We narrowed to 255 results for: PORA

...expression, sometimes incorporating chemically inducible approaches to provide temporal control, this new ...can deliver these components together using electroporation. In these bulk cell experiments, Clift et al...no stress or damage upon TRIM-Away, and the electroporated cells re-adhered to the culture plate within...

...gene incorporated into their genome. While transgenic mice have the new gene construct incorporated randomly...embryonic stem cells. Non-homologous recombination incorporates this DNA randomly into the mouse genome. In ...homologous recombination. A targeting vector is electroporated into embryonic stem cells and this will insert...

...FPs) were derived from natural photoreceptors incorporating biliverdin IXα (BV) autocatalytically. Fortunately... a cell and depends on the efficiency of BV incorporation by an apoprotein and protein expression level...Oliinyk et al., 2019). They were engineered to incorporate BV. Their advantages are their small size (17...Michnick, S.W. An infrared reporter to detect spatiotemporal dynamics of protein-protein interactions. Nat...

... homology arms and a selection marker, then electroporate it into mouse embryonic stem cells. After selecting...compatible with standard microinjection and electroporation methods using in mouse transgenics. Designing...between the ampulla and infundibulum. In vivo electroporation is performed immediately after injection. Image...tweezer-type electrodes are placed to mediate electroporation. Ohtsuka et al. compared traditional microinjection...

...enrich and select for cells that had successfully incorporated the desired mutation, and then derive mice from...handling embryos ex vivo, the components can be electroporated into the oviduct of pregnant females, a technique...gRNA, Cas9, and donor oligonucleotide can be electroporated into the mouse zygote (Qin et al., 2015).  ...-Mediated Genome Editing in Mice by Zygote Electroporation of Nuclease. Genetics 200:423–430 . https:/...

... fluorescence range to better study the spatio-temporal dynamics of apoptosis in living animals. To do...zebrafish, the Shu Lab used FlipGFP to track the spatiotemporal dynamics of apoptosis during embryo development...allow scientists to observe both the spatial and temporal dynamics of apoptosis both in vitro and in living...

...plasmid DNA transiently (similar to bacteria) or incorporate the genetic material directly into the genome...population of cells that have fully and successfully incorporated foreign genetic material into their genomes....should be a selective pressure to force cells to incorporate the plasmid DNA into the genome. For the purposes...

...producing long unspecific RNA fragments that are incorporated into the dsRNA necessary for RNAi. The Vellai...adjacent to the T7 promoters to prevent the incorporation of non-specific nucleotides into the dsRNA leading...enzyme and a small-molecule substrate to map the spatiotemporal distribution and interaction networks of specific...

...2017). However, Acr inhibition is spatially and temporally ubiquitous, making them a rather blunt instrument...versatility opens up the potential for spatial and temporal control in many genetic targeting and editing ...response to light, offers a way to spatially and temporally control cellular processes in vivo. Many photoactive...

...Universities need to incorporate these methods into their curricula. Corporations need to integrate these...Governments, universities, and, increasingly, corporations in the US and Western Europe have taken three...

...promoters. Moving to an in vivo system, Tang et al. electroporated T-DDOGs, GFP, and a reporter construct into...TdTomato, whose expression was absent without electroporated GFP. They then tested T-DDOGs with two mouse...robust and specific. When tested in retinal electroporation studies, DsRed reporter expression was induced...

...networks’ of 157 super enhancers.  3. Measuring temporal changes in chromatin interactions A third application...application for CAPTURE 2.0 is detection of temporal changes in chromatin conformation, like those that ...Multiplexed capture of spatial configuration and temporal dynamics of locus-specific 3D chromatin by biotinylated...

...typically require an exact DNA sequence to be incorporated at a precise genomic location, with little to...template for repair, which will result in the incorporation of your sequence, a.k.a a knock-in! So how does...molecule is used as the template, you need to incorporate homologous sequence to the right and/or left ...

...transcyclooctene–tetrazine)– Nonnatural amino acids can be incorporated into a protein structure and then used with ...attach a fluorophore at the specific site of incorporation (Figure 1d). Fluorogen activating proteins...inside living cells. We recently discovered that incorporation of four-membered azetidine rings could substantially...

... editing efficiencies in vitro and in vivo. Incorporating reverse transcriptase (RT) variants, PE6a and... more. For added precision, the authors also incorporated LOV-Turbo into TransitID experiments.  Find ...in light-regulated LOV-Turbo enables precise spatiotemporal control of proximity labeling in living cells...

...research is the inability to replicate the spatio-temporal precision with which signaling events occur in...low-intensity blue light. This approach relies on the incorporation of light-oxygen-voltage (LOV) sensing domains...tissues of the same animal. Flipbow additionally incorporates SUMO tags in the FP sequence for separation ...adeno-associated viral (AAV) system enables greater spatio-temporal control over expression and increases the number...

...targets in translational research Researchers can incorporate HiBiT with a wide range of workflows, from classic...paper for more detail on whether the authors incorporated HiBiT by CRISPR tagging at the endogenous locus...2411. https://doi.org/10.1111/bph.15426 Promega Corporation (2025). Illuminating Transmembrane Proteins Using...

...pronuclei of fertilized eggs, which leads to random incorporation of that sequence into the zygote’s genome, a...unpredictable. Transposons, however, efficiently incorporate into the zygote’s genome after injection into...Sandoval-Villegas N, Nurieva W, Amberger M, Ivics Z (2021) Contemporary Transposon Tools: A Review and Guide through...

...Desktop Genetics .For ssODN design, remember to incorporate PAM-blocking mutations (e.g. convert the NGG ...into cells. Optimization of delivery methods (electroporation, lipofection etc) is the most crucial aspect...includes selection of transfection buffer and electroporation pulse program to achieve high transfection ...

... DNA to favor the edit Once the prime editor incorporates the edit into one strand, there’s a mismatch...MutSα–MutLα mismatch repair (MMR) complex, to temporarily inhibit this process and promote copying of the...it from hybridizing with the unedited strand. Temporarily inhibiting this complex may allow time for 5′...