p-benzoyl-l-phenylalanine E. coli pAcBac1.tR4-MbPyl pyrrolysyl-tRNA synthetase...commercially available as Lumio Green and Lumio Red from Invitrogen (now ThermoFisher Scientific). These reagents...structurally characterizing large ion channels involved in skeletal muscle contraction.   References 1...

  14. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    ...plasmid multimerization in the naturally occurring E. coli plasmid ColE1 (Goebel & Helinski, 1968; Bazaral... (Bedbrook & Ausubel, 1976; Fishel et al., 1981). E. coli strains such as DH5α, HB101, NEB Stable, Stbl3...multiple circular DNA forms of colicinogenic factor E-1 from Proteus mirabilis. Biochemistry. 1968 Oct;7...1566524020666200427110452. PMID: 32338220. Crozat E, Fournes F, Cornet F, Hallet B, Rousseau P. Resolution...during our quality control process. Since MiSeq involves sequence assembly from short reads (<300 bp) which...

  15. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    ...Lentiviral preps for expressing the E protein (pLVX-EF1alpha-SARS-CoV-2-E-2xStrep-IRES-Puro) and NSP2 (pLVX-EF1alpha-SARS-CoV...service in 2016, we’ve added many new tools to our inventory of ready-to-use viral vectors. Here are some of...interneurons (83895). See our Serotype Testing AAV inventory for the entire collection! Plasmid Serotype...Fishell   Chemogenetics Chemogenetics offers noninvasive methods for manipulating cell activity, particularly...

  16. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    ...Ye C, Rogan SC, Adams AC, Cusher DS, Maratos-Flier E, Roth BL, Lowell BB. Rapid, reversible activation ...PubMed Central PMCID: PMC3069789. 3. Kügler S, Kilic E, Bähr M. Human synapsin 1 gene promoter confers highly...few inventory items offered domestically, but by the end of 2016, we expanded our viral inventory to 25... lentiCas9-Blast Most of Addgene’s lentiviral inventory is based on CRISPR tools, though we do provide...pAAV-hSyn-DIO-hM4D(Gi)-mCherry Our current AAV inventory consists almost entirely of viruses expressing... Carolina, Chapel Hill. These can be used to investigate the effects of activating particular signaling...neurons drives feeding behavior in mice. J Clin Invest. 2011 Apr;121(4):1424-8. PubMed PMID: 21364278....

  17. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

    Type
    Blog Post
    ...Burke Lab (Roux et al., 2012). BioID utilizes the E. coli biotin ligase, BirA*, with a site mutation (...DNA-binding domain (233 amino acids) found in the E. coli version. It also contains a single mutation ...signal to noise ratio 30-fold compared to the standard E. coli BirA* mutant. RapID consists of this mutant ...Interactions through Spatial Proximity." Journal of Investigative Dermatology 137.12 (2017): e197-e203. PubMed...

  18. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    ...00045-5 De Rie, D., Abugessaisa, I., Alam, T., Arner, E., Arner, P., Ashoor, H., Åström, G., Babina, M., Bertin... S., Montgomery, M. K., Kostas, S. A., Driver, S. E., & Mello, C. C. (1998). Potent and specific genetic...15476286.2023.2288741 Taxman, D. J., Moore, C. B., Guthrie, E. H., & Huang, M. T. (2010). Short hairpin RNA (SHRNA...silencing. RNA interference RNAi is a useful tool to investigate the roles of specific genes or to knock down ...term for a number of different applications that involve different types of small RNA molecules, such as...

  19. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ... a supplemental add-on kit and is compatible with E. coli and/or cell-free systems. Protein expression... et al. “EcoFlex: A Multifunctional MoClo Kit for E. Coli Synthetic Biology.” ACS Synthetic Biology, vol...CIDAR MoClo: Improved MoClo Assembly Standard and New E. Coli Part Library Enable Rapid Combinatorial Design...//doi.org/10.1021/acssynbio.6b00337. Lee, Michael E., et al. “A Highly Characterized Yeast Toolkit for...model organisms and backgrounds, MoClo can be invaluable for more complex editing and screening experiments...

  20. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    ...polymerase I (PolI3M) is capable of mutagenesis in E. Coli, but this approach requires special bacterial...mutations per nucleotide per generation of wild-type E. coli, while only increasing the standard mutation...be used to map protein-protein interactions, investigate non-coding segments of the genome, or engineer...
Showing: 41 - 60 of 185 results