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We narrowed to 971 results for: lars

Showing: 41 - 60 of 971 results

  1. Cre-lox system

    Type
    Collection
    ...the sequence between the sites is deleted as a circular piece of DNA (and is not maintained). C) If the...13763 pTriEx-HTNC Cre fused to HIV-TAT - promotes cellular uptake of recombinant Cre Bacterial Rajewsky 13775...CMV-CFP;UBC-Cre25nt Cre with a 25 nucleotide extracellular vesicle targeting sequence and CFP UBC Mammalian...pAAV-hSyn-Cre-P2A-dTomato Cre and dTomato hSyn AAV Larsen 107787 AAV.TBG.PI.Cre.rBG Cre TBG AAV Wilson 107788...activates shRNA expression, removal of EGFP; See also similar plasmids pSico PGK GFP and pSico PGK puro Lentiviral...off shRNA expression, removal of EGFP; See also similar plasmids pSicoR PGK puro , pSicoR-Ef1a-mCh-Puro... to eGFP, removes dsRed. See article for other similar plasmids. Retroviral Clevers 15912 CTV Cre activates...

  2. Retrovirus Plasmids

    Type
    Collection
    ...LTR Description PI 1780 pBABE-neo largeTcDNA MoMLV Expression of Large T antigen for creation of immortalized...about retroviral plasmids at Addgene, including popular packaging, envelope, and transfer plasmids. Viral...Frequently asked questions (FAQ) about retrovirus Popular γ-Retrovirus Envelope and Packaging Plasmids Common...Envelope VSV-G-expressing envelope plasmid Kasparov Popular γ-Retroviral Transfer Plasmids Common γ-retroviral...

  3. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...characteristics and evolutionary similarity. These are grouped into two large classes based on the structure...: An Adaptive Immune System CRISPR (Clustered Regularly Interspaced Short Palindromic Repeat) sequences...made in just five years, with the skyrocketing popularity of CRISPR echoing that of RNAi and iPS cells....Cell . 159(3):647-6. PMID: 25307932 Gilbert LA, Larson MH, Morsut L, Liu Z, Brar GA, Torres SE, Stern-... WA, Weissman JS, Qi LS. 2013. CRISPR-mediated modular RNA-guided regulation of transcription in eukaryotes...

  4. Viral Production

    Type
    Collection
    ...immunogenic properties of the final product, particularly in large animal studies. Endotoxin contamination ...silver staining or SYPRO Ruby staining and the molecular weight and relative intensity of the viral capsid...lentiviral Rev responsive element (RRE) gene and the cellular ribonuclease P/MRP 30 kDa subunit (RPP30) as a...virus protocols . Browse our plasmid collection of popular lentiviral vectors ....

  5. Rett Syndrome

    Type
    Collection
    ...the world's largest biorepositories (Link opens in a new window) ATCC - the world's largest cell line repository...further downstream in the NID and C-terminus. A large number of mutations are known to cause Rett syndrome...accelerated our understanding of Rett syndrome at the molecular level. They are also an important tool for developing... PMID: 25801175 Tillotson and Bird. 2019. The Molecular Basis of MeCP2 Function in the Brain. J Mol Biol...

  6. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    ...Bygren, Lars O., et al. "Change in paternal grandmothers early food supply influenced cardiovascular mortality...PMC3929550. 3. Kaati, Gunnar, Lars O. Bygren, and Soren Edvinsson. "Cardiovascular and diabetes mortality determined...change in genotype. Epigenetic modifications play a large role in turning genes ‘off’ and ‘on’, signaling ...signaling which genes should be ‘read’ by the cellular machinery. This is how most cells in the body contain the...complex of DNA and histone proteins that compacts cellular DNA into chromosomes and reduces the risk of dangerous...the more compacted it becomes. This prevents the cellular machinery from physically interacting with the...Epigenetic changes have been discovered to play a large role in a variety of diseases, ranging from cancer...

  7. Caltech Systemic Capsids

    Type
    Collection
    ...serotype has been reported to occur by using the cellular receptor LY6A ( Huang et al., 2019 (Link opens...pAAV-hSyn-Cre-P2A-dTomato Syn Cre-expression Cre Larsen 140135 pAAV-EF1a-iCreV EF1a light-inducible recombinase... Cell type-biased: Neurons - AAV.CAP-B10 Brain vascular cells - AAV.CAP-Mac For rats: Broad tropism - ... Cell type-biased: Neurons - AAV.CAP-Mac Brain vascular cells - AAV9-X1.1, AAV1-X1 For mice: Broad tropism...AAV-PHP.eB with GRE or transgenic animal Brain vascular cells - AAV9-X1.1, AAV1-X1 Neurons - AAV.CAP-B10...

  8. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ...that can cause large genomic deletions. Compared to deletions generated by Cas9, large deletions created...Find plasmids for CRISPR-Cas and Cascade for large genomic deletions. CRISPR... While abundant in nature, Class 1 systems are largely underutilized compared to their Class 2 counterparts...

  9. Mammalian RNAi Tools

    Type
    Collection
    ...cleavage of the target. RNAi using siRNA follows a similar mechanism. Since it is pre-processed, it can bypass.... M., Abadi, S. J., & Gharagouzlo, E. (2017). Molecular mechanisms and biological functions of siRNA ....and assessment of gene knockdown . Methods in Molecular Biology, 629 , 141–158. PubMed (Link opens in ...., & Nemunaitis, J. (2009). siRNA vs. shRNA: Similarities and differences . Advanced Drug Delivery Reviews...

  10. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...Browse CRISPR pooled libraries for large-scale functional screens. Available for knockout, activation...representation. CRISPR screening experiments require large quantities of cells and therefore, may not be suitable...important to know which library best suits your particular experiment. Some important variables include:...given CRISPR library are unique to the genome of particular organism, and the library is only compatible ...Cherry Brie Pooled Library 170511 Knockout Mouse Tolar 3rd ~4 78,637 Mouse Chromatin Regulator Library ...

  11. Adenovirus Plasmids

    Type
    Collection
    ...Advantages of adenoviruses in research include their large packaging capacity (>8 kb), high titers, and high... For recombining shuttle plasmids; for use with large inserts Vogelstein 179201 pAd5-B6 ∆E3-GFP Adenoviral...Venus Wang Adenoviral Kits Plasmids containing modular inserts that span the human adenovirus serotype...1000000176 AdenoBuilder toolkit Plasmids contain modular inserts for seamless assembly of multiple mutations...

  12. University of Florida Serotype Testing Panel for the Eye and Brain

    Type
    Collection
    ...serotype also displays enhanced transduction of the trabecular meshwork following intracameral injection in ... Chamber Results in Efficient Transduction of Trabecular Meshwork in Mouse and Rat. PLoS One . Jun 8;10...AAV-mediated transduction and targeting of retinal bipolar cells with improved mGluR6 promoters in rodents...Intravitreal delivery of a novel AAV vector targets ON bipolar cells and restores visual function in a mouse model...

  13. Ras Pathway

    Type
    Collection
    ...nucleotide binding protein) family. GTPases serve as molecular switches that transmit signals within the cell...gene page are provided below. Color is used for clarity and does not indicate a specific relationship. ...Laboratory for Cancer Research Color is used for clarity and does not indicate a specific relationship. ...containing protein 1 SCRIB Scribbled planar cell polarity protein SHC SHC1 SHC2 SHC3 SHC4 SHC (Src homology...

  14. COVID-19 Resources

    Type
    Collection
    ... of the viral spike (S) protein to cellular receptors. The cellular receptor for SARS-CoV-2 and SARS-CoV...activity in neutralization assays and links to popular envelope and packaging plasmids. Ginkgo Bioworks...platforms to reliably detect nucleic acids at the atomolar level. For more information read Addgene's Blog...

  15. Allen Institute for Brain Science AAV Enhancer Collection

    Type
    Collection
    ...Mortrud M, Yao S, Siverts LA, Larsen R, Gore BB, Szelenyi ER, Trader C, Balaram P, van Velthoven CTJ, Chiang...using various delivery methods including intracerebroventricular (ICV) delivery in newborns, and retro-orbital...Juneau Z, Kalmbach B, Khem S, Kussick E, Kutsal R, Larsen R, Lee C, Lee AY, Leibly M, Lenz GH, Liang E, Lusk...

  16. CRISPR Plasmids and Resources

    Type
    Collection
    ...Cascade-Cas3 generates large genomic deletions. Insert NEW CRISPR transposases insert large fragments of DNA...CRISPR experiment. CRISPR 101 eBook : Based on our popular CRISPR 101 blog series, we've organized a comprehensive...

  17. Malate Dehydrogenase CUREs Community Collection

    Type
    Collection
    ...histidine-tagged constructs and routine protein and molecular biology techniques. Using a single protein with...species (e.g., human, watermelon, Streptomyces ) Subcellular compartment (chloroplastic, cytoplasmic, glyoxysomal...References for teachers Using Bioinformatics and Molecular Visualization to Develop Student Hypotheses in...

  18. Synthetic Biology - Overview

    Type
    Collection
    ...GreenGate Cloning System (Plant) MoClo Modular Cloning Modular PUF Assembly Kit Optogenetics Pertz Lab...Sensing and Signaling Browse plasmids related to intercellular signaling and environmental sensing. Strains...

  19. Antibody Plasmid Collection

    Type
    Collection
    ...Antibodies are large proteins made by the immune system that bind to an antigen, a particular part of a foreign...fragments that bind to the GCN4 peptide, from the popular SunTag system, fused to sfGFP for imaging. Learn...

  20. Microbiology Resources

    Type
    Collection
    ...MoClo Parts Kit : Molecular cloning for E. coli - Densmore Lab EcoFlex MoClo : Modular cloning for applications...Find plasmids to perform your experiment in a particular bacterial expression system. Fluorescent Protein...
Showing: 41 - 60 of 971 results