CRISPR-mediated Plant Base Editors
Type
Blog Post
... to a mutant TadA (TadA*) that satisfactorily accepts DNA as its substrate (see figure 2).
The...NNNRRT
8
ABE
SpCas9
TadA-TadA*
NGG
9-12
SpCas9-VQR
TadA-TadA*
NGA
8
SaCas9
TadA-TadA*
NNGRRT
9
SaCas9...available for plant systems. Target-AID based (CBE-2)- and rAPOBEC based (CBE-1)- CBEs are successful...based CBEs are known to deaminate cytosine placed in 2-6 bp and 4-8 bp, respectively, of the protospacer....PMID: 27096365. PubMed Central PMCID: PMC4873371.
2. Nishida, Keiji, et al. "Targeted nucleotide editing...SaCas9-KKH
TadA-TadA*
NNNRRT
8
SpCas9-VRER
TadA-TadA*
NGCG
8
Tips for designing base editing experiments... of different basic BE technologies and their adaptation for plant genome editing.
Cytosine base editors...