We narrowed to 173 results for: TUB

...synthetic biologists to perform large scale, one-tube cloning reactions. In this blog, we’ll cover the...assemble multiple DNA fragments together in a one-tube reaction. Even though most MoClo reactions use Golden...

...Markers : Antibodies targeting popular markers like tubulin or epitope tags like Myc, GFP, and more. Neuroscience...

...prescribed order. These fragments can be pooled in one tube and ligated in as little as 30 minutes! Alternatively... per cloning reaction drops dramatically, as one tube of enzyme should be enough for at least 50 cloning...

... to use multiple techniques for a particularly stubborn prep. Check the bacterial strain Increase the...desired density. The volume of your flask or culture tube should be at least four times greater than your ...

...Antibodies may even aggregate just from dropping the tube they are contained in (Randolph et. al., 2018). ...aliquoting and storage process to prevent dropped tubes. Now that your antibody is produced, harvested, ...

..., mastered the art of finding how-to videos on YouTube long before he learned (somewhat successfully) ...                Two of the platforms we use at Addgene: YouTube, for longer educational videos, and Twitter, for...

...dilutions. Aliquot all of your reagents into single-use tubes and grab a fresh one for each set up.  Wipe down... al. "Detection of circulating Mycobacterium tuberculosis-specific DNA by droplet digital PCR for vaccine...

...β-Actin Actin 4 Citrine 516nm/ 529nm human α-Tubulin Tubulin Repeatable, directional cloning   The ...

...templates. All PCRs are run as an 18 cycle, 25 μl single-tube reaction. If you are synthesizing a new DNA fragment...Watson, and Ingo H. Greger. "IVA cloning: a single-tube universal cloning system exploiting bacterial in...

...matching system that follows the sample from the DNA tube all the way to the cleared viral preparation pool...ensuring the identity of each sample from the DNA tube all the way to the viral preparation. After production...

...Therefore, the localization of critical fusions (e.g. tubulin or connexin) has been used as a proxy for dimerization...Lifeact-mTurquoise2 (Plasmid #36201) or mScarlet-tubulin (Plasmid #85045). We often use restriction enzymes...

...components into plants. Nanoparticles (carbon nanotubes, Kwak et al., 2019), DNA nanostructures (Zhang... using chitosan-complexed single-walled carbon nanotube carriers. Nat Nanotechnol 14:447–455 . https:/...

...Ribosomal protein S6 kinase B1 TSC1/2 TSC1 TSC2 Tuberous sclerosis VHL Von Hippel-Lindau tumor suppressor...

...3 Siah Siah E3 ubiquitin protein ligase 1 TSC2 Tuberous sclerosis 2 TSP1 Thrombospondin 1 Return to top...

...Cre-dependent Cre-dependent expression of human 2N4R microtubule-associated protein tau with P301L mutation. 1...

...Plasmids Viral Vectors 101 eBook Viral Vectors 101 Youtube Videos Addgene's Biosafety Resources Addgene Protocols...

...buffer called sheath fluid and transported through tubes or capillaries to a laser. As the cells move through...positive for a desired parameter is collected in a tube or plate, while a droplet containing a negative ...

...plastic in spin columns and buffer bottles Waste tubes are made of 100% post-consumer recycled plastic ...buffers and columns sold separately Some, waste tubes sold separately No Other Minimized amount...

...with all of the plasmids in the library in a single tube. The number of unique plasmids in a pooled library... submitted as 15 µL of DNA in a 1.5 mL microfuge tube at a concentration of 0.1–1 µg/µL (or bacterial ...

...matching system that follows the sample from the DNA tube all the way to the cleared viral preparation pool...