... for transformations. Con: Expensive.   Kanamycin Kanamycin, or kan, is an aminoglycoside which inhibits...Ampicillin Fair {% icon icon_set="fontawesome-5.14.0" name="Check" style="SOLID" height="16" purpose="decorative...Just bacteria {% icon icon_set="fontawesome-5.14.0" name="Check" style="SOLID" height="16" purpose="decorative...decorative" title="Check icon" %} Satellite colonies Kanamycin Excellent No Bacteria and eukaryotes (G418) {%...{% icon icon_set="fontawesome-5.14.0" name="Check" style="SOLID" height="16" purpose="decorative" title...Check icon" %} {% icon icon_set="fontawesome-5.14.0" name="Check" style="SOLID" height="16" purpose="decorative... Excellent {% icon icon_set="fontawesome-5.14.0" name="Check" style="SOLID" height="16" purpose="decorative...

... fully differentiated body. In contrast, these dynamic processes are largely absent in adult organisms... of an immune response, but the study of these dynamics was still laborious as epifluorescence microscopy... cellular motility, interactions, and response dynamics. In the past 15 years efforts have been made to...scientists have been able to decypher cellular dynamics in lymphoid organs. In lymph nodes, for example...have been successfully used to study B and T cell dynamics within the germinal center of murine lymph nodes...used in fate mapping experiments. Immune response dynamics are much better understood thanks to these technologies...our understanding of the immune system and its dynamics. References 1. Peters, Nathan C., et al. "In ...

... AAV8 AAV9 Retrograde AAV PHPeB Clear Filters ID Name Receptor and Activity Reporter/Tag Cre requirement...

...tested for activity as ZFNs in zebrafish. Target Gene Name Zinc Finger Arrays Engineering Method Full Target...

...have a name. Last month, we collected suggestions for names and we’ve recently chosen four names as finalists...friend finally has a name! We thank you for your participation in suggesting names and voting in our poll...meaningful names…. Watch the video to find out the final four! (Note: For cases where the same name was submitted...love the way the names Aavery, Addy, and Cappy play on viral vector terms. And, the name Kendall has a special...

...scripts. Naming conventions Give every experiment a number: Numbers are a short, easy way to name files ...a given day. For example, instead of naming a file “output” name the file “1_output” so that you know ...not separated by spaces. File names with more than one word should be named in the camelCaseFormat since... file system in the computer and is just another name for a folder. For instance, your Desktop directory.... This can be a simple .txt document. Record the name, date, and location of all analyses (see above)....camelCase: In camelCase, each subsequent word in a file name after the first word begins with a capital letter...for a single use, save the script as originalName_descriptionOfEdit in the same directory where it was ...

...when the protein is present in a sample--just to name a few. Traditional sources of antibodies for research...

...BioRender.com. The original prime editing enzyme is named PE1 . PE1 and most other prime editors use the Moloney...

...Description Plasmid Name Use a descriptive plasmid name and match the name as it appears in its publication... it in the format: labname_addgene_batch_upload.xls Replace ‘labname’ with the name of your PI and send...chloramphenicol, erythromicin, gentamicin, hygromycin, kanamycin, nourseothricin (clonNat), spectinomycin, streptomycin...with the following information: Addgene account username. In order for the plasmids to appear in your Addgene...Plasmids, we need to know your Addgene account username. Shipping address and phone number. Plasmid sequences...Hold for Publication or Distribute pending QC. The name of the Principal Investigator and Organization where...

... both Cre and Flp dependent. Plasmid Serotype Name 124603 AAV9 pAAV-EF1a-DIO-ChrimsonR-mRuby2-KV2.1...inventory for the entire collection! Plasmid Serotype Name 51502 AAV5 pCAG-FLEX-EGFP-WPRE 114471 AAV1, ...Biosensor AAV Calcium sensors Tracking calcium dynamics that govern such activities as neurotransmitter...AAV Encoded Calcium Sensors). Plasmid Serotype Name 104489 AAVrg pGP-AAV-syn-jGCaMP7b-WPRE 104493...offering medium apparent affinity. Plasmid Serotype Name 113050 AAV9, AAVrg pAAV-hSyn-GRAB_DA1h 113049...has been packaged into AAVrg. Plasmid Serotype Name 106174  AAVrg pAAV.hSynapsin.SF-iGluSnFR.A184S...website to access them! DREADDs Plasmid Serotype Name  44361  AAV1 pAAV-hSyn-DIO-hM3D(Gq)-mCherry   ...

...sequential IDs.   Name – we encourage the use of descriptive plasmid names and using the exact names listed in...Plasmid to this Article” (left) and entering a plasmid name,plasmid type, and plasmid description/experimental... purpose (right). On the article page, enter the name, type, and description of each plasmid associated...as explained below: Pro Tip! Add all the plasmid names and descriptions first before entering the data....the intended purpose of the plasmid. Once you've named and described all of the plasmids in your deposit... not appear for empty backbones Please enter the name of any gene contained within your insert (or targeted...auto-populated list, try entering the gene's Official Full Name or one of its "Also known as" aliases in the Gene...

...NGG) 42875 pCRISPR BsaI E. coli, S. pneumoniae Kanamycin none, need Cas9 plasmid Marraffini 42876 pCas9...

...vector name and serotype you’d like to be notified about. Chemogenetics Plasmid Serotype Name 44362...inventory for the entire collection! Plasmid Serotype Name Depositor 27056 AAVrg pAAV-Ef1a-DIO EYFP Karl ...through the mDlx enhancer element. Plasmid Serotype Name Depositor 75470 AAV1 pAAV-CAG-FLEXFRT-ChR2(H134R...and promotes neuronal firing. Plasmid Serotype Name Depositor 44361 AAV1 pAAV-hSyn-DIO-hM3D(Gq)-mCherry..., is also available in AAV1. Plasmid Serotype Name Depositor 69570 AAV1 pAAV-EF1a-N-CretrcintG Connie...pAAV-hDlx-GqDREADD-dTomato-Fishell-4 Controls Plasmid Serotype Name 114471 AAV2 pAAV-Ef1a-fDIO mCherry 83900 AAVrg...-GFP-Fishell-1 Biosensors Plasmid Serotype Name 83899 AAVrg pAAV-mDlx-GCaMP6f-Fishell-2  ...

...Constitutive (non-cre-dependent) Clear Filters ID Name Promoter Fluorophore/Tag Activity Serotype PI 37825...

... concentration lies within the dynamic range of the curve. The dynamic range is the linear span between...concentrations that the curve can accurately measure.  Dynamic range depends on a number of factors, including...instrument being used to measure the response. The dynamic range needs to be wide enough to cover all possible...; however, it should not be too broad. A broad dynamic range covers a wider span of concentrations but...measurements for the unknown sample fall outside of the dynamic range of the curve, then the assay is invalid. ...linear portion of the graph. It does restrict the dynamic range of your standard curve, so if your sample...ahead of time to see what factors may impact the dynamic range of the assay. Try to minimize these.  Review...

...publication. Next up, we have the plasmid name: This is the name given to the construct by the depositing...other plasmids with similar names in the collection, but you can use this name to find this plasmid from... I know who to acknowledge? Depositing lab: The name of the Principal Investigator (PI) in whose lab ...lab the construct was made. The depositor name links to a page listing all of all this PI’s plasmids that...optimal growth temperature are listed, as well as the name of the strain.  Most Addgene plasmids are maintained...

...you started! Dear Dr./Professor [last name], My name is [full name]. I am a [type of scientist] who recently...earned my PhD from the Department of [name of the department] at [name of the university].During my thesis...writing to apply for [title of the position] at [name of the company/organization]. I am a [adjective ...talks at conferences and taught for five years at [name of the university]” Include keywords from the job...

...system ( 68292 ), they were able to demonstrate unambiguous site-specific incorporation of phosphoserine ...

...happy to learn that many DNA-based reagents are named for soup ingredients! From pSOUP to mCherry, satisfying...know this best, as most fluorescent aptamers are named for a fruit or vegetable! What is an RNA aptamer...aptamers permit the study of intracellular RNA dynamics. Aptamers are 10-100 base nucleic acid oligos ...using Spinach2 showed its ability to track RNA dynamics in a model of Fragile-X tremor/ataxia syndrome...been used to investigate intracellular viral DNA dynamics in neuronal cells (Nilaratanakul et al., 2017)...For dessert (last but not least), the only fruit-named aptamer, Mango, binds to multiple derivatives of... Jaffrey. "A superfolding Spinach2 reveals the dynamic nature of trinucleotide repeat–containing RNA."...

...the vector name and serotype you’d like to be notified about. Controls Plasmid Serotype Name 114472...Serotype Name 119036  AAV1  pAAV-hsyn-flex-Voltron-ST Chemogenetics Plasmid Serotype Name 75033...manipulation Recombinases Plasmid Serotype Name 50363  AAV5  AAV phSyn1(S)-DreO-bGHpA 121675...pAAV-EF1a-Flpo Calcium sensors Plasmid Serotype Name 104496  AAV1  GP-AAV-CAG-FLEX-jGCaMP7f-WPRE AAV1...