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We narrowed to 209 results for: dap

Showing: 41 - 60 of 209 results

  1. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type
    Blog Post
    ...immune system. The techniques used here could be adapted to studying other systems in which there is large... revealing the cellular behaviors that mediate adaptive and innate immunity in diverse tissue environments... to better understand T cell initiation in the adaptive immune response. It was known that T cells were...

  2. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    ...Choose from different “Options”, including adding adapter sequences for cloning purposes.  Press run and... well as the suggested gRNA sequence including adapters. Output_UserInputParams: Contains all the information...subsequently cloned into your vector of choice using the adapter sequences that you have specified.   Run your...

  3. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    ... absorbed over the photons emitted. For example, DAPI is much dimmer than Alexa Fluor 488, and thus it...without causing undue toxicity. A prominent example is DAPI, a dye that binds specifically to the AT-rich regions...Lavis Lab (Grimm et al., 2017). These dyes were adapted into ligands for HaloTag® and are currently offered...

  4. Deep Dive: qPCR

    Type
    Blog Post
    ...Table 1: Features of various qPCR chemistries. Adapted from Wong, 2018   Primers Design of primers...method is the Standard Curve method. See Table 2, (adapted from Wong, 2018) for a comparison of different ...comparison of different qPCR analysis methods. Table adapted from Wong, 2018.    🔥Hot Tip🔥 Negative...

  5. What's New in CRISPR - Winter 2018

    Type
    Blog Post
    ...Cas14 targets and cleaves ssDNA which they have adapted for detecting SNPs. Learn more from this Addgene...discovered in 2017. Now Dominik Niopek’s lab has adapted anti-CRISPR proteins for optogenetic control of...

  7. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    ...antibody-dependent cellular phagocytosis (ADCP). Figure adapted from van Erp, et al., 2019.    Antibody-dependent...Song, WC. Complement and its role in innate and adaptive immune responses. Cell Res 20, 34–50 (2010). https...

  8. Embracing Serendipity: A Crucial Element in the PhD Journey

    Type
    Blog Post
    ..., the ability to embrace serendipity fosters adaptability, a crucial skill for a successful research career...research but also cultivate a mindset that is adaptable, creative, and poised for groundbreaking discoveries...

  10. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    ...researcher. There existed, therefore, a need for an adaptable tool that could continuously monitor multiple ...- Overview of the neural and gut fiber system (adapted from Sahasrabudhe et al., 2023).   To develop...

  11. What the HEK?

    Type
    Blog Post
    ...adherent monolayer, though derivatives have been adapted for growth in suspension. Of the many derivatives... allows for large scalability, and can also be adapted for serum-free growth, which significantly reduces...

  12. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    ...recombinase-based state machines and how they function. Adapting Toehold Switches to Detect Zika Virus The RNA-... understanding of Cas9 biophysics! Casilio: An Adaptive, Multitasking “CRISPR-OS” In this post, Albert...

  13. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    ...career paths and things you can do to prepare. Adapt to WFH while parenting Keep your kids on a schedule... your work schedule as possible so that you can adapt to your kids' needs. Plan ahead. Think about and...

  17. Hot Plasmids - January 2023

    Type
    Blog Post
    ...nuclear (MALAT1) and cytoplasmic (CKB1) RNA target. Adapted from Figure 3 Colognori et al 2022.   Find CRISPR...temperatures, and in the presence of oxidizing agents. Adapted from Fig. 2 Campbell et al, Nature Methods ...

  18. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    ...their devices, which makes it very difficult to adapt the device to one's own samples and limits the use...to as 'frugal science', existing components are adapted and combined with creativity and feedback from ...

  19. What's New in CRISPR - November 2021

    Type
    Blog Post
    ...packaging into virus-like particles. Feng Zhang’s lab adapted the native PEG10 RNA sequences to instead drive...delivering gene editing tools into cells. Image adapted from Segel et al. 2021.   Prime editing improvements...

  20. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    ...features and evolved PAM sites, making Cas9 an adaptable choice for many genome engineering endeavors. ...   Choosing a Cas While Cas9 may be the most adaptable Cas currently available, the other Cas proteins...
Showing: 41 - 60 of 209 results