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We narrowed to 111 results for: lentivirus

Showing: 41 - 60 of 111 results

  2. Protocols for Molecular Biology, Plasmid Cloning, and Viral Preps

    Type
    Protocol
    ...plasmid DNA to mammalian cells Lentivirus Production Produce lentivirus with a polyethyenimine (PEI) transfection...preparation Lentivirus Titration by ddPCR Use ddPCR to measure the physical titer of your lentivirus preparation...transfection protocol Fluorescence Titering Assay for Lentivirus Quantify virus using fluorescence measurements...measurements Colony Formation Titering Assay for Lentivirus Quantify virus by counting antibiotic resistant colonies...colonies Generating Stable Cell Lines with Lentivirus Genomically integrate your DNA sequence into a cell...

  4. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    ...always make more AAV than I think I need. Unlike lentivirus, AAV requires a higher number of viral particles...Addgene.org Learn how Addgene produces AAV and lentivirus Check out Addgene's virus protocols Find AAV ...

  5. Plasmid Collections

    Type
    Collection
    ...Luciferase Optogenetics Chemogenetics Viral Plasmids Lentivirus Adeno-associated Virus (AAV) γ-Retrovirus Adenovirus...

  6. Lentivirus ddPCR Titration

    Type
    Protocol
    ... of Lentivirus Vectors You may also like... Lentivirus Colony Formation Titration Assay Lentivirus Fluorescence... PCR (ddPCR) for lentivirus vector titration. Protocols... Protocols ddPCR Titration of Lentivirus Vectors ddPCR Titration...Assay AAV Titration by qPCR Addgene Viral Service Lentivirus Guide Virus Blog Posts Introduction This protocol...how to use droplet digital PCR (ddPCR) to titer lentivirus vectors. This protocol specifically uses primers...and surfaces with 10% bleach. Safety Warnings Lentivirus is generally considered biosafety level 2+. Please...average of several dilutions. Figure 1: ddPCR Lentivirus sample data, RRE positive (blue) and negative...

  8. Virus Protocol - Generating Stable Cell Lines

    Type
    Protocol
    ...Stable Cell Lines with Lentivirus Generating Stable Cell Lines with Lentivirus You may also like... Viral...Corning 3516 15 mL conical tubes, VWR 21008-216 Lentivirus preparation Appropriate antibiotic for selection...prior to use. Prepare a range of dilutions of the lentivirus in DMEM complete + 10 µg/mL polybrene. Note, ...titration. Mix the dilutions well. Dilution Volume of Lentivirus (μL) Volume of DMEM complete + 10 µg/mL polybrene...that the cells in the untransduced well (0 µL lentivirus, above) are dying. Perform regular media changes...lentiviral integration is random. Given the MOI of the lentivirus used, cells may also exhibit varying numbers ...

  10. Fluorescence Titering Assay

    Type
    Protocol
    ...Titering Assay Fluorescence Titering Assay for Lentivirus You may also like... Viral Vector Guides Virus...used in downstream applications. Safety Warnings Lentivirus is generally considered biosafety level 2+. Please...Corning 3516 15 mL conical tubes, VWR 21008-216 Lentivirus preparation Reagent Preparation DMEM Complete...on ice prior to use. Prepare dilutions of the lentivirus into DMEM complete containing 10 μg/mL polybrene...consider additional dilutions. Dilution Volume of Lentivirus Stock (μL) Volume of DMEM complete (μL) Volume...

  11. Colony Formation Titering Assay

    Type
    Protocol
    ...of the lentivirus into DMEM complete containing 10 μg/mL polybrene: Dilution Volume of Lentivirus or Previous...Titering Assay Colony Formation Titering Assay for Lentivirus You may also like... Viral Vector Guides Virus...Introduction This protocol can be used to titer lentivirus preparations that confer antibiotic resistance...Corning 3516 15 mL conical tubes, VWR 21008-216 Lentivirus preparation Reagent Preparation DMEM Complete...-Tip Different cells may be more sensitive to lentivirus or to polybrene, in which case this incubation...

  12. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ...transgene reactivation, residual expression Lentivirus Integrating High Low Efficient and Stable Genome...iPSC-transplanted mice. Cre-deletable or Tet-inducible lentiviruses address some of these concerns, but overall ...reprogramming factors driven by one promoter. While lentivirus has similar rates of reprogramming when compared...

  14. Adenovirus Plasmids

    Type
    Collection
    ...cell types with almost 100% efficiency. Unlike lentiviruses or other retroviruses , adenoviruses do not ...

  15. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    ...Jaenisch’s lab in plasmid form or as ready-to-use lentivirus. Figure 2: dCas9-Tet1 demethylates cytosines...with the former also available as ready-to-use lentivirus. Figure 3: dCas9-DNMT3A methylates cytosines...

  18. Important Considerations When Using AAVs

    Type
    Blog Post
    ...facilities (Biosafety Level-1). Other possible tools, lentiviruses for example, are not approved for use in vivo...Response: Compared to other viral vectors (e.g. Lentivirus, Adenovirus) AAVs cause the least amount of immune...
Showing: 41 - 60 of 111 results