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We narrowed to 335 results for: dre

Showing: 41 - 60 of 335 results

  1. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

    Type
    Blog Post
    ...Abhi Aggarwal.   Brandán Pedre Pérez: Redox biosensors Brandán Pedre Pérez is a postdoc at the German...Congratulations to the five winners! Abhi Aggarwal Brandán Pedre Pérez Elizabeth Hubbard Haoyi Qiu He Tian Every... have used it to detect glutamate release from hundreds of synapses at once. “This work has only been ...during oxidative stress. To monitor this activity, Pedre Pérez’s biosensor fuses MPST to roGFP2. Because ...the processes that happen inside the cell,” says Pedre Pérez. Figure 2: Micrographs of the biosensor...

  2. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    ...new. Chapter 2, “The Professional Scientist,” addresses the challenges of succeeding in science if you...value here is seeing them all in one place. Can we address problems in scientific publishing without acknowledging...Chapter 11)? Even if some of the problems are addressed in a somewhat cursory manner, their inclusion ...genuinely shocking. Mainstream media outlets have addressed CRISPR frequently over the last several years,...over 1,000 authors. The chapter on deep learning addresses the dilemma of creating a technology that could... sections on optogenetics and CRISPR of course address some of the ethical implications of using these...

  3. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    ...FDA-approved drugs. See our inventory of DREADDs / Chemogenetics AAVs New DREADDs Plasmid Serotype Name 75033...Name 119036  AAV1  pAAV-hsyn-flex-Voltron-ST DREADDs / Chemogenetics AAV We have also expanded our inventory...activating and inhibiting neurons. Minimally-tagged DREADD AAV from the Richie lab are especially useful for...primates, where fusion fluorescent proteins can affect DREADD trafficking and localization. The Sternson lab ...

  4. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    ...83896 AAVrg pAAV-hDlx-GiDREADD-dTomato-Fishell-5 83897 AAVrg pAAV-hDlx-GqDREADD-dTomato-Fishell-4 ...SARS-CoV-2 proteins ChR2 for targeted expression DREADDs GFP dependent Cre and FLPo And more!  New lentiviral...plasmid encoding a Cre dependent version of the DREADD hM3D is now also offered as AAV1 (along with AAV...AAV2, AAV5, AAV8, and AAV9). Once activated, this DREADD acts through the Gq pathway and promotes neuronal...

  5. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    ...cloning techniques and troubleshooting methodologies drew me to learn more about the process that was used...driven by two CMV promoters (orange). Figure from Andrews, et. al., Elife  A limitation of hybridoma ...recombinant antibodies in adult rat brain. Figure from Andrews, et. al, Elife. Detailed figure legend here.   ...recombinant antibody plasmid collection References Andrews NP, Boeckman JX, Manning CF, Nguyen JT, Bechtold... Andrew Hempstead...

  6. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    ...consists almost entirely of viruses expressing DREADDs from Bryan Roth’s lab at the University of North...floxed human muscarinic receptor 4 (Gαi-coupled) DREADD fused to mCherry, all under the control of the ... cre-dependent, meaning that once injected, the DREADD will only be expressed in cells that express cre...further specifies neuron-specific expression of the DREADD (Kügler et al., 2003), which can then be exclusively...mice, rats, or non-human primates. To activate the DREADD, CNO is delivered either through drinking water...

  8. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    ...innovation will address a variety of challenges and impact the field - its encouraged to dream big! It is...technologies should offer in order to build upon and address some, if not all, of the limitations of current...sets the stage for why your funded proposal will address these limitations and lead to new milestones in... the project and ii) how you, the expert, will address the research challenge. Now is the time to make...

  9. How to Prepare for an Industry Interview

    Type
    Blog Post
    ...yours counts. Dress professionally but still like yourself. It’s better to be overdressed rather than underdressed... underdressed, but read emails from HR carefully because sometimes companies have relaxed dress codes ...interviews. You can also ask HR for clarification on the dress code for the interview. Greet each interviewer and...

  10. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...signaling. For more information on DREADDs, please see the Roth lab's DREADD users blog. New CRISPR plasmids...alleles off or on enables this method to rapidly address questions of tissue-specificity and cell autonomy...bacteria are time-consuming and involve many steps. The Drew Endy and Keith Shearwin labs have developed a new...al., ACS Synth Biol 2013 Sep 20;2(9):537-41. DREADD-based chemogenetic technologies After several...Receptors Exclusively Activated by Designer Drugs (DREADD) plasmids on his own, UNC-Chapel Hill's Bryan Roth...each recombines independently, generating tens or hundreds of possible combinations (depending on the number...

  11. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type
    Blog Post
    ...but proceed with caution! Loading controls only address one type of error that can impact your western ...red) influence these relationships and must be addressed with separate controls (green). Created with BioRender.com... for different loading controls So how can you address these errors without making incorrect assumptions...blotting quantification” (Gilda & Gomes, 2013). To address this source of error, it’s important to determine...Resources and References References Aldridge, G. M., Podrebarac, D. M., Greenough, W. T., & Weiler, I. J. (2008...

  12. How Do I Place an Addgene Order Using a PO Number?

    Type
    Blog Post
    ...regarding the order will be sent to the contact email addresses provided. How do I place an order using a PO number...organization in this field. Please also enter the billing address for your organization. We will contact you directly...facility, we will send an invoice to the billing address that you listed during checkout. This invoice will...additional questions about our ordering process not addressed in this article, check out our Help Center article...

  13. Advice for Choosing a Research Project

    Type
    Blog Post
    ...impact be in a best case scenario? If you get your dream results, what will your paper look like? What will...complexity of biology and break apart a piece that can address a topic in a mechanistic way using the technology...passions and dive into a project that aligns with your dreams. He also highlights the importance of combining...Choosing a Research Project Finding and Joining Your Dream Lab Advice for Choosing a Research Project Advice...

  14. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    ...community for addressing these important questions that the scientific method cannot address. Listen to ...changing". Dr. Yamanaka himself, during his keynote address, appeared almost incredulous that the field has...Mummery, Lorenz Studer, and Deepak Srivastava--addressed a battery of questions regarding topics such as...

  15. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    ...student from applying to the opportunities of their dream.  To help students focus on the application process...limit all distraction. On the day of the interview, dress the part, and be consistent with your personal statement...you a hand to get you into that program of your dream.  “Chance favors the prepared mind” by Louis Pasteur...educate parents about resources that can help their children thrive academically. Rose is the co-founder of...

  16. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    ...which are designed to support new approaches that address the need to train our young scientists for futures...Graduate education programs are also beginning to address these issues independently; for example, Harvard... Paula Stephan, professor of economics at the Andrew Young School of Policy Studies at Georgia State...Professor Stephan will be delivering the keynote address at the symposium. Eve Marder, professor of Biology...

  17. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

    Type
    Blog Post
    ...of the antibody chains (Crosnier et al., 2010; Andrews et al., 2019). Finally, scientists can perform ...plasmid sharing, the CRISPR community has created hundreds of useful variants of Cas9. By providing access...imagined yet. References and Resources References Andrews NP, Boeckman JX, Manning CF, Nguyen JT, Bechtold... IC, Tandon AK, Anderson S, Bladen CL, Jones B, Aldred SF, Bestagno M, Burrone O, Maynard J, Ferrara F...

  18. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    ...The cover letter is a great place to proactively address questions about your application. These could be... good candidate for the position. You can also address any missing experience or skills, often by pointing...your experience matches the job requirements, and address any unusual circumstances or proactively answer... than a page. Conciseness is key! If possible, address to the professor or hiring manager. Otherwise, ...

  19. Important Considerations When Using AAVs

    Type
    Blog Post
    ...Receptors Exclusively Activated By Designer Drugs, DREADDs) and optogenetics as tools to investigate the roles... Much of the previous literature using AAVs for DREADD delivery indicates ~7-21 days are required for ...anatomical mapping of circuits In our lab, we use AAV-DREADD complexes to study neural function. Often the reporter... Manitoba. Her project is examining the use of DREADDs (Designer Receptors Exclusively Activated By Designer...

  20. 6 Steps to Submitting a Resume That Gets Seen

    Type
    Blog Post
    ...interesting to you. Many job postings will result in hundreds of applicants the day they are posted.  If your...letters and even resumes The cover letter must address how you are qualified and why you are interested...up especially in large companies. It is fine to address the cover letter “To Whom it May Concern” as you...
Showing: 41 - 60 of 335 results