We narrowed to 402 results for: SUA

...neuroscience research. The use of genetic tools for visualizing and perturbing circuits together with the development...expressed. As these epitopes tags have no intrinsic visual signal, they cannot be detected directly in live...also use multiple FPs in single experiments and visualize numerous molecules or structures at once (Shaner...multi-color imaging experiments, the second color is usually provided by red fluorescent proteins such as tdTomato...using smFPs and standard neuronal tracers. 2. Visualization of neuronal sub-cellular structures GFP is limited...structures. For instance, smFPs have been used to visualize “thorny excrescence” (TE) spines in the Hippocampal...appear to be very effective probes for finely visualising sub-cellular structures or low-abundance proteins...

...to high Uses visuals, audios, and text. Incredibly immersive and flexibility to visualize and contextualize...often more suited for cartoons or graphics for visuals. However, it’s much more challenging to design ...from smartphones with low data requirements.  No visuals. Variety of hosting platforms can splinter audience... digitally and physically. Easy to incorporate visuals if needed. Traditional media formats are quite ...  Photography Medium (high for microscopy) Visually compelling. Easy to convey a large number of details...contextualize. Graphics/cartoons (no words) Low Visually compelling and flexible. Can be used to illustrate...

...non-model organisms. Therefore, transient methods are usually the first choice for investigating gene function...In the plant sciences “transient transformation” usual means that neither a stable cell line or plant is... re-invent the wheel. Test different leaf ages Usually younger leaves work better. Not every leaf is equally...with histochemical GUS-staining is a very useful visual reporter (Figure 2). The GUS enzyme cleaves a substrate...which will then form a blue precipitate. GUS is usually the first choice for establishing a new transformation...substrate to produce bioluminescence. Luciferases usually have shorter mRNA and protein half-lives then GUS.... Syringe infiltration (D'Aoust et al., 2009): Usually a syringe without a needle is used to inject an...

...sound to visualize bacteria in the lab, I was excited to learn about creative approaches to visualize bacteria...microbes through a microscope is not the only way to visualize them. You can also “hear” them. Addgene depositor... as bacterial expression of dyes allows dye visualization in vivo using ultrasound (for an example see...showcased complex connections and ideas in various visualized approaches from hand-drawn comics (Dr. Sanja ...interesting and approachable!   The grand finale of visual impressions was delivered by the ASM Agar Art Winners...

...ELISA or Western Blotting, but it allows you to visualize a target of interest within an intact cell, tissue...these terms refer to the use of antibodies to visualize a target within an intact sample, a cell in the... case of IHC. IF is used specifically when we visualize the antibodies using fluorescence (for some background... also refer to assays using non-fluorescent visualization methods. Stay tuned for a future blog post to...direct IF, researchers use a single antibody to visualize their target. These antibodies are generated against...fluorescent molecule allowing one to “directly” visualize their target. In contrast, indirect IF requires...antibodies conjugated to distinct fluorophores to visualize multiple targets at once. An advantage of multiplexing...

...the insert ended up in the direction you want - usually by Sanger sequencing or further restriction digests... use a voltage difference across a gel matrix (usually agarose) to pull your negatively charged DNA through... individually. There are a variety of ways to visualize the DNA in your gel (this table is not inclusive...all gel stains):  Stain Pre or Post Run? Visualization Sensitivity (ng DNA) SYBR Safe Pre and Post...respectively). Some of the above stains require you to visualize your DNA using UV light – please note that UV ...personal protective equipment should be worn when visualizing using UV as it can cause damage to the eyes and...backbone alone plate). Unsuccessful ligations will usually result in few colonies on both plates (unsuccessful...

...organism's genome, for example. Amplification is usually necessary to produce sufficient quantities of library...the shared plasmid backbone a single time and visualize that digestion on an agarose gel ( see restriction...all liquid has been absorbed by the agar. This usually takes 1-2 minutes. Critical Be careful not to rip...

...the dynamic or working range of a given reagent (usually an antibody).      Figure 1: A protein ...number of helpful suggestions.  Gels Gel issues usually come down to voltage and time. Make sure to load...seeing a smiley face at an appropriate voltage (usually 10–15 V/cm of gel), your gel may be overheating...protein tag or an IgG binding site, allowing you to visualize them with a conjugated antibody. If you do not...with 20% methanol, and place over a light box to visualize proteins after transfer. Troubleshooting The ...with specific blockers, and the manufacturer will usually include recommended blocking conditions on the ... antibodies, the recommended dilution range is usually between 1:5,000 and 1:200,000. Check the manufacturer...

...studies, which are evinced by the Tet system and visualization of single molecules via targeted fluorescent...2013 August 20th; 14(1). TRICK: A method for visualizing the first round of translation in living cells...anticipate that this tool can also be used to visualize translocations, genome rearrangements and cancer-associated...GFP, BFP and mCherry), the authors were able to visualize simultaneously several parts of the genome and...variety of experiments including subcellular visualization of 6 different organelles, a cell cycle indicator...proteins have been used as subcellular markers to visualize the dynamics and interactions between the endoplasmic...These fusion proteins can act as markers for visualization of subcellular structure, for performing live-cell...

...marker, studying protein-protein interactions, visualizing promoter activity, and much more. Read on to ...C-terminus of a protein, which allows the scientist to visualize when and where the gene is expressed. Click here...cells in order to understand their activity and visualize the effects of alterations to this activity. Learn...put under control of promoters of interest, to visualize the developmental stage at which these promoters...particular cell populations in drug screens, to visualize micrometastases in nude mice in cancer studies...to label pathogenic intracellular microbes to visualize host/pathogen interactions.   Do you have a favorite...

... Because the G is only one base, the Zhang lab usually adds it when they order the oligo. If your spacer...single-allelic and bi-allelic cells. Single-allelic cells usually make up the majority in culture unless the targeting...Ran et al. (2013). As for target selection, we usually pick 3–6 guides around the region to find the most...consider using ssDNA oligo as donor template, it's usually more efficient than plasmid donor. Typical design...CRISPR protospacer target in the HR template, you usually would need to make mutations of the HR template...probably a single stranded DNA (ssDNA) oligo which usually work better than plasmids. For ssDNA oligo design...PCR reaction. The gDNA extracted in this way is usually more concentrated than using other kits like the...

...markers found within the cytosol/nucleus, or visualizing DNA instead of proteins. Some stains can diffuse...antibodies used for target marker detection are usually conjugated to a fluorophore, not every marker will...bead-activated cell sorting, or MACS. But MACS usually only allows for sorting based on a single (lineage...proliferation between cell populations can be visualized, achieved through, for example, a previously ...divisions).   Figure 4: Proliferation dyes can visualize proliferative capacity and speed of cells. (a)...hopefully learned a few new methods of target visualization beyond the simple surface labeling. Let’s briefly...

... fluorescence is no longer quenched and can be visually detected using lateral flow strips. Since the ...AIOD-CRISPR) assay – for rapid, ultrasensitive and visual detection of novel coronavirus SARS-CoV-2 Sensitivity...machines by DIY-Bio, P51 cardboard fluorescence visualizer with 9V battery Approach :This preprint uses ...cleavage. This cleavage is coupled to fluorescence visualization. In this technique, however, instead of using...the authors employ a P51 cardboard fluorescence visualizer that is powered by a 9V battery which uses a ... Upon binding, changes in fluorescence can be visualised. Another approach was to apply the same concept...

...digestion (usually 3-6bp) Restriction Site: Your chosen restriction site for cloning (usually 6-8bp) ...primer that binds to the sequence to be amplified (usually 18-21bp) When selecting restriction sites, you...the desired location in your recipient plasmid (usually in the Multiple Cloning Site (MCS)), but do not...genomic DNA or a cDNA library), roughly 18-21bp is usually sufficient to give specificity and to also be compatible...

...wavelength of light. The switch or photoconversion usually takes less than a second. The first successful ...the fluorescence the better. More fluorescence usually means more photons are being emitted, making it...studying organelle trafficking. Monomeric PA-FPs are usually preferred for studying single protein characteristics...and after photoactivation. A high contrast ratio usually indicates that a PA-FP has a low level of spontaneous...protein (mito-PAGFP) the Youle Lab was able to visualize and quantify the fusion dynamics of mitochondria... best PA-FPs used for super-resolution imaging usually have the following characteristics-high contrast...

...basic observation and dissections. Students don’t usually perform more modern techniques used in genetics...Whole mount in Situ Hybridization (WISH) for visualizing expression of genes. Double-stranded RNA (dsRNA...protocol resources. Find Sánchez lab Plasmids Visualizing gene expression via Whole Mount In Situ Hybridization...sample, several washes, and temperature changes to visualize the expression of specific genes. After riboprobe...of T7 polymerase encoded in the E. coli genome (usually controlled by IPTG). For this IPTG-induced expression...

...organisms. Furthermore, fusing FPs to another protein usually does not affect its fluorescence. Therefore, FPs...measuring the fluorescence intensity); and 2) to visualize the localization of the FP (fused to the protein...emission color (emission wavelength range) FPs are usually classified by emission color as outlined below ...Therefore, it is recommended to use monomeric FPs (usually denoted by a “m” as the first letter in the protein...Resources: FP guide at Addgene Interactive Visualization of Fluorescent Protein Properties  Fluorescence...

...gives off a signal read by the machine’s sensors. (Usually this something is a cell, but it could be debris...populations are positive for both markers, because we usually assume (0,0) to be at the intersection of X,Y. ...the researcher, based on their controls. You can usually tell where this point is by looking at the different...plot generated in Biorender! While a helpful visualization tool, a quadrant will often be too clean for...-Anacker et al., 2017, with quadrants to help visualize the different populations. Used under Creative...

...system useful for visualization of cellular extensions, such as neurons in the fly visual system, which has...endogenous fluorescence in live animals only permits visualization of two colors. Similarly, Flybow adapts Brainbow...pdb-prot5546. Richardson, Alexander, et al. "Visualizing the Fate of Transplanted K14-Confetti Corneal...Cell Deficiency." Investigative ophthalmology & visual science 59.3 (2018): 1630-1640. Sakaguchi, Richi...

...proceed. Usually a guanine or cytosine is used at the 3’ end, and the 5’ end of the primer usually has stretches...