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We narrowed to 69 results for: addgene/1000

Showing: 41 - 60 of 69 results

  1. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    ...CRISPR Libraries Resources on Addgene.org Browse Pooled Libraries at Addgene ...In addition to single plasmids, Addgene also distributes pooled plasmid libraries containing hundreds...million plasmids. These libraries are some of Addgene’s most exciting and versatile offerings! We recently...plasmids are amplified and none are lost. Each Addgene library has its own depositor transformation and...components of the library are represented. Typically, Addgene uses electrocompetent Stbl4 cells when performing...transformation efficiency. For a typical library, Addgene usually does four to eight electroporation reactions...a gRNA library, this is less of a concern, but Addgene still recommends plating the transformation mixture...

  2. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type
    Blog Post
    ...are many more useful viral vectors available at Addgene: Follow our viral vector blog posts for updates... Get the ready-to-use lentiviral prep from the Addgene viral service. CRISPR technology lentiCRISPR v2...PMCID: PMC4830361. Additional Resources on the Addgene blog Viral vector topic page Beginners guide to...Plasmid Kit Retroviral Barcoding Library (Kit # 1000000097) The Winslow lab barcoding library consists of...Multiple Lentiviral Expression System Kit (Kit #1000000060) The MuLE (Multiple Lentiviral Expression) system...guide to all things lentivirus Resources on Addgene.org Gamma-retrovirus Guide Lentivirus Guide ...

  3. Antibody Guide

    Type
    Guide
    ...Science Guides Antibody Guide Antibody Guide View Addgene's Antibody Collection Jump To: Antibody Guide Production...applications, visit the Antibody section of the Addgene Protocols page. Antibody Applications - Quantification...sonication to break DNA up into fragments of 300-1000 bps in length. Note: This sonication process may...intensive. Ready to put your new knowledge to use? Addgene now makes ready-to-use recombinant antibodies made...

  4. Bacterial Expression Systems

    Type
    Collection
    .... Additional Addgene Genome Engineering Resources Learn more about CRISPR with Addgene's CRISPR Guide ... Consortium Modular Assembly Kits (Addgene #1000000005, 1000000010) contain plasmids and an engineered...localization to the periplasm. The pCri System (Addgene #1000000058) plasmids can be utilized for heterologous...Pichia pastoris . The EcoFlex MoClo Toolkit (Addgene #1000000080) features a library of promoters and purification... The cpFRET - FRET-based Biosensors Kit (Addgene #1000000021) is a vector library for the generation of...ratiometric measurements. The MoBiFc Toolkit (Addgene #1000000222) is a collection of Golden Gate compatible...activation or repression. The BIOFAB plasmid set (Addgene #1000000037) contains specific bacterial promoters that...

  5. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    ...10.1128/mbio.02364-19 More CRISPR Blog Posts from Addgene: CRISPR 101: Homology Directed Repair CRISPR...Potential. PLoS Genet 5:e1000683 . https://doi.org/10.1371/journal.pgen.1000683 Bétermier M, Bertrand P...

  6. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ... distribution via Addgene. For a full list of Addgene's viral vectors See Addgene's Current AAV inventory...for distribution at Addgene: Penn ID Addgene ID Name Function PI AV-1-49531P 100040-AAV1 pAAV.hSyn1.Twitch2B.WPRE.SV40...Penn Vector Core and Addgene partnership to provide AAV viral preps, includes listing of transfer status...Pennsylvania Vector Core (Penn Vector Core) and Addgene previously partnered to provide high-quality AAV...be helpful to the scientific community. Penn ID Addgene ID Name PI AV-1-18916P 18916-AAV1 AAV-FLEX-rev-...36857M 100038-AAV1 pENN.AAV.hSynap.Flex.ArcLightDco.WPRE.SV40 Vincent Pieribone AV-1-36857P 100037-AAV1 ...Looger AV-1-ALL856 100048-AAV1 pAAV.CAG.LSL.tdTomato Hongkui Zeng AV-9-ALL856 100048-AAV9 pAAV.CAG.LSL....

  7. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    ...crime, Cascade. Find Cascade-Cas3 plasmids in Addgene's collection! A “complex” system Cas3 is part of...and Genome Editing, 3–4, 100013. https://doi.org/10.1016/j.ggedit.2022.100013 O’Brien, R. E., Bravo, J... CRISPR-Transposons Additional resources on Addgene.org CRISPR guide Cascade-Cas3 Plasmid collection...

  8. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    ....3390/ijms22105084 Additional resources on the Addgene blog Learn about the INTEGRATE system for bacterial... out of the laboratory. F1000Res 9:135. https://doi.org/10.12688/f1000research.21018.1 Munoz-Lopez M, ...

  9. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...pick a library. The pooled CRISPR libraries in Addgene’s collection differ based on some important criteria...filtered list of the CRISPR pooled libraries in Addgene's collection. Library Type Activation Barcode Knockout...Blast 1000000173 Knockout Mouse Liu 3rd 10 185,836 Mouse GeCKO v2 1000000052 (1 plasmid) 1000000053 (2 plasmid...Varies Bassik Mouse CRISPR Knockout Library 1000000121 — 1000000130 Knockout Mouse Bassik 3rd 10 Varies Berger...83978 1000000091 Activation Human Weissman 3rd 5 10 104,540 209,080 CRISPRa-v2 83996 1000000093 Activation...83969 1000000090 Inhibition Human Weissman 3rd 5 10 104,535 209,070 CRISPRi-v2 83987 1000000092 Inhibition...Birsoy 3rd 8 20,051 Human GeCKO v2 1000000048 (1 plasmid) 1000000049 (2 plasmid) Knockout Human Zhang 3rd...

  10. Typing CRISPR Systems

    Type
    Blog Post
    ...and Genome Editing, 3–4, 100013. https://doi.org/10.1016/j.ggedit.2022.100013 Additional resources on ...your CRISPR vocabulary Additional resources on Addgene.org CRISPR guide CRISPR plasmids CRISPR references...

  11. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...good option for your experiment? Look it up in Addgene’s Data Hub and the CLOVER repository of published...Table 1: Systemic capsid plasmids available from Addgene Capsid Published Tropism Model Organism...most commonly requested systemic capsid in the Addgene repository. Like its parent AAV-PHP-B, it can be...repository of published systemic AAV experiments and Addgene’s AAV Data Hub to learn more about the capsid you...significant contributions from Cynthia Arokiaraj. Addgene would also like to thank Viviana Gradinaru and ...10.1038/s41596-018-0097-3 More resources on the Addgene blog Viral Vectors 101: AAV Variables That Matter...Research in Neurobiology, 4, 100086. https://doi.org/10.1016/j.crneur.2023.100086. Challis, R. C., Ravindra...

  12. Zinc Finger Consortium: OPEN Reagents

    Type
    Collection
    ...publication was a gift from Keith Joung (Addgene kit # 1000000013)" For your Reference section: Oligomerized...article in which they were created, and include Addgene in the Materials and Methods of your future publications... Zinc Finger Consortium: OPEN Reagents (Kit # 1000000013 ) Depositing Labs: Keith Joung This kit consists...

  13. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

    Type
    Blog Post
    ... in a previous Addgene blogpost and more recently in a paper published in F1000Research on November 2016...Science Careers Resources on Addgene.org Find Career Opportunities at Addgene  ...Research @FORsymp.   Additional Resources on the Addgene Blog Read Gary's Previous Article: Changing Labor...

  14. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    ...biomedical_research_wgreport.pdf Resources at Addgene Learn More about Careers in Science in Our Career...Advice on Networking at Scientific Meetings from Addgene Executive Director Joanne Kamens Learn how to be...Research: a perspective from junior scientists." F1000Research 3 (2014). Pubmed PMID: 25653845. Pubmed Central...

  15. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    ... attL-containing entry vector.   Pro Tip: Addgene also has ready-made entry clones available for ...expression system in just one recombination step. Addgene’s ready-made entry clones can be used with a large...of the Gateway Multisite plasmids available at Addgene, including the Frew Lab Multiple Lentiviral Expression...deposited their own Gateway-compatible plasmids with Addgene. These can be used to express genes in a variety..., MA: Cell Press). Additional Resources on the Addgene Blog Try Out FastCloning Learn About Methylation...Drawbacks. Clon Transgen 4:138. doi:10.4172/2168-9849.1000138 2. Hartley JL. Use of the Gateway System for... to Quickly Verify Your Clones Resources on Addgene.org Find Plasmid Cloning Protocols Watch Our Diagnostic...

  16. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    ...and updated in 2022 by Lucie Wilson. Lucie is an Addgene co-op from Northeastern University.  There can ...& Parkinsonism. https://doi.org/10.4172/2161-0460.1000439 6. Wu et al. 2020. . “Advances in CRISPR/Cas-based...

  17. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    ...more. Being that this is the Addgene Blog, I’ll, of course, mention Addgene as a source of sequenced and...event, I gave my own talk on reproducibility at Addgene and here I summarize what I learned both in preparation...31 - 49:45 - Reagent Sharing - Susanna Bachle, Addgene the nonprofit plasmid repository 49:45 0 1:18:45...viral vectors. When working with a plasmid from Addgene, you can be confident that you’re using the actual...hosted by individuals and by organizations like Addgene (a good example is our series of blog posts on ...Reproducibility at Protocols.io Additional Resources on the Addgene Blog FPbase: A new community-editable fluorescent.... Finally there are new publishing outlets like F1000, PeerJ, PLoS, and bioRxiv that make it easier to...

  18. Optogenetics AAV Preps

    Type
    Collection
    ...preparations of optogenetics plasmids available in Addgene's collection. Opsin Excitatory Wild-type ChR2 ChR2...GFP Syn ChR2/H134R GFP Constitutive 8, rg* Boyden 100054 pAAV.CAG.hChR2(H134R)-mCherry.WPRE.SV40 CAG ChR2...] CAG ChrimsonR tdTomato Flp dependent 8 Boyden 100049 pAAV.hSynap.ChETA(E123T/H134R)-eYFP.WPRE.hGH Syn...Syn ChETA EYFP Constitutive 1, 9 Deisseroth 100050 pAAV.CaMKIIa.ChETA(E123T/H134R)-eYFP.WPRE.hGH CaMKII... EYFP Constitutive 1, 5, 9, PHPeB Dimidschstein 100061 pAAV-Ef1a-DIO C1V1 (t/t)-TS-mCherry EF1a C1V1 (...

  19. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

    Type
    Blog Post
    ...(Che and Khavari, 2017). Find APEX plasmids at Addgene! Since the development of BioID and APEX, optimized...These newer tools are also currently available at Addgene.  BioID2: A smaller improved promiscuous biotin...PMCID: PMC5467174. Additional resources on the Addgene blog Plasmid 101 Protein Expression Plasmid 101...proximity-based labeling methods for interactome mapping. F1000Research, 8.  ...

  20. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    ...resources on Addgene.org  Addgene's Plasmids 101 eBookAddgene's Molecular Biology ReferenceAddgene's Restriction... performing restriction digests on plasmids at Addgene, we sometimes observe something odd in our uncut... the short-read Illumina MiSeq sequencing that Addgene uses.) However, when some researchers received ...described in this article.   Long-read sequencing Addgene uses Illumina MiSeq NGS to perform whole plasmid...Restriction Digest Protocol More resources on the Addgene blog:  Plasmids 101: What is a plasmid? Plasmids ... in the comments below or contact us at help@addgene.org. Resources and References References Bacolla...Electrophoresis. 2010 Jul;31(14):2436-41. doi: 10.1002/elps.201000061. PMID: 20589859. Levene SD. Analysis of DNA topoisomers...
Showing: 41 - 60 of 69 results