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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 228 results for: PID

Showing: 41 - 60 of 228 results

  1. A Deep Dive into BioBuilder

    Type
    Blog Post
    ...students to explore how engineering fields rely on rapid prototyping to develop and test hypotheses. Students...Students then use cellular extracts as a means to rapidly prototype DNA circuits and logic gates. This BioBuilder-Caltech...

  2. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    ...fluorescent protein (RFP), named GEMCherry2, that can rapidly and efficiently evaluate Cas9 cutting sites and... GEmCherry2 reporter systems provide efficient, rapid, and real-time read-outs of Cas9/gRNA efficiency...

  3. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    ...synthetase introduced to the bacteria using plasmids. Rapid expansion occurred, with hundreds of new ncAAs and... any number of experiments with. The catch The rapid expansion of GCE led to the creation of many new...

  4. Neurodegeneration Research Collection

    Type
    Collection
    ...portfolio of biomolecular tools and emphasizes their rapid and broad dissemination to the research community...available to the scientific community at low cost, with rapid delivery. Includes antibodies, viral vectors, animal... new window) An organization with a mission to rapidly develop therapeutics that will slow the progression...

  5. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    ...chromosomes is partially or completely missing in women. Bicuspid aortic valve (an aortic valve that has two leaflets...study is to understand why women with TS develop bicuspid aortic valve at a rate that is 50-100 times higher...

  7. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    ...cell line containing an activating mutation in epidermal growth factor receptor (EGFR) with the ClonTracer...distinct evolutionary paths to become resistant to epidermal growth factor receptor inhibition." Nature medicine...

  8. AAV Production in HEK293 Cells

    Type
    Protocol
    ...565-0010 (or for larger volumes 1000 mL 0.45 µm Rapid-Flow PES Filtration Unit, Nalgene 167-0045) Pro-...-Tip The pH of this solution will drift pretty rapidly upon addition of acid or base. Add only a few drops...

  9. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    ...References 1. Luo, Jinyong, et al. "A protocol for rapid generation of recombinant adenoviruses using the... the AdEasy System. 3. Jager, Lorenz, et al. "A rapid protocol for construction and production of high-capacity...

  11. CRISPR in the Clinic

    Type
    Blog Post
    ...for future CRISPR therapies CRISPR technology is rapidly evolving, and many of the challenges with therapies...delivery technologies are suited for. For example, lipid nanoparticle delivery tends to be trafficked to ...

  12. Plasmids 101: Degron Tags

    Type
    Blog Post
    ... been removed. The degradation and reversal are rapid compared to other systems because the system modulates...Cleveland DW. Inducible, reversible system for the rapid and complete degradation of proteins in mammalian...

  13. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    ...degron (AID) tag to generate proteins which can be rapidly and reversibly degraded after the addition of auxin...Central PMCID: PMC4974478. Natsume, Toyoaki, et al. “Rapid Protein Depletion in Human Cells by Auxin-Inducible...

  14. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    ...5’-phosphorylated ssDNA guides, which might be rapidly degraded by the cellular machinery, leaving a small...system, but the genome-editing field is advancing rapidly and it wouldn’t be a surprise if someone came up...

  16. DNA Purification Without a Kit

    Type
    Blog Post
    ... References Kim, Y., & Morrison, S.L. (2009). A Rapid and Economic In-House DNA Purification Method Using...Peng, F., Huang, L., Zhao, S., & Deng, W. (2018). Rapid Regeneration and Reuse of Silica Columns from PCR...

  17. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    ...References 1. Jones, Douglas H., and Bruce H. Howard. "A rapid method for recombination and site-specific mutagenesis..., Peter, Monica Winkler, and Wilfried Bautsch. "Rapid cloning by homologous recombination in vivo." Nucleic...

  18. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ...lateral flow strips are easy to transport and work rapidly, providing reliable results in as little as an ...within one hour. DETECTR thus has the ability to rapidly detect nucleic acids with high selectivity and ...

  19. Bioinformatics at Addgene

    Type
    Blog Post
    ...various pieces were connected, as this enabled us to rapidly iterate and optimize our pipeline. After reviewing...plasmid sequences on a 96-well plate basis, but for rapid testing and refinements, we specified a select small...

  20. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...interest) and mScarlet-I3 for scenarios requiring rapid maturation and prolonged imaging with minimal background...2024). A dual-plasmid CRISPR/Cas9-based method for rapid and efficient genetic disruption in Mycobacterium...
Showing: 41 - 60 of 228 results