We narrowed to 315 results for: idua

...screening success? Before you start analyzing individual clones, it’s wise to quickly assess whether a...haystack before going on to generate and screen individual clones. If a clonal line isn’t your end goal,... can determine if it is worthwhile to isolate individual clones from that population. You can additionally... a bulk population and also identify edits in individual clones. The drawback is that you must generate...

...did, however, find it very hard to speak with individuals who had very strong beliefs in traditional medicine... can also get a lot of office politics (many individuals trying to divert more resources towards their...their projects and away from other individuals projects, this can cause conflicts and favoritism, as well...than was anticipated and there can be a glut of individuals with similar skill sets. This is not to say that...

...instance there are few resources to help get individuals started on the path to internationalization and...development and training in a globalized world where individuals can exchange ideas, network, collaborate, and... supportive and protective environment where individuals are empowered to productively navigate through...Country. Mentees represented a diverse group of individuals with technical and career path needs, and came...

... death. The Wu lab library consists of 77,406 individual gRNAs targeting a total of 20,121 genes within...stocks in a single plate with every plasmid or as individual plasmids shipped as agar stabs. Iverson, et ...these methods can be used to easily modify an individual locus within the genome, they tend to show their... as prior optimizations are required for each individual target species. Indeed, in order to use MAGE,...translational protein fusion would be advantageous when individual protein expression is preferred or required, ...guide RNA transcript and a repair template for an individual tagging event. Commonly used sequences, including...

...variety of antibodies to be produced in a single individual, giving a high probability that an antibody will...as immune responses have high inter- and intra-individual variability. Other Affinity Reagents There are...sonication process may need to be optimized for individual assays. Clear cell debris by sedimentation. Proceed...use a nuclear stain, such as DAPI, to identify individual cells. These stains also emit fluorescent emissions...excitation, and assay readout vary widely depending on individual assay conditions. Controls for visualization ...experiments often use a nuclei marker to identify individual cells and additional markers to distinguish cell...

... ascribed relative to the basic needs of each individual; the  community as a whole has yet to settle ...Instead, I have broken down the tools into the individual features that make each tool unique. The CRISPR...that it is all but impossible to fairly judge individual tools. I believe at this time it is only possible...possible to objectively break the tools into their individual offerings and allow you to select them based ...

... course of the class, the moderator discussed individual sections of a paper, the abstract, figures, introduction...class, we met as a group for sessions covering individual portions of the manuscript. Stephen Blanke, Beronda...generating a quilt with your lab mates, you add the individual figures and results to make the final product...

...SDS-Page gels. A: individually expressed proteins run in separate lanes. B: individually expressed and coexpressed...60, 80 and 100 kD proteins in E. coli, either individually (~20,000 lanes/50 mLs) or through two polycistronic...

...Letters of recommendation wisdom  Reach out to 2-3 individuals in your professional circle to vouch for the ...for internship applications. Thus these noble individuals are inundated with requests. The easier you make...the wrong approach. It is very likely you know individuals in your circle that will also benefit from these...

...exciting new tool that allows researchers to individually label millions of mammalian cells through lentiviral...ClonTracer library allows a researcher to label individual members of a starting population of cells with...multiplicity of infection (MOI) such that each individual cell receives only one barcode. The abundance...

...make their own decisions about genetics, both as individual consumers of technologies and as societal participants...three years. pgEd is a small team comprised of individuals from diverse backgrounds and disciplines, and...is to liaise with different organizations and individuals, to identify ways of collaborating, and to organize...

...antibiotic-resistant colonies, use a Sharpie to circle 12-48 individual colonies by (carefully) holding plates up to ...identified from the plate circling- are then individually punched out, gently sucked up into the pipettor...useful to scientists who wish to rapidly isolate individual cell clones with different expression characteristics...

...lines, looking to uncover genetic dependencies of individual tumors, which can lead to new cancer targets ...analysis. Their work revealed that cell lines from individuals of African descent have approximately 20% more...particularly in cancers that are more common in individuals of African descent.Correcting the bias  While...

...of the experiment would require access to each individual video. To PLOS’s credit, the organization subsequently...recording are highly variable and usually left to individual researchers. Yet accurate and comprehensible ...appears to leave the scope of data to be shared to individual authors. Minimally, raw but sufficiently annotated...

...After that, the next gates fully depend on your individual experiment. Practical example: lymphocytes in...strategy, I can, of course, also draw multiple individual gates around the populations of interest (Figure...of a quadrant gate, I am free to use multiple individual gates. Note that in this case, square gates were...

...immunodeficiency syndrome (AIDS) in untreated individuals. Symptoms of AIDS include rapid weight loss and...opportunistic infections not usually seen in healthy individuals. Antiretroviral therapy (ART) can virtually eliminate...CRISPR. CD4+ T-cells were isolated from healthy individuals, expanded, and infected with HIV-1. For two HIV...

...red-green). Even better, show each channel in individual panels, then the multicolor overlay; this helps...primary antibody in your experiment). Confirm each individual target is stained correctly Image a set of samples...label only one target in each. Compare these individually-labeled samples with the results of your multiplex-labeled...

...consistently welcoming. This bears repeating. Individuals that work in science policy are a treasure--they...do to creatively connect seemingly disparate individuals and ideas toward solving one problem. It often...school or a postdoc. But some are also open to individuals from mid- to late-career backgrounds. In fact...

... the initial stable cell pool (pool) and from individual cell expansions (monoclonal, 1-5). Wild-type ...maintenance involved. Also it was really fun to find individual colonies growing out in the 96-well dish. My ...control GFP gRNA (BRDN0000561167) (80034-LV) were individually used to transduced stable pools of Cas9-A549...

... at multiple levels to generate anything from individual transcription units (TUs) used to express your...metabolic pathways. For many MoClo systems, the individual parts are reusable, saving time and money on ...array of DNA parts that can be assembled into individual TUs or very large (up to 20!) multi-TU constructs...