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We narrowed to 257 results for: PORA

Showing: 41 - 60 of 257 results

  1. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

    Type
    Blog Post
    ...of Brainbow, Brainbow 3.0 (Cai et al., 2013), incorporates the highly photostable fluorophores mOrange2...default, they are not fluorescent. Instead, they incorporate a truncated, non-fluorescent form of YFP as the...overcame the low expression of XFP of Brainbow by incorporating a Tet-Off system to boost XFP expression. The...Tetbow can be introduced in vivo either by electroporation of plasmids, or using AAV vectors. The Imai... lab also developed a version of Tetbow that incorporates chemical tags instead of XFP to optimize it ...

  2. Which Fluorescence Microscopy Techniques is Best for Me?

    Type
    Blog Post
    ...the first fluorescence microscopy technique to incorporate optical sectioning. Optical sectioning refers...their fast movements. Depending on the sample, a temporal resolution on the millisecond scale may be needed...resolution due to the lack of a requirement for high temporal imaging speeds. Standard fluorescence widefield...sectioning, imaging over large distances and high temporal resolution. Traditionally, this type of sample...technique for many of these samples due to its fast temporal resolution and decreased phototoxicity. Lightsheet...

  3. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

    Type
    Blog Post
    ...you like the gene to be incorporated into the genome? Some vectors incorporate into the genome randomly... of interest in a plasmid and get the vector incorporated into the germline. To begin this process, you...pick out flies where your transgene has been incorporated into the germline. Outsourcing the plasmid injections...

  4. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    ... optogenetics is renowned for enabling precise temporal and spatial control. Optogenetics uses genetically... on and off gene expression while imparting spatiotemporal control, reversibility, and repeatability. ...codon, TAG. (To learn more about site-specific incorporation of amino acids using pyrrolysl tRNA synthetase...) A Singular System with Precise Dosing and Spatiotemporal Control of CRISPR‐Cas9. Angew Chem Int Ed 58...Rauen RR, Kahn SI, Tsang M, Deiters A (2020) Spatiotemporal Control of CRISPR/Cas9 Function in Cells and...

  6. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    ...gene incorporated into their genome. While transgenic mice have the new gene construct incorporated randomly...embryonic stem cells. Non-homologous recombination incorporates this DNA randomly into the mouse genome. In ...homologous recombination. A targeting vector is electroporated into embryonic stem cells and this will insert...

  7. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ...FPs) were derived from natural photoreceptors incorporating biliverdin IXα (BV) autocatalytically. Fortunately... a cell and depends on the efficiency of BV incorporation by an apoprotein and protein expression level...Oliinyk et al., 2019). They were engineered to incorporate BV. Their advantages are their small size (17...Michnick, S.W. An infrared reporter to detect spatiotemporal dynamics of protein-protein interactions. Nat...

  8. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    ... homology arms and a selection marker, then electroporate it into mouse embryonic stem cells. After selecting...compatible with standard microinjection and electroporation methods using in mouse transgenics. Designing...between the ampulla and infundibulum. In vivo electroporation is performed immediately after injection. Image...tweezer-type electrodes are placed to mediate electroporation. Ohtsuka et al. compared traditional microinjection...

  9. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ...enrich and select for cells that had successfully incorporated the desired mutation, and then derive mice from...handling embryos ex vivo, the components can be electroporated into the oviduct of pregnant females, a technique...gRNA, Cas9, and donor oligonucleotide can be electroporated into the mouse zygote (Qin et al., 2015).  ...-Mediated Genome Editing in Mice by Zygote Electroporation of Nuclease. Genetics 200:423–430 . https:/...

  10. FlipGFP, a novel fluorescence protease reporter to study apoptosis

    Type
    Blog Post
    ... fluorescence range to better study the spatio-temporal dynamics of apoptosis in living animals. To do...zebrafish, the Shu Lab used FlipGFP to track the spatiotemporal dynamics of apoptosis during embryo development...allow scientists to observe both the spatial and temporal dynamics of apoptosis both in vitro and in living...

  11. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    ...plasmid DNA transiently (similar to bacteria) or incorporate the genetic material directly into the genome...population of cells that have fully and successfully incorporated foreign genetic material into their genomes....should be a selective pressure to force cells to incorporate the plasmid DNA into the genome. For the purposes...

  14. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    ...Universities need to incorporate these methods into their curricula. Corporations need to integrate these...Governments, universities, and, increasingly, corporations in the US and Western Europe have taken three...

  15. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

    Type
    Blog Post
    ...promoters. Moving to an in vivo system, Tang et al. electroporated T-DDOGs, GFP, and a reporter construct into...TdTomato, whose expression was absent without electroporated GFP. They then tested T-DDOGs with two mouse...robust and specific. When tested in retinal electroporation studies, DsRed reporter expression was induced...

  16. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

    Type
    Blog Post
    ...networks’ of 157 super enhancers.  3. Measuring temporal changes in chromatin interactions A third application...application for CAPTURE 2.0 is detection of temporal changes in chromatin conformation, like those that ...Multiplexed capture of spatial configuration and temporal dynamics of locus-specific 3D chromatin by biotinylated...

  17. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    ...typically require an exact DNA sequence to be incorporated at a precise genomic location, with little to...template for repair, which will result in the incorporation of your sequence, a.k.a a knock-in! So how does...molecule is used as the template, you need to incorporate homologous sequence to the right and/or left ...

  18. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    ...transcyclooctene–tetrazine)– Nonnatural amino acids can be incorporated into a protein structure and then used with ...attach a fluorophore at the specific site of incorporation (Figure 1d). Fluorogen activating proteins...inside living cells. We recently discovered that incorporation of four-membered azetidine rings could substantially...

  19. Hot Plasmids - November 2023

    Type
    Blog Post
    ... editing efficiencies in vitro and in vivo. Incorporating reverse transcriptase (RT) variants, PE6a and... more. For added precision, the authors also incorporated LOV-Turbo into TransitID experiments.  Find ...in light-regulated LOV-Turbo enables precise spatiotemporal control of proximity labeling in living cells...

  20. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...research is the inability to replicate the spatio-temporal precision with which signaling events occur in...low-intensity blue light. This approach relies on the incorporation of light-oxygen-voltage (LOV) sensing domains...tissues of the same animal. Flipbow additionally incorporates SUMO tags in the FP sequence for separation ...adeno-associated viral (AAV) system enables greater spatio-temporal control over expression and increases the number...
Showing: 41 - 60 of 257 results