We narrowed to 98 results for: MLS

1. RANbodies: Reporter Nanobody Fusions

   Type

   Blog Post

   ...’s Developmental Studies Hybridoma Bank (DSHB). 1 ml of antibody-containing hybridoma supernatant is only...

2. Overcoming the Challenges of Lentiviral Production

   Type

   Blog Post

   ...only yield 150 μl of material as opposed to the 15 ml collected from an unconcentrated prep. With this...

3. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

   Type

   Blog Post

   ...https://doi.org/10.1038/srep31730 Tsuruta LR, dos ML, Moro AM (2018) Display Technologies for the Selection...

4. PCR Applications for Copy Number Variation Assays

   Type

   Blog Post

   ...Providenti MA, O'Brien JM, Ewing RJ, Paterson ES, Smith ML. The copy-number of plasmids and other genetic elements...

5. 5 Tips for Troubleshooting Viral Transductions

   Type

   Blog Post

   ... with half the volume typically used (e.g., use 5 mL medium on a 10 cm plate). By reducing the culture...

6. Plasmids 101: Secondary Nanobody Toolbox

   Type

   Blog Post

   ...’s Developmental Studies Hybridoma Bank (DSHB). 1 ml of antibody-containing hybridoma supernatant is only...

7. Viral Vectors 101: Preparing Pooled Libraries

   Type

   Blog Post

   ...sensitive to freeze/thaw cycles, we include a 1.3 mL aliquot with mostlibrary requests specifically to...

8. Getting to Know Expansion Microscopy

   Type

   Blog Post

   ...j.neuron.2010.11.021  Gambarotto D, Zwettler FU, Guennec ML, et al (2019) Imaging cellular ultrastructures using...

9. DNA Purification Without a Kit

   Type

   Blog Post

   ...alkaline lysis plasmid miniprep Starting material: 2 mL bacterial culture Product: plasmid DNA This kit-free...

10. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type

    Blog Post

    ...necessary. To save time and money, I like to miniprep 5 mL of my AAV transfer plasmid cultures and then screen...

11. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

    Type

    Blog Post

    ...cassette, and illustrates how the volume of the 1 ml outgrowth spread on each transformation plate can...

12. Antibiotic Resistance: An Old Solution but a New Problem

    Type

    Blog Post

    ...Guest Blogger, Jennifer Rivers! Jennifer Rivers ScM, MLS(ASCP)CM is a research microbiologist specializing...

13. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type

    Blog Post

    ...Appleton CM, White DE, Brown MT, Deshmukh H, Kemp ML, Bao G (2015) Trans-spliced Cas9 allows cleavage ...

14. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type

    Blog Post

    ...ultrasound contrast. In all the cell lines we tested, 1 µg/mL doxycycline was sufficient to saturate expression...

15. Getting the Most from Your Lentiviral Transduction

    Type

    Blog Post

    ...hard to detect with levels reaching 108 cells per ml before the media becomes cloudy. Mycoplasma compete...

16. Finding nucleic acids with SHERLOCK and DETECTR

    Type

    Blog Post

    ...Garcia KF, Barnes KG, Chak B, Mondini A, Nogueira ML, Isern S, Michael SF, Lorenzana I, Yozwiak NL, MacInnis...

17. CRISPR 101: Epigenetics and Editing the Epigenome

    Type

    Blog Post

    ...plasmids from this publication at Addgene. Maeder ML, Angstman JF, Richardson ME, Linder SJ, Cascio VM...

18. Important Considerations When Using AAVs

    Type

    Blog Post

    ...For long term storage, make > 25 μl aliquots in 0.5 ml tubes, snap freeze in liquid nitrogen or a dry ice...

19. Plasmids 101: Dimers and Multimers

    Type

    Blog Post

    ...: 18607781. Schleef M, Baier R, Walther W, Michel ML, Schmeer M. Long-term stability study and topology...

20. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type

    Blog Post

    ...https://doi.org/10.1534/genetics.114.170423 Schwartz ML, Jorgensen EM (2018) SapTrap vectors for introducing...