Skip to main content
Addgene
Showing: 1 - 15 of 15 results

  1. Lentivirus Plasmids

    Type
    Collection
    ...Plasmids Lentiviral Plasmids Additional Resources: Lentivirus Guide Addgene's Viral Service Virus Biosafety...Plasmids Packaging Plasmids Transfer Plasmids Lentiviruses, a subset of retroviruses, are some of the most...and useful types of viruses used in research. Lentivirus can transduce both dividing and non-dividing ... considerations to evaluate when working with lentivirus: these viruses are based on HIV-1, which may ...Viral production Frequently asked questions about lentivirus use Glossary of commonly used lentiviral terms... and Packaging Plasmids One safety feature of lentivirus used in research is that the components necessary... that can be used with 2nd and 3rd generation lentivirus technologies. ID Plasmid Generation Type Description...

  2. Lentiviral Prep Service

    Type
    Collection
    ... Viral Vector Packaging Service Lentivirus Browse In-Stock Lentivirus Pooled CRISPR Libraries Cas9 Pooled...Ready-to-use lentivirus available from Addgene's viral vector packaging service. Browse constructs currently...Pooled Barcoding Libraries Control Addgene's lentiviruses are made from plasmids that are currently in ... viral preparation from the inventory below. Lentiviruses are distributed as purified viral particles ...non-targeting controls Puromycin Doench , Root Cas9 Lentivirus Cas9 viruses can be used to interrogate the genome...visualization via direct fluorescence. Morris Control Lentivirus Browse our list of available viruses based on...

  3. Plasmids for Stem Cell Research

    Type
    Collection
    ...Delivery Method Species Description Article PI Lentivirus Human Expression of human Oct4, Sox2, Klf4, Myc.... Nat Commun. 2018 Jul 6;9(1):2643. Otonkoski Lentivirus Human Expression of human Sox2, Nanog, Oct4, ...Science. 2007 Dec 21;318(5858):1917-20. Thomson Lentivirus Human Doxycycline-inducible expression of human...Stem Cell. 2008 Sep 11. 3(3):346-53. Jaenisch Lentivirus Human Single polycistronic lentiviral vector .... Stem Cells. 2009 May . 27(5):1042-9. Townes Lentivirus Human Single polycistronic lentiviral vector ...Stem Cells Dev. 2010 Aug;19(8):1221-9. Cibelli Lentivirus Human Single polycistronic, doxycycline-inducible...Pluripotency. Cell. 2015 Jul 16;162(2):412-24. Mikkelsen Lentivirus Human Expression of human Klf4, Oct4, c-Myc, ...

  4. Viral Production

    Type
    Collection
    ...viral production methods and quality control for lentivirus and AAV. ...AAV Production Titer Quality Control Resources Lentivirus Production Titer Quality Control Resources Overview...Browse our AAV vectors collection. AAV Data Hub Lentivirus Production Lentiviral preparations are produced... Cell culture medium (OptiPro SFM) containing lentivirus is first cleared by low-speed centrifugation ...preparations that have been stored at -80 °C and thawed. Lentivirus titration is performed using a 293T cell line...

  6. Viral Vectors

    Type
    Collection
    ...engineer various types of viruses. Lentivirus Plasmids | Guide Lentiviruses are versatile tools because of...Target Cell Genome Integration Immune Response Lentivirus Stable RNA <8 Kb 80-130 Dividing/Non-dividing...

  7. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ... Viral Vectors Lentivirus Oka Lab Lentiviral Vectors Baylor College of ...vectors and generating infectious virus. Protocol: Lentivirus Transfer Vector Production Protocol Lentiviral...

  8. Plasmid Collections

    Type
    Collection
    ...Luciferase Optogenetics Chemogenetics Viral Plasmids Lentivirus Adeno-associated Virus (AAV) γ-Retrovirus Adenovirus...

  9. Adenovirus Plasmids

    Type
    Collection
    ...cell types with almost 100% efficiency. Unlike lentiviruses or other retroviruses , adenoviruses do not ...

  10. Plan Your Experiment

    Type
    Collection
    ...lentiviral particles (for details about lentivirus, see our Lentivirus Guide ) Stable, tunable expression ...

  11. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...cells). Most CRISPR libraries require the use of lentivirus to deliver the gRNA/Cas9 to target cells. Therefore...Therefore, you must be equipped and authorized to use lentivirus in your lab. Once you have determined that CRISPR-based...

  12. CRISPR Guide

    Type
    Collection
    ...need to generate lentivirus containing the entire library (Figure 8D). This lentivirus will then be used...delivery to cells that are poorly transduced by lentivirus. Non-mammalian CRISPR libraries are also available...as liquid DNA, or in select cases, as pre-made lentivirus . DNA libraries are shipped at a concentration...need to either co-infect with a Cas9-producing lentivirus or use a Cas9-expressing stable cell line. After...

  13. Zhang Lab CRISPR Page

    Type
    Collection
    ...Addgene plasmids #61425 - #61427 are intended for lentivirus production : 61425 : lentiviral vector encoding...
Showing: 1 - 15 of 15 results