We narrowed to 443 results for: RELA
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TypeCollection...in more news about optogenetics research? Find related posts on Addgene's blog. Microbial Opsin Plasmids...
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Plasmid Modification by Annealed Oligo Cloning (with Protocols)
TypeProtocol... Let's assume that your favorite vector has a relatively limited MCS (BamHI - EcoRI - SalI) and you want... -
Water Bath Protocol
TypeProtocol... your experiment. Water baths tend to heat up relatively slowly. Place the water bath cover on the top... -
Chemogenetics Plasmids
TypeCollection...to interrogate brain function and examine the relationship between neuronal signaling and behavior. Depending... -
Centrifugation
TypeProtocol...protocol. Pro-Tip Spin speed is often given as Relative Centrifugal Force (RCF) or Revolutions Per Minute... -
SARS-CoV-2 Pseudotyped Virus
TypeCollection...luciferase. Used in lentivirus-based SARS-CoV-2 related pseudovirus assay. David Nemazee 170575 pCMV-FLuc... -
Zinc Finger Consortium: Zinc Finger Arrays
TypeCollection...OPEN OPEN tCCCATCCTAtcgttGCATGAGCTc mdka (midkine-related growth factor) OZ527 and OZ528 OPEN OPEN cACCCTCCTCgtggtGCTGGTGGCc... -
Protocol - Bacterial Transformation
TypeProtocol...also make their own competent cells. This is a relatively simple procedure and is useful for performing... -
Adeno-associated virus (AAV) Plasmids
TypeCollection...contained at lower biosafety levels and elicit relatively low immunological effects in vivo . While AAVs... -
TALEN Guide
TypeCollection...Intuitively, one would expect that assembling relatively small, repeat-laden DNA regions into a single... -
Pipetting Protocol
TypeProtocol...be in the liquid. Pro-Tip If pipetting from a relatively large container holding a small volume of liquid... -
Virus Protocol - Generating Stable Cell Lines
TypeProtocol...be 10 µg/mL. Pro-Tip Transducing too many cells relative to the number of virus particles reduces the transduction... -
Coomassie Purity Stain of Recombinant Antibodies
TypeProtocol...) or a similar photo software to determine the relative intensity of the protein bands to the overall ... -
Cre-Lox and Other Site-Specific Recombinases
TypeCollection...alleles in mouse embryonic stem cells. Return to top Related Addgene Plasmid Collections AAV Enhancer Collection... -
Isolating a Monoclonal Cell Population by Limiting Dilution
TypeProtocol...hemocytometer or other cell counter. Pro-Tip Prepare a relatively dilute cell solution (<10 6 cells/mL) before ... -
Plasmids 101: Origin of Replication
TypeBlog Post...pMB1 (derivative) A Relaxed pBR322 ~15-20 pMB1 A Relaxed pET ~15-20 pBR322 A Relaxed pGEX ~15-20 pBR322... F1** A Relaxed pGEM ~300-500 pUC and F1** A Relaxed pCDF ~20-40 CloDF13 (CDF) D Relaxed This...pBR322 A Relaxed pColE1 ~15-20 ColE1 A Relaxed pR6K ~15-20 R6K* C Stringent pACYC ~10 p15A B Relaxed...Generally, control of replication is referred to as "relaxed" or "stringent" depending on whether the ori is... -
Transferable Skills Guide: External Collaborations
TypeBlog Post...conference calls and make sure to maintain these relationships by keeping in touch by email and social media...media (see the section on “Maintaining Relationships” below). Expand: Collaboration opportunities outside... fun. I just had to remember to approach the relationship with the perspective of my potential collaborators...generally asking for some advice and guidance. The relationships you establish in this way can, of course, lead...favors, but you should not go into a professional relationship expecting these things. In this context, I often...about and get started. You never know where the relationships you develop will lead! Read our blog post about... collaborations! Stay in touch: Maintaining relationships Great progress! You are collaborating with your... -
Antibody Validation for Flow Cytometry
TypeBlog Post...relying on correlations between actual antibody labeling and expected labeling. Correlation cannot prove...practice to combine correlation with other approaches when possible. Finally, correlation of -omics data ...and the abundance of the epitope in the sample, relative to cross-reactive antigens – that is, an antibody... antibody selectivity even more challenging. Correlation with RNA or Proteomic data from multiple cell...identification of the cell types of interest. By correlating your data sets, you can increase confidence that... of an orthogonal approach to understand the relationship between antibody staining intensity by flow ...frequently induce/suppress the expression of many related proteins, not just your protein of interest, which... -
Deisseroth INTRSECT Collection
TypeCollection... NOT Cre No Dual recombinase-dependent: Rabies-related Addgene ID Plasmid Logic Sites and Mutations In-Stock... -
Networking Follow-up: I Met a Person. Now What?
TypeBlog Post... build the relationship in a non-awkward way? 7 ways to strengthen your new relationships Here are some...Relationships need nurturing. Perhaps you’ve met someone interesting at an event. Or, perhaps you have...contacts you have; it’s about building genuine relationships with people you care about knowing. How do you...some ways to go about strengthening a new relationship with authenticity and not discomfort: Invite the...meeting (in person or virtually) will make the relationship stronger. If appropriate, invite them to speak...same people is often the easiest way to build relationships and strengthen them over time. Things to ...important” or senior people. Focus on building relationships with peers and people around you naturally. ...