We narrowed to 231 results for: PID

...of each construct. This new tool kit allows the rapid generation of stable transgenic cell lines which..., et al. Optimized Sleeping Beauty transposons rapidly generate stable transgenic cell lines. Biotechnol...

..., Paulk NK (2019) Fast-Seq: A Simple Method for Rapid and Inexpensive Validation of Packaged Single-Stranded...Moullier P, Ayuso E, Vandenberghe LH (2017) AAV-ID: A Rapid and Robust Assay for Batch-to-Batch Consistency ...

...homologes are functionally conserved, they develop rapidly, they are small, and their transparency allows ... hypothesizes that this may be due to different lipid compositions of C. elegans membranes, which might...

...have a thinner peptidoglycan membrane and an outer lipid membrane. Common gram negative bacteria include ...negative bacteria, the alcohol destroys the outer lipid membrane, and the thin inner peptidoglycan membrane...

...to tackle a controversial and ethically fraught rapidly emerging technology. We could do this for CRISPR...Harley and Lexi, and a clutter ofoutdoors-only spiders, all of whom are named Charlotte, all of whom are...

...figure below). Subsequent exposure to red light rapidly deactivates the system. Voigt’s lab used this system...GFP reporter. Subsequent exposure to red light rapidly deactivates the system. Version 1 of this far-red...

...single mammalian expression vector A tool kit for rapid cloning and expression of recombinant antibodies...2006 Dec 7;6:46. John McCafferty CRISPR system to rapidly engineer the constant immunoglobulin domains to...

...tetrazine ncAAs Bacterial TAG Ryan Mehl 217361 pIDTSmart-MbPylRS PylRS M. barkeri azidolysine (AzK) Mammalian...AzK) Mammalian TAG Abhishek Chatterjee 217365 pIDTSmart-CMV-PLRS1 LeuRS E. coli Mammalian Abhishek Chatterjee...L-lactyl-lysine (Klac) Bacterial TAG Nanxi Wang 240124 pIDTsmart-MbPylRS-4xPyOtR-ITR-GFP PylRS M. barkeri Azidolysine...

...students to explore how engineering fields rely on rapid prototyping to develop and test hypotheses. Students...Students then use cellular extracts as a means to rapidly prototype DNA circuits and logic gates. This BioBuilder-Caltech...

...fluorescent protein (RFP), named GEMCherry2, that can rapidly and efficiently evaluate Cas9 cutting sites and... GEmCherry2 reporter systems provide efficient, rapid, and real-time read-outs of Cas9/gRNA efficiency...

...synthetase introduced to the bacteria using plasmids. Rapid expansion occurred, with hundreds of new ncAAs and... any number of experiments with. The catch The rapid expansion of GCE led to the creation of many new...

...chromosomes is partially or completely missing in women. Bicuspid aortic valve (an aortic valve that has two leaflets...study is to understand why women with TS develop bicuspid aortic valve at a rate that is 50-100 times higher...

...what if we had a simple tool that allowed us to rapidly decode the complexity? The CombiGEM-CRISPR technology...for genome editing in large-scale has led to the rapid generation of genome-wide gRNA libraries that knock...

...cell line containing an activating mutation in epidermal growth factor receptor (EGFR) with the ClonTracer...distinct evolutionary paths to become resistant to epidermal growth factor receptor inhibition." Nature medicine...

...orange-red fluorogen A split fluorescent reporter with rapid and reversible complementation. Tebo et al. Nat ... for chromophore maturation, splitFAST displays rapid and reversible complementation. TagBiFC (split HaloTag...

...for future CRISPR therapies CRISPR technology is rapidly evolving, and many of the challenges with therapies...delivery technologies are suited for. For example, lipid nanoparticle delivery tends to be trafficked to ...

...References 1. Luo, Jinyong, et al. "A protocol for rapid generation of recombinant adenoviruses using the... the AdEasy System. 3. Jager, Lorenz, et al. "A rapid protocol for construction and production of high-capacity...

...degron (AID) tag to generate proteins which can be rapidly and reversibly degraded after the addition of auxin...Central PMCID: PMC4974478. Natsume, Toyoaki, et al. “Rapid Protein Depletion in Human Cells by Auxin-Inducible...

... been removed. The degradation and reversal are rapid compared to other systems because the system modulates...Cleveland DW. Inducible, reversible system for the rapid and complete degradation of proteins in mammalian...

...5’-phosphorylated ssDNA guides, which might be rapidly degraded by the cellular machinery, leaving a small...system, but the genome-editing field is advancing rapidly and it wouldn’t be a surprise if someone came up...