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We narrowed to 238 results for: SPL

Showing: 41 - 60 of 238 results

  1. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

    Type
    Blog Post
    ...detecting protein-protein interactions that uses split fluorescent proteins tethered to potential protein...visualize PPIs. This fluorescent protein (FP) is split into two fragments that individually lack fluorescence...fluorescence microscopy. Figure 1: How BiFC works. A split FP (e.g., yellow fluorescent protein, YFP) is reunited...information about the structures of the tested PIPs. Many split FPs have been validated for BiFC (Kodama and Hu,... Hu, 2012). Such controls are necessary because split FPs can associate, albeit at relatively low frequencies...

  2. Rett Syndrome

    Type
    Collection
    ...RNA editing, gene replacement therapy, RNA trans-splicing, and protein replacement to reverse the impacts...2002. Insight into Rett syndrome: MeCP2 levels display tissue- and cell-specific differences and correlate...

  3. Hot Plasmids: Fall 2025

    Type
    Blog Post
    ...manipulation transplantation (AMT), CRISPR-modified adipocytes (fat cells) are transplanted as a mass that... proposed, researchers have shown that these transplanted mitochondria can exert their powers for better...upon Ca2+ binding. Most importantly, it doesn’t display the problematic photoswitching upon blue illumination...donor-derived CRISPRa-AAV-treated WAT cells were transplanted into the vicinity of any one of five(!) different...

  4. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...Bacterial vector for Myc tag pETcon(-) - Yeast surface display vector with a C-terminal Myc tag pPMW-attB - pUASp...expression (Gateway) pYD1 - Expression, secretion, and display of proteins on the extracellular surface of S. ...

  5. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    ...anchoring domains The original iGluSnFR in the pMinDisplay backbone is fused to a specific peptide segment...TMD) that anchors the sensor to the membrane and displays it on the extracellular side. In addition to the... 4 Sequence schematics for PDGFR, GPI, and SGZ display constructs. Image adapted from Aggarwal et al… ...transmembrane domain in the mammalian expression pMinDisplay vector. GPI contains a C-terminal glycosylphostidylinositol...

  6. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

    Type
    Blog Post
    ...array targeting the MAPT exon 10 splice acceptor and two putative splice enhancers. After AAV-mediated delivery...previously characterized Cas13 enzymes, CasRx also displays very high on-target efficiency; where shRNA treatment...produced 500-900 significant off-targets, CasRx displayed zero. Unlike Cas9, for which efficiency varies...

  7. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ...following a G more efficiently than BE3, but APOBEC3G displayed less predictable sequence preferences.  The Liu...editors (ABEs). ABE7.10 is the most active editor, displaying an average editing efficiency of 53% with an ...of target positions 4-7. ABEs 6.3, 7.8, and 7.9 display slightly wider editing windows of position 4-9,...produce a mixed population of edits, ABEs do not display significant A to non-G conversion at target loci...Highlights Komor et al., 2016 BE1, BE2, BE3   BE3 displays highest editing efficiency but higher indel formation...

  8. Pooled Library Amplifications

    Type
    Blog Post
    ...from the Mark Dawson lab Re-transformation of the SPLINTR Libraries Protocol.   Selecting your strain...perform several individual transformations, such as splitting 400 ng of DNA in 100 μL of cells over four individual...Electroporation Protocol (DOCX, 15.8 KB) Barcode SPLINTR Pooled Libraries 200,000–1,200,000 60 bp ... 200 ng Liquid Re-transformation of the SPLINTR Libraries (PDF, 401 KB) Screening Zhang Lab...

  9. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ...can be found at Addgene. Split FPs – some FPs (e.g. GFP, Venus) can be split into two halves, which are...proximity, they will form the full FP and fluoresce. Split FPs can be used to determine the proximity of two...two proteins fused to the halves of the split FP. This technique is also is also called Bimolecular Fluorescence...

  10. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ...fusion proteins for imaging, tracking, modulating splicing, and regulating expression of specifically targeted...described in bacterial cells, LwaCas13a does not display nonspecific RNA cleavage activity in eukaryotic... LwaCas13a to shRNA, they found that LwaCas13a displays similar knockdown efficiency but no significant...to see this group of enzymes continue to make a splash in the CRISPR field. We feel that the culture of...

  11. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ...antibodies (rAbs) either produced de novo using yeast display technology or produced from hybridoma antibodies...The 11 IPI synthetic rAbs, developed using yeast display technology, are directed to integrins that bind...fragments (Fabs) that bound an amino acid region displayed by a subset of the RGD-binding integrins containing...Synthetic integrin antibodies discovered by yeast display reveal αV subunit pairing preferences with β subunits...

  12. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    ... of interest do not lie beyond the dimensions displayed in the plot. (B) A more restrictive gate results... second measurement to choose for graphically displaying your events. You are free to choose any size-... the positioning and shape of the populations displayed. As the separation pattern largely stays the same... cells but not dendritic cells. Using a graph displaying both stains, I can quickly separate T killer ...

  13. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    ...proteins (PA-FPs) are fluorescent proteins that display unique changes in their spectral properties upon...population that is more neutral, causing PA-GFP to display almost no fluorescence when illuminated with 488nm...fact, many of the early reversible PA-FPs also display this “negative” photoswitching. In 2008, scientists...than mEos2 (mEos4b is completely monomeric) and display bright and photostable green and red states. Beyond...

  14. Visualizing Your Plasmid Request Data

    Type
    Blog Post
    ... a wide audience. The dashboard that I made to display the data in the CSV file is open for anyone to ...Addgene Requests Dashboard   The dashboard will display the following items (Fig. 1): A row with four... the CSV is shown (figure 2) and the dashboard displays the data in a standardized way. Since Addgene ...

  15. AAV Q&A with Tim Miles

    Type
    Blog Post
    ...potential strategy is to use split-inteins to mediate trans-protein splicing. Note, however, that this will...scenario, you may be best served by a dual vector, split intein approach.   Q3: What’s the best method for...

  18. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    ...(AAV). The components of these vectors are also split across several plasmids (often three), for safety...virus must assemble and have appropriate factors displayed on the outside of the particle to interact with...components needed for viral packaging, assembly, and display. Pol, the last component, contains the reverse ...

  19. Brain Initiative Collection

    Type
    Collection
    ...promoter Viviana Gradinaru 100798-AAV1 pAAV-syn-FLEX-splitTVA-EGFP-tTA helper virus for monosynaptic tracing...monosynaptic tracing; to be coinjected with pAAV-syn-FLEX-splitTVA-EGFP-tTA Ian Wickersham 104052-AAVPHP.V1 pAAV-...
Showing: 41 - 60 of 238 results