...protein resources here . pCyPet-His 14030 FRET Patrick Daugherty FRET: Vector for arabinose inducible expression...found in pYPet-His . pYPet-His 14031 FRET Patrick Daugherty FRET: Vector for arabinose inducible expression... coli codon optimized FokI endonuclease domain. Return to Top Visualization and Tagging If you’d like ...fluorescent protein variant (CyPet) optimized for FRET measurements with a yellow fluorescent protein variant...fluorescent protein variant (YPet) optimized for FRET measurements with a cyan fluorescent protein variant...fused to the MS2 binding sites within this plasmid. Return to Top Protein Purification Sometimes looking at...multiple constructs can be made and tested at once. Return to Top Controlled Expression It isn’t always beneficial...

...transcriptional reporters. However, they are not secreted, so cells must be lysed or the substrate must ...to measure luminescence. Gaussia luciferase is secreted and more stable, making time course experiments...size. NanoLuc® Luciferase is small, bright, and a secreted version of the protein is available – these attributes...minescence‐based T wo‐ Hy brid) : Combines in vivo BRET with ex vivo luminescence-based co-immunoprecipitation...plants. Bioluminescence Resonance Energy Transfer (BRET) CalFlux VNT : An intracellular calcium sensor created...Antares and Enhanced Nano-lanterns) to allow for BRET-based in vivo imaging of multiple cellular processes...investigating regions controlling transcription Pete Stecha Return to Top Expression Constructs Luciferase driven ...

...PGKpuro2ABFP - For retroviral delivery of one sgRNA pMKO.1 GFP - Retroviral shRNA expression Retroviral backbones...Zebrafish Plasmids and Resources collection page Return to top Epitope Tag or Fusion Protein Tags and fusion...bacterial expression (Gateway) pYD1 - Expression, secretion, and display of proteins on the extracellular ...as TEV cleavable N-terminal His and NusA fusions Return to top Function Besides functions such as protein...expression, see our dedicated Lentiviral Plasmids page Retroviral Easy and safe to use, integrate into host genome...MSCV-IRES-GFP or pMSCV-IRES-mCherry FP - Mammalian retroviral gene expression with GFP or mCherry selectable...mammalian cells with the Tet-On or Tet-Off system Return to top Selectable Markers Regardless of your delivery...

...nickase) and single guide RNA 48137 : PX165; SpCas9 Return to top GeCKO Screening Library GeCKO are pooled...for more information. Full references are below. Return to top Cas9 SAM transcriptional activation plasmids...sequences Zhang Lab SAM Cloning Protocol 321.5 KB Return to top AAV - In vivo Genome Editing The Zhang Lab...fusion facilitates FACS sorting of cells and nuclei. Return to top AAV - Cas9 mouse CRISPR-Cas9 is a versatile...designed based on the target site sequence (20bp). Return to top References SpCas9 plasmids Multiplex Genome...CRISPR/Cas Systems. Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini ...10.1038/nprot.2013.143. Epub 2013 Oct 24. PubMed . Return to SpCas9 plasmids GeCKO Library Genome-scale CRISPR-Cas9...

...MMLV-derived Retrovirus Human Yamanaka factors for generating human iPS cells; retroviral expression of...MMLV-derived Retrovirus Mouse Original set of Yamanaka factors for generating mouse iPS cells; retroviral expression..., fully-differentiated adult somatic cells are returned to a pluripotent state using a cocktail of factors...11(3):373-86. Jaenisch Fibroblasts Hepatocytes Retroviral Mouse Direct conversion of mouse fibroblasts ...nature10323. Crabtree Fibroblasts Motor Neurons Retroviral Mouse/Human Conversion of mouse and human fibroblasts...2527-32. Wernig Fibroblasts Neural Stem Cells Retroviral Mouse Direct reprogramming of fibroblasts into...Cell Stem Cell. 2019 Jun 6. Buganim Glial Cells Retinal Ganglion Cells AAV/CRISPR Mouse Glia-to-Neuron ...

...expression. γ-Retrovirus Plasmids | Guide γ-retroviruses, commonly referred to as retroviruses, are the most...this infectivity is limited to dividing cells. Retroviruses integrate permanently into the host genome, ... be produced. Each of these plasmids, however, retains the properties of any typical plasmid and thus ...DNA >8 Kb 105 Dividing/Non-dividing No High γ-Retrovirus Stable RNA <8 Kb 80-130 Dividing Yes Moderate...genome integration. Community Resources Nolan Lab Retroviral Systems Trono Lab Lentivector Toolbox Guide to...

...Tth DNA Ligase 165574 pOpen-HSlig3 DNA Ligase III Return to top DNA Polymerases Addgene ID Plasmid Gene/...Polymerase 165573 pOpen-phi29pol phi29 DNA Polymerase Return to top Restriction Endonucleases Addgene ID Plasmid...165567 pOpen-XbaIR XbaI 165568 pOpen-NcoIR NcoI Return to top Reverse Transcriptases Addgene ID Plasmid...Thermus thermophilus (Tth) Reverse Transcriptase Return to top RNA Polymerases Addgene ID Plasmid Gene/...Polymerase 165576 pOpen-T3gene1 T3 RNA Polymerase Return to top Other Enzymes Addgene ID Plasmid Gene/insert...165555 pOpen-ALPI CIP (calf intestinal phosphatase) Return to top Additional Resources Bacterial Protein Expression...

...developing mouse retina. To aide in their studies of the regulatory networks involved in retinal development...plasmids to study the regulatory networks involved in retinal development. Depositor...Initiation of Otx2 expression in the developing mouse retina requires a unique enhancer and either Ascl1 or ...Simultaneous deletion of Prdm1 and Vsx2 enhancers in the retina alters photoreceptor and bipolar cell fate specification...

...8, rg* Deisseroth *AAVrg = retrograde serotype, produced with the AAV retro helper plasmid from the Karpova...5, 8, 9, rg* Engel , Nectow 69570 pAAV-EF1a-N-CretrcintG EF1a none 1 Cepko 69571 pAAV-EF1a-C-CreintG EF1a...Karpova and Schaffer labs. See the Retrograde AAV Inventory Page . Don’t See What You’re Looking For? ...viruses would be helpful to the scientific community. Return to top...

...filtering options. *AAVrg = retrograde serotype, produced with the AAV retro helper plasmid from the Karpova... dependent) Serotype AAV1 AAV2 AAV5 AAV8 AAV9 Retrograde AAV PHPeB Clear Filters ID Name Receptor and ...Karpova and Schaffer labs. See the Retrograde AAV Inventory Page . Browse all Chemogenetics AAV Don’t ...viruses would be helpful to the scientific community. Return to top...

...mammalian tissue culture cells, the R-mAbs are secreted into the culture media and can be collected for...domains to obtain recombinant hybridomas, which secrete antibodies as Fab' fragments, isotype-switched ...antibodies against zebrafish neural receptors and secreted proteins suitable for wholemount immunostaining...libraries. Immunol Lett. 2015 Oct;167(2):95-102. Joanna Bereta Sybody Generation Toolbox for making synthetic ...-rabbit") ID Plasmid Description Gene/Insert PI Return to top Do you have suggestions for other plasmids...

... zebrafish cell surface receptors and secreted proteins. Return to top Other Resources Addgene Blog Posts...described below . ID Plasmid Description Gene/Insert PI Return to top Highlights of Zebrafish Tools in our Collection...-binary expression system for use in zebrafish - Bret Pearson Lab MAZe: a tool for mosaic analysis of ...for analyzing single cell genomics in zebrafish. Return to top Do you have suggestions for other plasmids...

...Browse all Control AAV *AAVrg = retrograde serotype, produced with the AAV retro helper plasmid from the Karpova...serotypes. Serotype AAV1 AAV2 AAV5 AAV8 AAV9 AAVrh10 Retrograde AAV PHP.eB PHP.S PHP.V1 CAP-B10 CAP-B22 MaCPNS1...Karpova and Schaffer labs. See the Retrograde AAV Inventory Page . Don’t See What You’re Looking For? ...viruses would be helpful to the scientific community. Return to top...

...continually growing. ID Plasmid Description Industry PI Return to top Other Coronavirus Plasmids: SARS-CoV, MERS...materials are available only in the US at this time. Return to top Plasmids Encoding Mammalian Genes or Inserts...related plasmids see our Immunology Resource Guide . Return to top General Plasmid Tools SHERLOCK and DETECTR...Transcriptase plasmids from Andrew Ellington's lab. High-surety isothermal amplification and detection of SARS-...lab to ask them to deposit the plasmid with us. Return to top Pooled Libraries SARS-CoV-2 Spike (S) Ectodomain...

...interest by fusing it to a fluorescent protein. FRET Find FRET pairs and standards to study protein-protein... empty backbones. Fluorescent Protein Kits cpFRET Kit: FRET based biosensors - Pertz Fluorescent Protein...

...HIV-based lentiviral particles Murine leukemia-based retroviral particles Vesicular stomatitis virus particles...Lentiviral Envelope and Packaging Plasmids Popular γ-Retrovirus Envelope and Packaging Plasmids Reporter Plasmids...vector expressing firefly luciferase. pCMV-FLuc - Retroviral reporter vector expressing firefly luciferase...Resources Ginkgo Bioworks COVID-19 Plasmid Collection Return to top Do you have suggestions for other plasmids...

...viral service encoding tools for monosynpatic retrograde neuronal tracing, to be used with modified rabies...be used with modified rabies virus to perform retrograde neuronal tracing. Note : Addgene does not distribute...deletion-mutant rabies virus AAV1 Wickersham 100799 pAAV-TREtight-mTagBFP2-B19G AAV1 Wickersham Introduction to ..., Finke S, Conzelmann KK, Callaway EM. 2007a. Retrograde neuronal tracing with a deletion-mutant rabies...

...filtering options. *AAVrg = retrograde serotype, produced with the AAV retro helper plasmid from the Karpova... dependent) Serotype AAV1 AAV2 AAV5 AAV8 AAV9 Retrograde AAV PHPeB Clear Filters ID Name Promoter Sensor...Karpova and Schaffer labs. See the Retrograde AAV Inventory Page . Don’t See What You’re Looking For? ...viruses would be helpful to the scientific community. Return to top...

..., or by suggesting plasmids for the collection. Return to top Plasmid Kits for Microbiology Kits are sets...therefore supplied as one item. Fluorescent Proteins FRET-based biosensors : E. coli expression - Pertz Lab...common S. cerevisiae lab strains - Ralser Lab Return to top Resources for Microbiology General CRISPR...Genome Database (SGD) (Link opens in a new window) Return to top Do you have suggestions for other plasmids...

... genome editing logistics - Jose-Antonio Daros. Return to top Empty Backbones The table below highlights... Plasmid Description Promoter Cloning Method PI Return to top Fluorescent Proteins The table below highlights...protein of choice. ID Plasmid Description Promoter PI Return to top Other Resources Addgene Blog Posts RUBY-...Arabidopsis thaliana based on RNA-seq analysis. Return to top Do you have suggestions for other plasmids...