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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 236 results for: SPL

Showing: 41 - 60 of 236 results

  1. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ...antibodies (rAbs) either produced de novo using yeast display technology or produced from hybridoma antibodies...The 11 IPI synthetic rAbs, developed using yeast display technology, are directed to integrins that bind...fragments (Fabs) that bound an amino acid region displayed by a subset of the RGD-binding integrins containing...Synthetic integrin antibodies discovered by yeast display reveal αV subunit pairing preferences with β subunits...

  2. Bacterial Expression Systems

    Type
    Collection
    ...proteins of interest are fused to each part of a split FP. When the candidate proteins interact, the pieces...reconstruct the full FP. Find plasmids containing FPs and split FPs that can be fused to your proteins of interest...Makes Visualizing Protein–Protein Interactions Easy Split Fluorescent Proteins for Studying Protein–Protein...

  3. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    ...proteins (PA-FPs) are fluorescent proteins that display unique changes in their spectral properties upon...population that is more neutral, causing PA-GFP to display almost no fluorescence when illuminated with 488nm...fact, many of the early reversible PA-FPs also display this “negative” photoswitching. In 2008, scientists...than mEos2 (mEos4b is completely monomeric) and display bright and photostable green and red states. Beyond...

  4. Visualizing Your Plasmid Request Data

    Type
    Blog Post
    ... a wide audience. The dashboard that I made to display the data in the CSV file is open for anyone to ...Addgene Requests Dashboard   The dashboard will display the following items (Fig. 1): A row with four... the CSV is shown (figure 2) and the dashboard displays the data in a standardized way. Since Addgene ...

  6. AAV Q&A with Tim Miles

    Type
    Blog Post
    ...potential strategy is to use split-inteins to mediate trans-protein splicing. Note, however, that this will...scenario, you may be best served by a dual vector, split intein approach.   Q3: What’s the best method for...

  7. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    ...(AAV). The components of these vectors are also split across several plasmids (often three), for safety...virus must assemble and have appropriate factors displayed on the outside of the particle to interact with...components needed for viral packaging, assembly, and display. Pol, the last component, contains the reverse ...

  10. Fluorescent Protein Guide: In Vivo Imaging

    Type
    Collection
    ...development of the original iRFP has resulted in a split fluorescence complementation probe used for detecting...690/713 6.2 piRFP iRFP720 702/720 5.8 piRFP720-N1 iSplit 690/713 5.3 pPAS-E and pK-GAFm Photoactivatable...

  12. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    ...talking to themselves as they compare the features displayed on the model with their mental model of the protein...primed for cleavage. This structure captures the displaced non-target DNA strand and shows the HNH nuclease...target DNA strand. In addition, this model also displays the several Lys/Ala mutations reported by Ian ...

  13. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    ...each sample. Successfully analyzed samples will display the following parameters: Sample - The unique ...analysis can be sorted by any of the parameters displayed in the summary table. You can also use your browser..., you’ll find an Indel plot (left panel) which displays the inferred distribution of indel sizes in the...

  14. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ...sequence NGG compared to wild-type SpCas9, which also displays some cleavage with an NGA PAM. This variant may... AAV delivery. NmCas9, another small ortholog, displays lower off-target editing than wild-type SpCas9...rather than the blunt ends created by Cas9. Cas12a  displays lower off-target editing than SpCas9 (Kim et al...

  15. AAV for Neuronal Tracing

    Type
    Collection
    ...Name Description Serotype PI 52473 pAAV-synP-FLEX-splitTVA-EGFP-B19G Can be used to complement deletion-mutant...rabies virus AAV1 Wickersham 100798 pAAV-syn-FLEX-splitTVA-EGFP-tTA These AAV together can be used to complement...

  16. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    ...developed into a split aptamer that can be used to investigate RNA assembly. The split aptamer is composed...References Alam, Khalid K., et al. "A fluorescent split aptamer for visualizing RNA–RNA assembly in vivo...

  17. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    ...for DNA repair synthesis. The ssDNA strand then displaces one strand of the homologous DNA donor and can...formation of a DNA structure referred to as the displacement loop (D loop). The recombination intermediates...synthesized portion of the invasive strand is displaced from the template and returned to the processed...

  18. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ...abundant and ethanol concentrations are low, pADH1 displays low promoter activity (~20% activity of the strong...complexity of the system, researchers have used split GAL4 architecture or combined the system with Flp...Valle Rodriguez, Alberto, Dominic Didiano & Claude Desplan. “Power tools for gene expression and clonal analysis...

  19. p53 Pathway

    Type
    Collection
    ...PubMed PMID: 24739573 . Uncovering the role of p53 splice variants in human malignancy: a clinical perspective.... PubMed PMID: 12619118 . p53 mutant mice that display early ageing-associated phenotypes. Tyner SD, Venkatachalam...

  20. CRISPR Guide

    Type
    Collection
    ... reverse transcription. The edited DNA strand displaces the unedited 5’ flap, and the resulting heteroduplex...including editors based on a second ADAR variant that displays improved specificity, and editors carrying an ... in vivo RNA imaging or to control alternative splicing. Figure 13: Overview of RNA targeting (A) and ...locus-specific targeting of long RNAs with CRISPR-Display. Nature Methods , 12 (7), 664–670. PMID: 26030444...
Showing: 41 - 60 of 236 results