We narrowed to 81 results for: USP

1. Experimenting in an Open Source lab: from CRISPR to Cats

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   ...the Victoria area, please stop by. And if not, I suspect you can find a makerspace somewhere near you. The...

2. Degrading DNA with Cascade-Cas3

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   ... would reel in a big catch. It was originally suspected that Cas3 moved a couple of kilobases down the...

3. Common Injection Routes in Mice

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   ...injections are most often used when injecting a cell suspension to create xenograft models. Restraint: back scruffing...

4. Tips for a 1st time AAV user (by a Rookie AAV user)

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   ...solutions are carefully layered and then the viral suspension generated during AAV production is overlaid. ...

5. What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry

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   ...potentially impacting downstream experiments. The usual suspects, such as proteins, phenol, or guanidine salts,...

6. DNA Purification Without a Kit

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   ...Finally, DNA is precipitated with alcohol and resuspended in TE buffer. After this, the DNA is ready for...

7. New CRISPR Tools: Cas7-11 and PASTE

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   ... their initial findings.  The team originally suspected that this new Cas7-11 might be phylogenetically...

8. An Inside Look into Addgene's Dev Team

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   ...won’t tumble out of the sink and on to a poor unsuspecting pet.  The software equivalent to this would ...

9. Viral Production at Addgene

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   ... pellets containing viral particles are then resuspended in PBS, aliquoted, frozen, and stored at -80 ...

10. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

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    ... allows for targeted modification of known or suspected genes that regulate a desired phenotype. In fact...

11. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

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    ...experiments with too few replicates leading to suspect statistical significance (you can read more about...

12. A Control for All Seasons

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    ...interaction. When non-specific interactions are suspected, use a preadsorption control to confirm. In this...

13. Technique: Probe Phage Genomes for Host Binding Proteins

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    ... your membranes with PBST to remove salt, then suspend a freshly harvested dose of cells (preferably in...

14. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

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    ...you are reading this article then you probably suspect mycoplasma contamination in your cell culture or...

15. Important Considerations When Using AAVs

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    ...should still be taken when using them. There are suspected cases of insertional mutagenesis in humans (1)...

16. Typing CRISPR Systems

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    ...mechanism of type IV systems is not known, but it’s suspected that they don’t carry out the same types of adaptive...

17. Plasmids 101: Modular Cloning Applications and Kits

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    ...integration modules, and many more of the usual suspects. The Multiplex Yeast Toolkit builds on the YTK...

18. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

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    ...transfection, add csRNP and ssODN to the cell suspension, transfect and let cells recover for 2-5 days.Note...

19. CRISPR 101: Cytosine Transversion Editors

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    ...converts them back to Cs. In this case, the team suspected that the BER process was excising the incorrect...

20. Deep Dive: qPCR

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    ...slope generated should be less than 0.1.  If you suspect that amplification efficacy differences may be ...