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Showing: 41 - 60 of 165 results

  1. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    ...chemical fuel sources. Like an actin filament or a microtubule, R bodies are a polymer of many copies of monomeric...ectosymbionts of Euplotidium (Ciliophora) that contain microtubule-like structures are bacteria related to Verrucomicrobia...

  2. Sweating the Small Stuff: Details in the Lab

    Type
    Blog Post
    ...time needed to prep for an experiment (labeling tubes never takes longer than 5 minutes, right?) As a ...   Visual Planning  Plate maps, pre-labeling tubes, and even drawing out experimental steps are worth...

  3. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    ...many different things do you simply refer to as a “tube”? We took steps towards solving this problem by...Prescribing an official name to all of our plates, tubes, and racks meant we had fewer errors in our LIMS...

  5. Plasmids 101: Modular Cloning

    Type
    Blog Post
    ...synthetic biologists to perform large scale, one-tube cloning reactions. In this blog, we’ll cover the...assemble multiple DNA fragments together in a one-tube reaction. Even though most MoClo reactions use Golden...

  7. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    ... to use multiple techniques for a particularly stubborn prep. Check the bacterial strain Increase the...desired density. The volume of your flask or culture tube should be at least four times greater than your ...

  8. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ...Antibodies may even aggregate just from dropping the tube they are contained in (Randolph et. al., 2018). ...aliquoting and storage process to prevent dropped tubes. Now that your antibody is produced, harvested, ...

  9. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    ..., mastered the art of finding how-to videos on YouTube long before he learned (somewhat successfully) ...                Two of the platforms we use at Addgene: YouTube, for longer educational videos, and Twitter, for...

  10. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    ...dilutions. Aliquot all of your reagents into single-use tubes and grab a fresh one for each set up.  Wipe down... al. "Detection of circulating Mycobacterium tuberculosis-specific DNA by droplet digital PCR for vaccine...

  11. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    ...templates. All PCRs are run as an 18 cycle, 25 μl single-tube reaction. If you are synthesizing a new DNA fragment...Watson, and Ingo H. Greger. "IVA cloning: a single-tube universal cloning system exploiting bacterial in...

  12. MXS Chaining

    Type
    Blog Post
    ...β-Actin Actin 4 Citrine 516nm/ 529nm human α-Tubulin Tubulin Repeatable, directional cloning   The ...

  13. Viral Production at Addgene

    Type
    Blog Post
    ...matching system that follows the sample from the DNA tube all the way to the cleared viral preparation pool...ensuring the identity of each sample from the DNA tube all the way to the viral preparation. After production...

  15. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ...components into plants. Nanoparticles (carbon nanotubes, Kwak et al., 2019), DNA nanostructures (Zhang... using chitosan-complexed single-walled carbon nanotube carriers. Nat Nanotechnol 14:447–455 . https:/...

  16. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...buffer called sheath fluid and transported through tubes or capillaries to a laser. As the cells move through...positive for a desired parameter is collected in a tube or plate, while a droplet containing a negative ...

  17. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    ...with all of the plasmids in the library in a single tube. The number of unique plasmids in a pooled library... submitted as 15 µL of DNA in a 1.5 mL microfuge tube at a concentration of 0.1–1 µg/µL (or bacterial ...

  19. New Antibody Animation!

    Type
    Blog Post
    ...found in mammals.      Subscribe to Addgene's YouTube channel for more helpful animations, useful protocol...
Showing: 41 - 60 of 165 results