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We narrowed to 420 results for: SUA

Showing: 41 - 60 of 420 results

  1. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    ...markers found within the cytosol/nucleus, or visualizing DNA instead of proteins. Some stains can diffuse...antibodies used for target marker detection are usually conjugated to a fluorophore, not every marker will...bead-activated cell sorting, or MACS. But MACS usually only allows for sorting based on a single (lineage...proliferation between cell populations can be visualized, achieved through, for example, a previously ...divisions).   Figure 4: Proliferation dyes can visualize proliferative capacity and speed of cells. (a)...hopefully learned a few new methods of target visualization beyond the simple surface labeling. Let’s briefly...

  2. Plasmid Cloning by PCR

    Type
    Blog Post
    ...digestion (usually 3-6bp) Restriction Site: Your chosen restriction site for cloning (usually 6-8bp) ...primer that binds to the sequence to be amplified (usually 18-21bp) When selecting restriction sites, you...the desired location in your recipient plasmid (usually in the Multiple Cloning Site (MCS)), but do not...genomic DNA or a cDNA library), roughly 18-21bp is usually sufficient to give specificity and to also be compatible...

  3. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    ...wavelength of light. The switch or photoconversion usually takes less than a second. The first successful ...the fluorescence the better. More fluorescence usually means more photons are being emitted, making it...studying organelle trafficking. Monomeric PA-FPs are usually preferred for studying single protein characteristics...and after photoactivation. A high contrast ratio usually indicates that a PA-FP has a low level of spontaneous...protein (mito-PAGFP) the Youle Lab was able to visualize and quantify the fusion dynamics of mitochondria... best PA-FPs used for super-resolution imaging usually have the following characteristics-high contrast...

  4. Educational Planarian Tools for Teaching Developmental Biology Techniques

    Type
    Blog Post
    ...basic observation and dissections. Students don’t usually perform more modern techniques used in genetics...Whole mount in Situ Hybridization (WISH) for visualizing expression of genes. Double-stranded RNA (dsRNA...protocol resources. Find Sánchez lab Plasmids Visualizing gene expression via Whole Mount In Situ Hybridization...sample, several washes, and temperature changes to visualize the expression of specific genes. After riboprobe...of T7 polymerase encoded in the E. coli genome (usually controlled by IPTG). For this IPTG-induced expression...

  5. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ...organisms. Furthermore, fusing FPs to another protein usually does not affect its fluorescence. Therefore, FPs...measuring the fluorescence intensity); and 2) to visualize the localization of the FP (fused to the protein...emission color (emission wavelength range) FPs are usually classified by emission color as outlined below ...Therefore, it is recommended to use monomeric FPs (usually denoted by a “m” as the first letter in the protein...Resources: FP guide at Addgene Interactive Visualization of Fluorescent Protein Properties  Fluorescence...

  6. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    ...gives off a signal read by the machine’s sensors. (Usually this something is a cell, but it could be debris...populations are positive for both markers, because we usually assume (0,0) to be at the intersection of X,Y. ...the researcher, based on their controls. You can usually tell where this point is by looking at the different...plot generated in Biorender! While a helpful visualization tool, a quadrant will often be too clean for...-Anacker et al., 2017, with quadrants to help visualize the different populations. Used under Creative...

  7. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

    Type
    Blog Post
    ...system useful for visualization of cellular extensions, such as neurons in the fly visual system, which has...endogenous fluorescence in live animals only permits visualization of two colors. Similarly, Flybow adapts Brainbow...pdb-prot5546. Richardson, Alexander, et al. "Visualizing the Fate of Transplanted K14-Confetti Corneal...Cell Deficiency." Investigative ophthalmology & visual science 59.3 (2018): 1630-1640. Sakaguchi, Richi...

  8. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ...membrane, stained with antibodies, and finally visualized.   This process allows you to detect a single... the proteins are immobilized onto a membrane, usually composed of a nitrocellulose or polyvinylidene ... Stuart Orkin's and Daniel Bauer's labs.    Visualizing your protein of interest with the help of secondary...secondary antibodies In order to visualize proteins on the membrane the antibodies are typically conjugated...species-specific secondary antibody is used to visualize the proteins. In this set-up, multiple secondary...

  9. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    ... cover some screening methods to identify them. Visual screening Regardless of the RNAi-trigger type you...As a plant researcher, the traditional hpRNA and visual screening method was limited in two substantial...labor-intensive processes to isolate RNAi plants. With a visual reporter of RNAi, phenotypes, such as morphological...specific target required physical isolation of the visually identified subpopulation. For traditional cell... analysis. Instead, microscopic plants must be visually identified as undergoing RNAi through the fluorescent...

  10. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    ...protein is targeted with an antibody, you can visualize the protein via fluorescence or chemiluminescence...and tissue labeling Antibodies can be used to visualize tissues (immunohistochemistry) or cells (immunocytochemistry...preserved, this technique allows you to not only visualize the presence of specific proteins, but their localization...contextualize its localization. These could be visualized using fluorophore-conjugated antibodies (i.e.... using an unconjugated primary antibody, then visualized using a fluorophore-conjugated secondary antibody...

  11. Protocol - How to Design Primers

    Type
    Protocol
    ...proceed. Usually a guanine or cytosine is used at the 3’ end, and the 5’ end of the primer usually has stretches...

  12. Antibodies 101: Affinity Tags

    Type
    Blog Post
    ...for direct visualization while epitope tags are used for both direct and indirect visualization. Some of...alternative to amylose resin and also provides a visual detection mechanism via applications such as western...affinity tags can sometimes be repurposed for visualization. However, due to the nature of the size, structure...

  13. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    ... has been incorporated into the host organism, usually achieved with the proper selection marker or antibiotic... fluorescent fusion protein will allow you to visualize YGOI in vivo (try pcDNA3-EGFP). If you can get...various markers and promoters. If you need to visualize YGOI on a western blot or under a microscope, ... Pick your promoter, selection cassette and visualization vehicle to conduct your custom experiment. Fly...

  14. Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science

    Type
    Blog Post
    ...The first read At the first read of the paper, I usually try to grasp the main message of the work. I underscore...the given context. After this first read, I am usually able to construct the beginning of my review - ...another day - preferably the following morning - as usually deadlines are pressing. During this second read... (3) development of tools for data analysis/visualization using R/Shiny. She is passionate about open ...

  15. CRISPR Plasmids - dCas9-FokI

    Type
    Collection
    ...Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty...

  16. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type
    Blog Post
    ... then its concentration is detected through visualization of an enzymatic reaction. Types of ELISA There...its substrate is added and the reaction can be visualized via a colorimetric reaction. The direct ELISA...secondary antibody is conjugated to an enzyme for visualization. The indirect ELISA is used for similar experiments...additional amplification step, which helps to visualize low-abundance proteins. Figure 4: Overview...

  17. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ...protein tag for visualization (Fig. 1b). While the probes target chromatin to visualize DNA, an editor ...fluorescent protein can still be included for visualization purposes, but the key component of the editing...result in depletion of the FP, which can be easily visualized.  The tool-kit consists of highly useful vectors...

  18. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    ...The ocean is full of striking visuals from fluorescent jellyfish, bioluminescent phytoplankton, and colorful...shorter history. In 1987, scientists described an unusual blue-colored protein from the jellyfish Cassiopea...methods like sequencing are more definitive, but visual identification is a good place to start before ...FRET References Blanquet RS, Phelan MA (1987) An unusual blue mesogleal protein from the mangrove jellyfish...

  19. CRISPR Plasmids - Epigenetics

    Type
    Collection
    ...Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty...
Showing: 41 - 60 of 420 results