Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 229 results for: PID

Showing: 41 - 60 of 229 results

  3. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    ...have a thinner peptidoglycan membrane and an outer lipid membrane. Common gram negative bacteria include ...negative bacteria, the alcohol destroys the outer lipid membrane, and the thin inner peptidoglycan membrane...

  4. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    ...figure below). Subsequent exposure to red light rapidly deactivates the system. Voigt’s lab used this system...GFP reporter. Subsequent exposure to red light rapidly deactivates the system. Version 1 of this far-red...

  5. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    ...fluorescent protein (RFP), named GEMCherry2, that can rapidly and efficiently evaluate Cas9 cutting sites and... GEmCherry2 reporter systems provide efficient, rapid, and real-time read-outs of Cas9/gRNA efficiency...

  6. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    ...synthetase introduced to the bacteria using plasmids. Rapid expansion occurred, with hundreds of new ncAAs and... any number of experiments with. The catch The rapid expansion of GCE led to the creation of many new...

  7. A Deep Dive into BioBuilder

    Type
    Blog Post
    ...students to explore how engineering fields rely on rapid prototyping to develop and test hypotheses. Students...Students then use cellular extracts as a means to rapidly prototype DNA circuits and logic gates. This BioBuilder-Caltech...

  8. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    ...chromosomes is partially or completely missing in women. Bicuspid aortic valve (an aortic valve that has two leaflets...study is to understand why women with TS develop bicuspid aortic valve at a rate that is 50-100 times higher...

  10. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    ...cell line containing an activating mutation in epidermal growth factor receptor (EGFR) with the ClonTracer...distinct evolutionary paths to become resistant to epidermal growth factor receptor inhibition." Nature medicine...

  11. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...respond to acute oxidative stress through membrane lipid peroxidation, cytosolic release of CAVIN1, and downstream...Single fluorescent protein biosensor for maltose Rapid construction of metabolite biosensors using domain-insertion...Single fluorescent protein biosensor for trehalose Rapid construction of metabolite biosensors using domain-insertion...sensors A Genetically Encoded Fluorescent Sensor for Rapid and Specific In Vivo Detection of Norepinephrine...

  12. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    ...References 1. Luo, Jinyong, et al. "A protocol for rapid generation of recombinant adenoviruses using the... the AdEasy System. 3. Jager, Lorenz, et al. "A rapid protocol for construction and production of high-capacity...

  14. CRISPR in the Clinic

    Type
    Blog Post
    ...for future CRISPR therapies CRISPR technology is rapidly evolving, and many of the challenges with therapies...delivery technologies are suited for. For example, lipid nanoparticle delivery tends to be trafficked to ...

  15. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    ...degron (AID) tag to generate proteins which can be rapidly and reversibly degraded after the addition of auxin...Central PMCID: PMC4974478. Natsume, Toyoaki, et al. “Rapid Protein Depletion in Human Cells by Auxin-Inducible...

  16. Plasmids 101: Degron Tags

    Type
    Blog Post
    ... been removed. The degradation and reversal are rapid compared to other systems because the system modulates...Cleveland DW. Inducible, reversible system for the rapid and complete degradation of proteins in mammalian...

  17. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    ...5’-phosphorylated ssDNA guides, which might be rapidly degraded by the cellular machinery, leaving a small...system, but the genome-editing field is advancing rapidly and it wouldn’t be a surprise if someone came up...

  19. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    ...References 1. Jones, Douglas H., and Bruce H. Howard. "A rapid method for recombination and site-specific mutagenesis..., Peter, Monica Winkler, and Wilfried Bautsch. "Rapid cloning by homologous recombination in vivo." Nucleic...

  20. DNA Purification Without a Kit

    Type
    Blog Post
    ... References Kim, Y., & Morrison, S.L. (2009). A Rapid and Economic In-House DNA Purification Method Using...Peng, F., Huang, L., Zhao, S., & Deng, W. (2018). Rapid Regeneration and Reuse of Silica Columns from PCR...
Showing: 41 - 60 of 229 results