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We narrowed to 84 results for: aav vectors expressing gfp

Showing: 41 - 60 of 84 results

  1. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    ...(tTA, also expressed by your transfer vector) binds to the TRE and activates the expression of your gene...distinct expression vectors to target cells - one containing the gene of interest (pTet-IRES-EGFP, pPRIME-Tet-GFP-FF3...your experiment using the lentiviral vector system. Viral vectors have been increasingly popular in fundamental...primary cells. Amongst the different vectors used, lentiviral vector constructs have proven very useful ...fluorescent sensors. Retroviral vector background Until the development of viral vectors, gene transfer into mammalian...un-transfectable cells, labs use viral vectors and particularly retroviral vectors because they target a broader...attractive choice for a gene transfer vector. Lentiviral vectors Although retroviruses are still in use...

  2. Hot Plasmids - February 2022

    Type
    Blog Post
    ...individually expressed proteins run in separate lanes. B: individually expressed and coexpressed proteins ...through two polycistronic expression vectors (3570 lanes/50 mLs) coexpressing a set of either 10, 30, 50...anti-FLAG-FB-GFP binding to FLAG-tag H2B for nuclear localization. (c) Applications of anti-FLAG-FB-GFP labeling...editing within a single expression cassette is advantageous to Cas9’s larger expression requirements (Figure... Figure 2: comparison of Cas 9 vs. Cas 12a expression cassettes when encoding multiple guides. While...opportunity for lab members or students new to protein expression and purification. Figure 3: The Penn State...Santilli, et al. 2021 Plasmids are used to express 10, 15, 20, 30, 40, 50, 60, 80 and 100 kD proteins...

  3. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ...sequence into distinct expression vectors using the enzyme BbsI. These expression vectors each contain Type...Each destination vector contains GFP, enabling you to select cells with high GFP expression. These cells ...Lentiviral Expression Systems (MuLE) Kit: This kit can be used to create lentiviral vectors expressing wt humanized...containing vector using Golden Gate assembly, and up to 8 gRNAs may be expressed simultaneously. Vectors for ...for ordered assembly into a destination vector that expresses Cas9. This is illustrated in the schematic...Cas9 or dCas9-containing destination vectors. Destination vector options include humanized wt Cas9, dCas9...enables users to express up to 7 gRNAs. The kit contains different destination vectors depending on the...

  4. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

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    Blog Post
    ...Brainbow adeno-associated viral vectors (AAV) available - AAV-EF1a-BbChT and AAV-EF1a-BbTagBY. These constructs...recombination, RFP is expressed. Cre can mediate one of two deletions to allow YFP or M-CFP expression; these deletions...inversion. The Brainbow-2.1 construct can express one of four colors (n-GFP, RFP, YFP or M-CFP.) The construct...associated with AAV; co-infection with both constructs produces a minimum of 8 colors. The use of AAV provides...editing may be the hot new way to manipulate gene expression, but other gene manipulation systems remain valuable...ways to spatially and temporally control gene expression, especially in in vivo models, and new Cre-lox...fluorescent protein in the construct, OFP (orange), is expressed when Cre is absent; stochastic Cre excision results...

  5. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...has developed a new AAV approach using regulatory elements that restrict expression to this cell type. ...for the research community. You can find the AAV vectors for infecting GABA-ergic interneurons here. ...low off-target activity; mammalian expression vector Rees H, et al. Nature Communications. 2017. PubMed...and ribozyme systems for multiplex gRNA expression. Expression of toolkit components can be controlled...gene editing. The bacterial and mammalian expression vectors for these CRISPR inhibitors are listed in...Species Bacterial Expression Construct SpCas9 Inhibition (E. coli) Mammalian Expression Construct SpCas9...tags for mTagBFP, TagRFPt, EGFP, mVenus, mCerulean3, mKOFP2) and empty vectors that have standard cloning...

  6. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...in an AAV transfer vector for in-vivo expression and plasmids optimized for dCas9-VPR expression in drosophila...protein expression constructs fused with epitopes or fluorophores, generate expression vectors with various...parasites that constitutively express Cas9 with a library of sgRNA expression vectors (containing ten guides ... 74307 pAAV-CMV-DIO-Magneto2.0-sNRPpA AAV 74306 pAAV-CMV-DIO-TRPV4-p2A-ferritin-sNRPpA AAV 74302 ... HEK293FT cells expressing a single gRNA targeting a single gene. The gRNA expressing cells were then ... to test multiple expression systems before they achieve the appropriate expression level for their gene...various promoters suitable for in vivo expression, and/or produce vectors to make mutant cell lines or transgenic...

  7. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ...of a GFP-Puro cassette into a given locus, just upstream of a stop codon. Briefly, the PITCh vector should...microhomologies in the PITCh vector ~20 bp 5’ and 3’ microhomologies are added to the GFP-Puro cassette via PCR...cloned into a vector containing Cas9 and the PITCh-gRNA. Step 3: Contransfect the PITCh vector with the vector...plasmids available from Addgene are perfect for expressing GFP from a given promoter, and the technique can... the GFP-Puro will be inserted just upstream of a stop codon. One potential concern is if the GFP-Puro...repair, making it easier to construct targeting vectors. Addgene depositor Takashi Yamamoto’s lab has harnessed...Target Chromosomes). Using their PITCh plasmids, GFP knock-in cell lines can be created in about a month...

  8. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

    Type
    Blog Post
    ...promoter is expressed. Cre-mediated deletion of one or several of these genes permits the expression of genes... from the default GFP (green) to RFP, CFP, or YFP fluorescence. In Brainbow 2.1, GFP is tagged with a ...fluorescence of the downstream OFP, GFP, or RFP. Cells that express the construct but do not undergo recombination...activate the expression of a different fluorescent protein. Therefore, each cell could express a unique combination...this mouse generates cells that randomly express either nuclear GFP, membrane CFP, cytoplasmic YFP, or cytoplasmic...copy of the transgene expressing either YFP or CFP, and cells that do not express Cre fluorescing with ...the low expression of XFP of Brainbow by incorporating a Tet-Off system to boost XFP expression. The increased...

  9. Hot Biosensors 2022: Year-End Roundup

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    Blog Post
    ...-mRuby3 (AAV1) pGP-AAV-syn-jGCaMP8f-WPRE (AAV Retrograde) pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE (AAV Retrograde...constructs: pAAV-CAG-dLight1.2 (AAV1) pGP-AAV-syn-FLEX-Ace2N-4AA-mNeon-ST A122D WPRE (AAV1) pAAV-hSynapsin1...pAAV-CAG-SomaGCaMP6f2 (AAV9) Plus, additional AAV serotypes of the most recent jGCaMP8 calcium sensors: pAAV-hSynapsin1...Retrograde) pGP-AAV-syn-FLEX-jGCaMP8m-WPRE (AAV5)   There are hundreds of other biosensors in Addgene’s catalog...are generated by inserting a circularly-permuted GFP into an intracellular loop of various G-protein-coupled...Figure 2: iGluSnFR3 displays enhanced brightness, expression, and sensitivity compared with earlier SF-iGluSnFR...GEVIs. Some constructs are also available as viral vectors (here and here).  Figure 3: JEDI-2P was identified...

  10. New Viral Vectors - Summer 2024

    Type
    Blog Post
    ...viral vectors repository since March.  Addgene is now offering an AAV2 CAG-driven tTA expresser from ...Addgene's Data Hub What is a Virus (and a Viral Vector)?  AAV Variables that Matter...doxycycline inducible (tet-off) gene expression! We've also added AAV1 and AAV5 preparations of several variants...viral service pAAV_hSyn1_nLightR AAV9 Biosensors Patriarchi New viral service pGP-AAV-syn-jGCaMP8m-WPRE...viral vectors in the Addgene repository  Name Serotype Category Depositor  Notes pAAV_hSyn-SIO-stChrimsonR-EGFP-P2A-PdCO-miniWPRE...What's new in Addgene's ready-to-use viral vectors repository? Quite a bit! In this post, we'll share...for more viral vectors? Browse the full viral services collection or check out Addgene's Data Hub to see...

  11. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...bacterial expression vector which gives close to 100% cloning efficiency and gene expression. The pUCXKT...custom-cloned vectors in a Multisite Gateway reaction with a desired destination vector expressing a separate...-TO) expression, or a promoterless construct. Pre-constructed Entry vectors containing Cas9, EGFP, mCherry...both the GFP and the RFP proteins in the nucleus. During the first round of translation, the GFP proteins...science.aaa3380 MuLE vectors for customized lentivirus production Finding a lentiviral vector backbone with ... SFFV) or inducible (CMV-TO) cDNA expression, sgRNA (U6) expression, shRNA/shRNA-miR30 constitutive (H1...enhancing target gene expression anywhere from 87x to 20,000x over endogenous expression (depending on the...

  12. Brain Initiative Collection

    Type
    Collection
    ...-NLS-GFP AAV expression of nuclear localized GFP with SV40 NLS Viviana Gradinaru 105448-AAV9 pAAV-hSyn-DIO-ChrimsonR-mRuby2...Fishell 83895-AAV1 pAAV-hDlx-Flex-GFP-Fishell_6 Cre recombinase-dependent GFP expression in forebrain ...Fishell 83895-AAV2 pAAV-hDlx-Flex-GFP-Fishell_6 Cre recombinase-dependent GFP expression in forebrain ...Fishell 83895-AAV8 pAAV-hDlx-Flex-GFP-Fishell_6 Cre recombinase-dependent GFP expression in forebrain ...Fishell 83895-AAV9 pAAV-hDlx-Flex-GFP-Fishell_6 Cre recombinase-dependent GFP expression in forebrain ...Fishell 83895-AAVrg pAAV-hDlx-Flex-GFP-Fishell_6 Cre recombinase-dependent GFP expression in forebrain ...element Gordon Fishell 83900-AAV1 pAAV-mDlx-GFP-Fishell-1 GFP expression in forebrain GABA-ergic interneurons...

  13. Hot Plasmids: Summer 2025

    Type
    Blog Post
    ...our blog post on AAV serotypes for more about the tissue tropism of these and other AAVs. Figure...them in our own AAV production processes. Check out our step-by-step protocols for AAV production, purification... In-Stock AAV inventory or use our Packaged on Request service for thousands of eligible AAV plasmids ....012.  New AAV6 and AAV11 packaging plasmids from Addgene By Meghan Rego Attention AAV users: in an ...plasmids to order viral vectors ready to use in your lab. Find Addgene’s AAV6 and AAV11 packaging plasmids here... mGold2. Top: TIRF-SIM imaging of COS-7 cells expressing LifeAct fused to mVenus, mGold2s, or mGold2t....but TnpB is small enough to be carried by viral vectors (~400 amino acids, less than a third of the size...

  14. Caltech Systemic Capsids

    Type
    Collection
    ...Broad tropism - AAV-PHP.eB, AAV-PHP.AX, AAV-PHP.eC Cell type-biased: Astrocytes - AAV-PHP.AX with GRE ...-B22 AAV ID Name Promoter Description Category PI 37825 pAAV-CAG-GFP CAG GFP Control Boyden AAV9-X1.1 ...Available MaCPNS1 AAV ID Name Promoter Description Category PI 37825 pAAV-CAG-GFP CAG GFP Control Boyden ...Available MaCPNS2 AAV ID Name Promoter Description Category PI 37825 pAAV-CAG-GFP CAG GFP Control Boyden ...Available CAP-B10 AAV ID Name Promoter Description Category PI 37825 pAAV-CAG-GFP CAG GFP Control Boyden ...Neurons - AAV.CAP-B10, AAV-PHP.N Broad - AAV-PHP.AX with GRE or transgenic animal L5 & Inhibitory - AAV-PHP.... Viral Vector Packaging Service AAV Caltech Systemic Capsids Viral Vector Packaging Service...

  15. Retrograde AAV viral preps

    Type
    Collection
    ...Viral Vector Packaging Service AAV Retrograde AAV Viral Vector Packaging Service: Retrograde AAV As part...Category PI 37825 AAV-CAG-GFP CAG GFP Control Edward Boyden 51502 AAV pCAG-FLEX-EGFP-WPRE CAG EGFP, Cre-dependent...Gradinaru 83900 pAAV-mDlx-GFP-Fishell-1 Dlx GFP Control Gordon Fishell 83895 pAAV-hDlx-Flex-GFP-Fishell_6 Dlx...Nectow 50363 AAV phSyn1(S)-DreO-bGHpA Syn Dre expression Recombinases Hongkui Zeng 51669 AAV phSyn1(S)-FlpO-bGHpA...58880 pAAV-Syn-ChR2(H134R)-GFP Syn Activator Optogenetics Edward Boyden 59170 pAAV-Syn-Chronos-GFP Syn ...mCherry)-EGFP EF1a Color-flipping switch. Expresses NLS-mCherry in the absence of Cre, and expresses NLS-EGFP...Cre expression Recombinases James M. Wilson 105551 pENN.AAV.CamKII.HI.GFP-Cre.WPRE.SV40 CamKII GFP-tagged...

  16. Brain Armamentarium

    Type
    Collection
    ...Daigle James M. Wilson 37825-CAP-B10 pAAV-CAG-GFP AAV expression of GFP from the CAG promoter Edward Boyden...Boyden Viviana Gradinaru 37825-CAP-B22 pAAV-CAG-GFP AAV expression of GFP from the CAG promoter Edward Boyden... Gradinaru 37825-CAP-MaCPNS1 pAAV-CAG-GFP AAV expression of GFP from the CAG promoter Edward Boyden Viviana... Gradinaru 37825-CAP-MaCPNS2 pAAV-CAG-GFP AAV expression of GFP from the CAG promoter Edward Boyden Viviana...-PHPeB CN1851-rAAV-hI56i-minBglobin-iCre-4X2C-WPRE3-BGHpA AAV vector for Cre expression in forebrain GABAergic... Tasic Melina Fan 221463-AAV1 pAAV_BiPVe4_eGFP AAV construct expressing eGFP driven by chandelier cell-targeting... Viviana Gradinaru 213947-AAV1 pAAV_BiSSTe4_GCaMP6f AAV construct expressing GCaMP6f driven by SST interneuron-targeting...

  17. AAV Molecular Tools

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    Collection
    ... Viral Vector Packaging Service AAV Molecular Tools Viral Vector Packaging Service: Molecular... Long Overexpression Tools These AAV encode various genes for overexpression. ID Name Expression System...)-inducible expression systems . ID Name Expression System Activity Serotype PI 99118 pAAV-CAG-tTA CAG-driven... ID Name Expression System Activity Serotype PI 51509 AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE Syn-driven...Serotype PI 124364 pAAV-FLEX-DTR-GFP CBA-driven, Cre-dependent Cre-dependent expression of diphtheria toxin...-inducible expression of EYFP 1 Viviana Gradinaru Tools for Affinity Purification These AAV encode tools...Ablation These AAV encode tools that can be used for cell ablation. ID Name Expression System Activity...

  18. Viral Production

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    Collection
    ... later, Cre-dependent GFP expression was detected with direct fluorescence. GFP was not detected in the...Electron micrograph of AAV vector preparation shows that the vast majority of the vectors consist of full particles...using our virus protocols . Browse our AAV vectors collection. AAV Data Hub Lentivirus Production Lentiviral... and quality control are described below. AAV Production AAV distributed by Addgene has been produced ... with viral vector manufacturing facilities, such as the University of Pennsylvania Vector Core. Transfections...Pennsylvania Vector Core. In general, titering is performed by the facility that produced the viral vector lot....determine which facility produced your viral vector lot. At Addgene, AAV particles are titered by droplet digital...

  19. Zhang Lab CRISPR Page

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    Collection
    ...transfection : 61422 : Expresses dCAS9-VP64 activator with 2A GFP 61423 : Expresses the MS2-P65-HSF1 activator...2015). The dual vector system uses S. pyogenes Cas9 (SpCas9), using one vector to express SpCas9, and another...sgRNA to a constitutively Cas9-expressing mouse. Described here are AAV vectors that can be combined with ... lung adenocarcinoma. Delivery of a single AAV vector ( AAV-KPL ) in the lung generated loss-of-function...#60958) contains pAAV-U6::sgRNA(SapI)_hSyn-GFP-KASH-bGH (SpGuide acceptor). This is a AAV plasmid for sgRNA...to sgRNA expression. This plasmid contains two expression cassettes, Cre recombinase-2A-EGFP-KASH and ...

  20. Control AAV Preps

    Type
    Collection
    ... Viral Vector Packaging Service AAV Controls Viral Vector Packaging Service: Control AAV Browse our...our control AAV that express fluorescent proteins under general promoters. These AAV can be used to compare...Fluorophore/Tag Activity Serotype PI 37825 AAV-CAG-GFP CAG GFP Constitutive 1, 2, 5, 6, 8, 9, 11, rg*, PHPeB...different serotypes. Serotype AAV1 AAV2 AAV5 AAV6 AAV8 AAV9 AAVrh10 AAV11 Retrograde AAV PHP.eB PHP.S PHP.V1 CAP-B10...CAG-NLS-GFP CAG NLS-GFP Constitutive PHPeB Viviana Gradinaru 105530 pAAV.CMV.PI.EGFP.WPRE.bGH CMV EGFP Constitutive...83895 pAAV-hDlx-Flex-GFP-Fishell_6 Dlx GFP Cre dependent 1, 2, 8, 9, rg* Gordon Fishell 83894 pAAV-hDlx-Flex-dTomato-Fishell... PHP.eB, PHP.S Edward Boyden 83900 pAAV-mDlx-GFP-Fishell-1 Dlx GFP Constitutive 1, 2, 5, 8, 9, rg*, PHP.eB...
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