We narrowed to 93 results for: SAB;
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TypeBlog Post... proteins (FPs) have become one of the most indispensable tools in modern biological research. Any microscopist...
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Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish
TypeBlog Post...stroke and the third leading cause of death and disability worldwide. An ischemic stroke occurs when arteries... -
CRISPR Plasmids - Empty gRNA Vectors
TypeCollection...Mammalian/Lentiviral See paper none S. pyogenes Blast Sabatini and Lander lentiCRISPR v2 52961 Mammalian/Lentiviral...Mammalian/Lentiviral hU6 none S. pyogenes Puro Sabatini sgBbsI (p2Tol-U6-2xBbsI-sgRNA-HygR) 71485 Mammalian... -
Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)
TypeBlog Post... and incomplete PCR methods yield a minority of usable product with two 5′ overhangs, so these approaches... -
Antibodies 101: Buffers, Storage, and Conjugates
TypeBlog Post...below, you’ll likely be able to create a small, usable panel. First, fluorophores vary in brightness.... -
Luminescent Imaging with Nano-lanterns
TypeBlog Post...Fluorescent imaging techniques have become indispensable tools for molecular and cell biologists over... -
15 Years of Addgene: The Top 15 Plasmids
TypeBlog Post...pRPE. scramble shRNA - This plasmid from David Sabatini’s lab is a 3rd generation lentiviral vector that... -
Plasmids 101: Screens vs. Selections
TypeBlog Post... and resources References Wang, T., Wei, J.J., Sabatini, D.M., Lander, E.S. Genetic screens in human cells... -
CRISPR Guide
TypeCollection...them referred to as INTEGRATE ( I nsertion of T ransposable E lements by G uide R NA- A ssisted T argeting... 661–667. PMID: 25961408 Wang, T., Wei, J. J., Sabatini, D. M., & Lander, E. S. (2014). Genetic screens...Livny, J., Regev, A., Koonin, E. V., Hung, D. T., Sabeti, P. C., Collins, J. J., & Zhang, F. (2017). Nucleic... -
All in a Twist: dsRNA
TypeBlog Post...sources of dsRNA Cellular sources of dsRNA Transposable elements such as long/short interspersed nuclear... -
Supporting Rare Disease Research with the 2020 BeHEARD Awards
TypeBlog Post... CTD is the second cause of male intellectual disability after Fragile X. There is no cure for CTD, which... -
Choosing the B(right)est Fluorescent Protein: Photostability
TypeBlog Post...fluorophores perform in ‘real experiments’, it is advisable to perform time-lapse imaging of cells producing... -
Developing Lab Management Software for Biology
TypeBlog Post...permanent, like a tray or a freezer. Some are disposable, like a plastic tube. Talk with your lab team... -
New Tool for Lineage Tracing: The ClonTracer Library
TypeBlog Post...Resources on the Addgene Blog Learn about the Sabatini and Wood Drug Screening Kit Find Out How to Best... -
CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9
TypeBlog Post...system is often used to knock-out, or permanently disable, protein-coding genes by generating insertions ... -
Plasmids 101: The Wide World of Natural Plasmids
TypeBlog Post...antibiotic resistance-mediated selection is indispensable for cloning work, antibiotics and plasmid-encoded... -
Antibodies 101: Stripping and Reprobing Western Blots
TypeBlog Post... the membrane, making that section of the blot unusable for reprobing. Preparing your western blot Run... -
CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems
TypeBlog Post... E, Bondy-Denomy J, Corn JE, Doudna JA (2017) Disabling Cas9 by an anti-CRISPR DNA mimic. Sci Adv 3:e1701620... -
Adeno Associated Virus (AAV) for Cell and Gene Therapy
TypeBlog Post...immunogenic or carcinogenic profiles have limited their usability in clinical settings. On the other hand, vectors... -
Viral Vectors 101: Producing Your rAAV
TypeBlog Post...decontamination procedures (Korte et al., 2021) for all disposable items and surfaces that come into contact with...