We narrowed to 69 results for: UNK

1. Antibodies 101: Stripping and Reprobing Western Blots

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   ...happened during your western and your results look… funky? Do you need to start a whole new blot? Never fear...

2. 5 Tips for Troubleshooting Viral Transductions

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   ...Anthony, Simon J., et al. "A strategy to estimate unknown viral diversity in mammals." MBio 4.5 (2013): e00598...

3. Fluorescent Tagging of Endogenous Genes with SapTrap

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   ... endogenous loci, shedding light on previously unknown protein functions. References Schwartz, Matthew...

4. Polymerase Chain Reaction Overview and Applications

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   ...starting concentration. The cycle in which the unknown DNA is detected compared to the standard curve ...

5. Degrading DNA with Cascade-Cas3

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   ...III with a Cas10 effector, and type IV with an unknown effector. Type I has subtypes A through G, with...

6. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

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   ...https://doi.org/10.1128/aem.00990-19 Liljeruhm, J., Funk, S. K., Tietscher, S., Edlund, A. D., Jamal, S.,...

7. Plasmids 101: Optimizing Plasmid Yields

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   ...low yields from a plasmid whose copy number is unknown, it is entirely possible that you are working with...

8. Droplet Digital PCR for AAV Quantitation

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   ...dilutions onto our ddPCR plate. As the titer is unknown, each dilution should fall within the range of ...

9. Tips for arabidopsis transformation

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   ... culture into a container that is wide enough to dunk your plant’s flowers inside. 50 mL falcon tubes ...

10. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

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    ...dark matter in the genome. There are countless unknown regions yet to explore. I hope that this brief ...

11. Plasmids 101: Protein tags

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    ...proteosomes to degrade what the cell might consider junk protein. Though there are a number of chemical and...

12. How to Write a Scientific Review Article

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    ... writing: initially I felt that I needed a huge chunk of time to sit down and get anything done, so unless...

13. New Tools Enable CRISPRa for Neuroscience Applications

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    ...sequenced. Guide RNAs targeting several known and some unknown regulators of neuronal differentiation were enriched...

14. CRISPR Activation: A Practical Guide

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    ...isoform(s) common to a particular cell type are unknown or not readily available. By activating from the...

15. Tips for CRISPR Gene Editing in Mice

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    ...why some gRNA’s work better than others remain unknown, but fret not! The key to saving time and energy...

16. Transferable Skills Guide: Time Management

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    ...broken down time management into three conceptual chunks: prioritization, planning, and procrastination....

17. Multiple Plasmids at a Low Price: Kits from Addgene

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    ... pull-down procedure for the identification of unknown GEFs for small GTPases." Small GTPases 7.2 (2016...

18. Using Phosphoserine to Study Protein Phosphorylation

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    ...phosphatase treatment. If a candidate phosphatase is unknown, CIP or other generic phosphatases may dephosphorylate...

19. Viral Vectors 101: Optogenetic Tools

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    ...particular receptor or when a signaling pathway is unknown.      Figure 3: Chimeric OptoXR incorporating...

20. Finding Your Science Policy Path

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    ...science policy career was much more circuitous and unknown. In fact, in less than two years of walking away...