...chromatin looping with the photo “developed” by analyzing DNA sequencing data. While “pictures” generated... the well-characterized beta-globin locus were analyzed with CAPTURE 2.0, with two gRNAs targeting each...

... FASTA format (example) and can be analyzed using Addgene’s Analyze Sequence tool. Excellent, how can...

...plasmids!), the number of sequencing results we analyze as part of our quality control process has steadily...steadily grown. On a busy week, we may need to analyze more than 200 plasmids as part of our quality control...

...in which students learn to express, purify, and analyze MDH in semester one and then proceed to use those...1: Undergraduates in Provost’s CURE prepare to analyze their recently purified MDH to study the impact...

...template; and 3) run your PCR product on a gel to analyze product size. This blog post discusses some of ...detecting contamination is also a good idea.    Analyzing PCR product size on a gel   Now that your PCR...

...specializes in developing novel technologies to analyze molecular mechanisms of genome functions such as...the inducible translocation trap (ITT) system to analyze signal-induced nuclear translocation of proteins...

...attP sites of the donor vector. This reaction is catalyzed by the BP Clonase enzyme mix and generates the...sites of the destination vector. This reaction is catalyzed by the LR Clonase enzyme mix. As a result, an ...

...DnaA doesn’t need to hydrolyze ATP to do its job — although ATP does get hydrolyzed over time, inactivating...

...Flow cytometry is a method that allows users to analyze single cells in a population. It can be used to...many variations in how one can gate, select, and analyze data. But now that you can read a FACS plot and...

...crucial for us to keep pace with our current need to analyze nearly 300 sequences each week. Our internal software...sequence that would be ready for our QC team to analyze. Criteria for a bioinformatics workflow management...

...becomes active when illuminated with blue light, catalyzing the biotinylation of nearby proteins. B) TransitID...ticker tape from cFos::GFP-iPAK4 within an hour. Analyzing the ticker tape images could recover transcriptional...

...luciferase fragment (expressed separately) assemble and catalyze the luminescence reaction (Figure 5). The researchers...under tension. Upon binding, LgBiT and BiT Tag catalyze a luminescent reaction with the substrate furimazine...

... good expression, you should also functionally analyze the protein produced to ensure that it has folded...have full sequence data, we recommend directly analyzing gene sequences for codon optimization and suitability...

...developed a new genome-wide CRISPR knockout screen to analyze the following unanswered questions about CRISPR...when using non-targeting guides, when both are analyzed using the same false-discovery rate cutoff.  Adding...

...directed evolution (Tei et al., 2023).  PLD can hydrolyze phosphatidylcholine (PC), the predominant membrane...labeling via click chemistry.    Figure 4: SuperPLD hydrolyzes membrane PC to PA. A) OptoPLD is recruited to...

...Learn More about Restriction Cloning Get Tips on Analyzing DNA Sequencing Results Check out Plasmid Cloning...Our Protocol Pages Find Molecular Biology Tools Analyze Your Plasmids by Restriction Digest ...

...detection signal.   Analyzing the western blot Once visible, it’s time to analyze your protein of interest...

...When analyzing your cells using flow cytometry, you are typically measuring the presence or absence of...possible. Finally, we designed a simple panel to analyze transduced HEK 293T cells. Here, we looked at both...

...Immunocytochemistry (ICC) Cell Sorting - used to identify, analyze, capture, and sort single cells. Includes: Flow...commonly in immunology, allows you to identify, analyze, capture, and sort single cells. Cells, with labeled...which the protein of interest can be isolated and analyzed. Validation relies on size comparison of peptides...

...base editors (pyrimidine-to-pyrimidine), which catalyze base transitions. Cytosine base editors are created...The TadA domain has also been further evolved to catalyze cytidine deamination instead of (or in addition...researchers have developed base editors that can catalyze base transversions. These editors are based on...C-terminus of Cas9 H840A nickase. The fusion enzyme can catalyze targeted insertions, deletions, and all possible...