We narrowed to 997 results for: lars

... and tyrosine recombinases, based on sequence similarity and the amino acid residue used in the active... at once. They can mediate the integration of a large DNA payload containing an attB target site into ...the sequence between the sites is excised as a circular piece of DNA (and is not maintained). C) If the.... Using Cre or Flp as examples, here are a few popular recombinase-dependent gene expression strategies... some systems, such as Drosophila and mouse, particularly in proliferating cells. Expression may cause...controls such as Cre-only lines to account for cellular toxicity in your system. Using inducible Cre systems...Highlighted Plasmids Browse the tables below to find popular plasmids expressing your favorite site-specific...

...and Packaging Plasmids Popular Lentiviral Envelope and Packaging Plasmids Popular γ-Retrovirus Envelope...virus (such as HIV, murine leukemia virus, and vesicular stomatitis virus G) are packaged as replication-defective...infect permissive cells that express ACE2, the cellular receptor for SARS-CoV-2, in a single round of ...particles Murine leukemia-based retroviral particles Vesicular stomatitis virus particles See Addgene's Viral...packaging plasmids. Use the search bar to find a particular viral spike protein (MERS, CoV-1, CoV-2), modification...

...Assays Selection Validation Affinity reagents are molecular tools used in the lab to specifically and tightly...above are used to create hybridomas, which produce large amounts of a single antibody with little variation...antibody is made up of four protein chains: two large heavy chains and two small light chains. These four...In IHC, tissue sections are assayed and the extracellular matrix remains intact. The signaling molecule...enzyme. In ICC, cells have most or all of their extracellular matrix removed. Again, the signaling molecule... fluorophore colors in flow cytometry, but the largest panels used currently are in the mid-forties. When...conjugate or looking for antibodies validated for a similar assay type) and search again. After filtering, ...

...and transparency of the embryo and larvae, it is easy to culture large populations that are amenable to ...reporters, biosensors, and Cre expression plasmids. Large collections or kits are described below . ID Plasmid...Lab Lineage Tracing Cell lineage tracing in multicellular systems typically relies on genetic markers,... Lab Other Tools of Interest A few examples of popular tools to use in zebrafish, ranging from optogenetics...imaging platform to study lipid dynamics in vivo. Bimolecular fluorescence complementation (BiFC) tools - Marco...

... of the viral spike (S) protein to cellular receptors. The cellular receptor for SARS-CoV-2 and SARS-CoV...Addgene's COVID-19 collection of molecular tools and resources available for Coronavirus research. Plasmid...SARS-CoV-2 Plasmids Browse the tables below to find popular plasmids expressing genes encoding SARS-CoV-2 proteins...activity in neutralization assays and links to popular envelope and packaging plasmids. Ginkgo Bioworks...Syndrome Mice, Ts1Rhr . International journal of molecular sciences, 21 (6), 1947. https://doi.org/10.3390...

...physiological intracellular processes with genetically encoded fluorescent biosensors. Subcellular Localization...Localization Visualize organelles and subcellular structures with well-characterized marker proteins. In Vivo...Image with far-red or infrared probes to study molecular processes in organs and whole animals. Additional...Plasmids Use light to activate, inhibit, and control molecular pathways or cells. Chemogenetics Guide | Chemogenetics...

...that can cause large genomic deletions. Compared to deletions generated by Cas9, large deletions created...Find plasmids for CRISPR-Cas and Cascade for large genomic deletions. CRISPR... While abundant in nature, Class 1 systems are largely underutilized compared to their Class 2 counterparts...Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources. Find...

...Iterative Staining with Molecular annotations (PRISM) is a set of methods and molecular tools enabling high-throughput...Learn about PRISM, a set of molecular tools for high-throughput reconstruction of individual neurons ...co-delivery. Alternatively, plasmids can be combined at equimolar ratios and packaged together in one AAV preparation...self-correcting neuron tracing with nanoscale molecular context . bioRxiv 2025.09.26.678648; doi: https...

...characteristics and evolutionary similarity. These are grouped into two large classes based on the structure...: An Adaptive Immune System CRISPR (Clustered Regularly Interspaced Short Palindromic Repeat) sequences...made in just five years, with the skyrocketing popularity of CRISPR echoing that of RNAi and iPS cells....Cell . 159(3):647-6. PMID: 25307932 Gilbert LA, Larson MH, Morsut L, Liu Z, Brar GA, Torres SE, Stern-... WA, Weissman JS, Qi LS. 2013. CRISPR-mediated modular RNA-guided regulation of transcription in eukaryotes...

...immunogenic properties of the final product, particularly in large animal studies. Endotoxin contamination ...silver staining or SYPRO Ruby staining and the molecular weight and relative intensity of the viral capsid...lentiviral Rev responsive element (RRE) gene and the cellular ribonuclease P/MRP 30 kDa subunit (RPP30) as a...virus protocols . Browse our plasmid collection of popular lentiviral vectors ....

... packaging gene env This collection highlights popular second- and third-generation lentiviral packaging...production, and viral safety. Lentiviral Vector Guide Popular Lentiviral Transfer Plasmids Looking for lentiviral...protein. Ethan Abel 135991 pSLCAR-CD19-28z 3rd Modular CD28-CD3z CAR backbone. See Bloemberg et al. (2020...the MORF Collection . Feng Zhang Return to top Popular Lentiviral Packaging Plasmids ID Plasmid Generation... rev , and tat . Sergey Kasparov Return to top Popular Lentiviral Envelope Plasmids ID Plasmid Description...

...the world's largest biorepositories (Link opens in a new window) ATCC - the world's largest cell line repository...further downstream in the NID and C-terminus. A large number of mutations are known to cause Rett syndrome...accelerated our understanding of Rett syndrome at the molecular level. They are also an important tool for developing... PMID: 25801175 Tillotson and Bird. 2019. The Molecular Basis of MeCP2 Function in the Brain. J Mol Biol...

...serotype has been reported to occur by using the cellular receptor LY6A ( Huang et al., 2019 (Link opens...pAAV-hSyn-Cre-P2A-dTomato Syn Cre-expression Cre Larsen 140135 pAAV-EF1a-iCreV EF1a light-inducible recombinase... Cell type-biased: Neurons - AAV.CAP-B10 Brain vascular cells - AAV.CAP-Mac For rats: Broad tropism - ... Cell type-biased: Neurons - AAV.CAP-Mac Brain vascular cells - AAV9-X1.1, AAV1-X1 For mice: Broad tropism...AAV-PHP.eB with GRE or transgenic animal Brain vascular cells - AAV9-X1.1, AAV1-X1 Neurons - AAV.CAP-B10...

...cleavage of the target. RNAi using siRNA follows a similar mechanism. Since it is pre-processed, it can bypass.... M., Abadi, S. J., & Gharagouzlo, E. (2017). Molecular mechanisms and biological functions of siRNA ....and assessment of gene knockdown . Methods in Molecular Biology, 629 , 141–158. PubMed (Link opens in ...., & Nemunaitis, J. (2009). siRNA vs. shRNA: Similarities and differences . Advanced Drug Delivery Reviews...

...use with large inserts Vogelstein 1000000176 AdenoBuilder Toolkit Plasmids containing modular inserts ...dividing and non-dividing cells, and relative ease of large-scale production. In order to produce recombinant...This collection provides a curated selection of popular plasmids that can be used for adenoviral vector...

...Introduction to Viral Vectors Resources Viral vectors are popular tools for delivering genetic information to cells...long-term transgene expression. Retroviral vectors are popular for making stable cell lines or clinical use. Adenoviral...viruses infect cells in an organism and use the host cellular machinery to produce more virions. B) Viral plasmids...delivered to packaging cell lines and use their cellular machinery to generate viral vectors for research...

...The tetracycline (tet) system is one of the most popular choices for rapid, efficient, and reversible control...dox-inducible system). The outcome of Tet-On is similar to the natural TetR-tetO system, even though the...repressor. Tet-On has since become one of the most popular methods to induce gene expression in experimental...Highlighted Plasmids Browse the tables below to find popular empty backbones, expression vectors for transactivators... Top Other Tet Applications Explore some other popular applications of tetracycline systems or search ...

...serotype also displays enhanced transduction of the trabecular meshwork following intracameral injection in ... Chamber Results in Efficient Transduction of Trabecular Meshwork in Mouse and Rat. PLoS One . Jun 8;10...AAV-mediated transduction and targeting of retinal bipolar cells with improved mGluR6 promoters in rodents...Intravitreal delivery of a novel AAV vector targets ON bipolar cells and restores visual function in a mouse model...

...nucleotide binding protein) family. GTPases serve as molecular switches that transmit signals within the cell...gene page are provided below. Color is used for clarity and does not indicate a specific relationship. ...Laboratory for Cancer Research Color is used for clarity and does not indicate a specific relationship. ...containing protein 1 SCRIB Scribbled planar cell polarity protein SHC SHC1 SHC2 SHC3 SHC4 SHC (Src homology...

...genome. Addgene has a large collection of empty gRNA vectors. Explore our most popular plasmids for expression...Toolkit for Plants CRISPR Resources Addgene has a large selection of other CRISPR plasmids and resources...