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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 48 results for: Tight TRE

Showing: 41 - 48 of 48 results

  2. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    ...curing time. pCRISPomyces for editing in Streptomyces  Streptomyces bacteria produce a wide variety of bioactive...requires only 2-3 hours incubation. E. coli and T. citrea scarless editing plasmids Sheng Yang’s lab describes..., the system was used successfully in Tatumella citrea, another species of Enterobacteriaceae, without...created two “pCRISPomyces” systems for use in Streptomyces (Cobb et al., 2015). pCRISPomyces-1 includes...Gibson Assembly or restriction enzyme cloning. Streptomyces bacteria are more recombinogenic than E. coli... repression in the Gram-positive bacterium, Streptococcus pneumoniae. Robert Husson lab's M. tuberculosis...enzyme. They identified the Cas9 orthologue from Streptococcus thermophilus, Cas9sth1 as a robust and highly...

  3. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    ...toxic or insoluble proteins Origami2 (DE3)** Streptomycin and Tetracycline Contains highly active thioredoxin...—the difference being that the latter provides tighter control of basal expression. What if I don't see...

  5. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...piggyBac™, the number of integration copies can be tightly controlled by titrating the transposase to transposon...piggyBac-ing models and new therapeutic strategies.,” Trends Biotechnol., vol. 33, no. 9, pp. 525–33, Sep. 2015...

  6. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ...identically to BE3, modifying a 3-5 base window 18 bases upstream of the PAM.  David Liu’s lab generated BE3 variants... window of only a few nucleotides, so they are tightly constrained by PAM availability in the genome. ...Edits 3-5 base window surrounding -18 position upstream of the PAM Kim et al., 2017 SaBE3, BE3 PAM variants...

  7. Promoters

    Type
    Guide
    ...Mammalian promoter from phospholycerate kinase gene TRE Inducible Tetracycline response element promoter ...base pairs long and are adjacent and typically upstream (5’) of the sense or coding strand of the transcribed...polymerase) to bind to a promoter. Transcription is tightly controlled in both bacteria and eukaryotes. Promoters...nucleotides, TATAAT. Proximal Promoter Further upstream from the core promoter you will find the proximal...promoter is found approximately 250 base pairs upstream from the TSS and it is the site where general ... region is called the distal promoter which is upstream of the proximal promoter. The distal promoter ... DNA sequences located at the -10 (10 bp 5' or upstream) and -35 positions from the transcription start...

  8. Antibody Guide

    Type
    Guide
    ...molecular tools used in the lab to specifically and tightly bind to other proteins. Antibodies, produced as...creates variety in the antibody allowing for their extreme specificity occurs in the B cell itself prior to...Monoclonal antibodies are necessary in cases where extreme specificity is important. Their epitope specificity...recognizes and binds to the protein of interest. Signal strength is determined by how many antibodies bind the ...isolate specific proteins or protein complexes for downstream analysis. They are powerful tools for understanding...Reverse the crosslink, isolate DNA, and perform downstream analysis. Special considerations One type of ...
Showing: 41 - 48 of 48 results