...some of the new plasmids, antibodies, and viral preps in our repository through our Hot Plasmids articles...facilitate SARS-CoV-2 research New viral vector preps of Voltron2 voltage sensors AAV vectors for targeting...voltage sensors By Brian O’Neill New viral vector preps expressing the Voltron2 voltage indicator, created...Disease, including plasmids, antibodies, viral vector preps, CRISPR tools, and more.  ...

...service provides ready-to-use AAV and lentiviral preps! Download the green AAV background.       Some ....         We also have ready-to-use lentiviral preps for COVID-19 research, pooled CRISPR lentiirus libraries...

...have newly-available AAV preparations added this year. We’ve added new viral preps of the dopamine sensor...2022.   Find iGluSnFR3 plasmids here! Find viral prep here: pAAV.hSyn.FLEX.iGluSnFR3.v857.PDGFR.codonopt...license.   Find JEDI-2P plasmids here! Find viral preps here: pAAV-EF1a-DIO-JEDI-2P-WPRE and pAAV-EF1a-DIO-JEDI...Biosensors going viral! In addition to the viral preps noted above for iGluSnFR3 and JEDI-2P, several biosensors...activity of specific RTKs are limited. In a new preprint, Jared Toettcher and colleagues describe pYtags...

...We began distributing ready-to-use virus preps through our viral service in late 2016 and requests are...pAAV-hSyn-DIO-hM4D(Gi)-mCherry was the top requested AAV prep for the 2nd year running, and you can learn more...

...noticed that there are many options to elute your DNA prep. You might see protocols that recommend eluting ...these conditions.  The next time you do a plasmid prep, plan in detail before you drop that elution buffer... buffer for preserving the stability of your preparation for a long time. Tris buffer controls the pH,... if the priority is to directly use your DNA preparation in any downstream application involving enzymatic...buffer is that it allows better control of the pH preparation stabilizing the plasmid, and still allowing most...at -20°C or even at -80°C, in this case your preparation can be eluted in water or in your buffer of preference...

...CRISPRko sgRNA library as pooled library or lentiviral prep! Dolcetto: human CRISPRi sgRNA library While CRISPRko...Dolcetto library! Now also available as a lentiviral prep. Calabrese: human CRISPRa sgRNA library In addition...Find the Calabrese pooled library and lentiviral prep here! Applications for the Root and Doench CRISPR...

...to provide ready-to-use viral preps. Here are some of the new viral preps from recent months: New GPCR...highlight a subset of the new plasmids and viral preps in the repository through our hot plasmids articles...production in E.coli as simple as following a DNA miniprep protocol!  First, the authors created the pMAK...

...the name suggests, you may also concentrate your prep to the desired concentration using these columns... this assay will also measure the purity of your prep. In this method, antibodies are separated by size...Contaminating bands lower the purity of your antibody prep. If you have multiple contaminating bands or a few...

...platform for scientists using Addgene AAV viral preps to easily find and share useful technical data. ...guide Browse all  AAV vectors Find ready-to-use AAV preps ...15 minutes or less if you have all of your data prepared. All of these questions relate to factors that...

...reagents including plasmids, ready-to-use viral preps, pooled libraries, kits, and cloning grade DNA! ...

...channel. And with no more ado, the questions!  AAV Prep and Technique Q1: Is there any way to deliver a ...you think it is possible to make an efficient AAV prep for a 4.2 kB insert with the CMV promoter using ...

...subset of the new plasmids, antibodies, and viral preps in the repository through our hot plasmids articles...pitch a Hot Plasmids section (antibodies and viral preps welcome!) here. Here's what you'll find in this...observed high fluorescence resilience to these preparations. After crystalizing the hfYFP structure, the...

...some unexpected results. Order Ready to Use Viral Preps from Addgene Measuring the pH of our samples: What... CRISPR User) Tips for Titering Your Lentivirus Preps Additional Resources on Addgene.org Check out ...

...though we do provide a few non-CRISPR related viral preps. Cas9 is the headliner of most CRISPR applications...lentivirus. Secondly, you can use a LentiCas9-Blast viral prep to create a single stable Cas9-expressing cell line...

...started. In-Stock Preps We offer commonly used AAV and lentiviral vectors as in-stock preps. These high-titer...protocols for how to use your viral prep and how to make your own preps. Recipient Instructions What to do...Service Addgene produces high-quality viral vector preps from select plasmids in our collection. We know ...high-titer preps are distributed as 100 µL solutions of purified viral particles that are suitable for in vivo...high-quality and ready-to-ship AAV or lentivirus prep for your next experiment today! Browse AAV | Browse...Request Can't find what you need in our in-stock preps? Our Packaged on Request service offers you even...eligible AAV plasmids and we’ll make the viral vector prep you need. Packaged on Request Viral Vector Packaging...

...ccdB SurvivalTM, one can efficiently propagate and prep plasmids containing the ccdB gene, which can then... strains could possibly be used to propagate and prep your ccdB-containing empty backbones but should ...

...ready-to-use viral vector preps. Today, that number has grown to over 114,600 viral preps. And in 2025, we’ll...

...service with all-in-one pricing for viral vector preps, which includes MTA facilitation, DNA amplification...protection levels. We offer 959 in-stock viral vector preps, delivering high quality and ready availability ... many researchers, scientists sometimes require preps beyond our current in-stock capabilities. To meet...start experiments as soon as they receive their AAV prep from Addgene — eliminating the steps to produce,...All-in-One Pricing The price for our Packaged on Request preps is all-inclusive of several important steps: MTA... varies by requesting organization Viral vector preps remain subject to the terms and conditions of the...priority. During this time, we amplify DNA, produce the prep, and perform comprehensive quality control processes...

...considerably lower volume than with the unconcentrated prep. For example, at Addgene we typically concentrate...opposed to the 15 ml collected from an unconcentrated prep. With this in mind, if the experiment requires ...concentrate 100-fold meaning that a 10 cm2 dish preparation of virus will only yield 150 μl of material as opposed...An optimized method for high-titer lentivirus preparations without ultracentrifugation. Sci Rep. 2015; ...

...should be prepared with different questions than those on the prep calls, but often the prep calls provide...purpose. If possible, make sure the panelists are prepared to stay around to meet and greet audience members...