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We narrowed to 74 results for: mcherry expressing plasmid

Showing: 41 - 60 of 74 results

  1. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type
    Blog Post
    ...native expression levels. However, it is advisable to first express the fusion protein from a plasmid-based...instance, Lifeact-mTurquoise2 (Plasmid #36201) or mScarlet-tubulin (Plasmid #85045). We often use restriction...mTurquoise2, mEGFP, mNeonGreen, mScarlet(-I), and mCherry. Keep the linker sequence short In the early days...strategy (Sheridan, 2002). Avoiding protein over-expression artifacts The introduction of DNA that encodes...of protein within a cell and may lead to over-expression artifacts. This is an important downside of the...Download the fluorescent proteins 101 eBook Read our plasmids 101 blog series Resources on Addgene.org Browse...protein fusions Browse the fluorescent proteins plasmid kits ...

  2. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

    Type
    Blog Post
    ...fused to a reporter gene (e.g., mCherry) to easily detect opsin-expressing cells. The resulting fusion gene... about FLEx vectors Download our Plasmids 101 eBook Read our plasmids blog posts Resources on the Addgene...molecular biology reference Find Cre-lox plasmids at Addgene Browse plasmids at Addgene ...not naturally light-sensitive, so the selective expression of opsin genes in targeted cells makes it possible...How to get an opsin into the brain In order to express opsins within the brain, researchers inject genetically...relatively stable extra-chromosomally long-term expression and low ability to induce innate immune responses... promoters; however, those usually drive weak expression of downstream genes. Strong cell-specific promoters...

  3. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

    Type
    Blog Post
    ...Fluorescent Protein Resources Find Other Plasmids that Glow (Luciferase Plasmids) Find Optogenetics Resources ...tag with a serious drawback. The standard EGFP plasmid that used to be sold by Clontech and is in a freezer...solution is to restrict imaging analysis to cells expressing the lowest levels of the fusion protein. This... which cannot form heterodimers (i.e. EGFP and mCherry). These simple strategies can help you make better...

  4. Retrograde AAV viral preps

    Type
    Collection
    ...pAAV-hSyn-mCherry Syn mCherry Control Karl Deisseroth 114469 pAAV-CaMKIIa-mCherry CamKII mCherry Control...pAAV-Ef1a-mCherry EF1a mCherry Control Karl Deisseroth 114471 pAAV-Ef1a-fDIO mCherry EF1a mCherry, Flp-dependent...pAAV EF1a Nuc-flox(mCherry)-EGFP EF1a Color-flipping switch. Expresses NLS-mCherry in the absence of Cre...55634 pAAV-EF1a-mCherry-IRES-Flpo EF1a Flpo and physically-separate mCherry expression Recombinases Karl...Control Karl Deisseroth 50459 pAAV-hSyn-DIO-mCherry Syn mCherry, Cre-dependent Control Bryan Roth 59462 pAAV-CAG-tdTomato...AAV-pgk-Cre PGK Cre expression Recombinases Patrick Aebischer 55632 pAAV-Ef1a-mCherry-IRES-Cre EF1a Cre ...Cre and physically-separate mCherry expression Recombinases Karl Deisseroth 105540 pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40...

  5. Brain Armamentarium

    Type
    Collection
    ...Gradinaru 213945-AAV1 pAAV_BiSSTe4_ChR2_mCherry AAV construct expressing mCherry-tagged channelrhodopsin.... Wilson 213945-PHPeB pAAV_BiSSTe4_ChR2_mCherry AAV construct expressing mCherry-tagged channelrhodopsin...Gradinaru 213941-AAV1 pAAV_BiPVe3_ChR2_mCherry AAV construct expressing mCherry-tagged channelrhodopsin.... Wilson 213941-PHPeB pAAV_BiPVe3_ChR2_mCherry AAV construct expressing mCherry-tagged channelrhodopsin...Gradinaru 213937-AAV1 pAAV_BiPVe4_ChR2_mCherry AAV construct expressing mCherry-tagged channelrhodopsin.... Wilson 213937-PHPeB pAAV_BiPVe4_ChR2_mCherry AAV construct expressing mCherry-tagged channelrhodopsin.... Wilson 213915-AAV1 pAAV_BiLAMP5e3_ChR2_mCherry AAV construct expressing mCherry-tagged channelrhodopsin...

  6. Deisseroth INTRSECT Collection

    Type
    Collection
    ...Collections INTRSECT Plasmid Collection INTRSECT Plasmid Collection INTRSECT Implementation Plasmids References ...Find intersectional expression plasmids that combine multiplexed recombinases, including Cre, Flp, and...Example of viral targeting of cells expressing Cre AND Flp, here expressed through a double transgenic animal...recombinase-expressing mouse lines 2–9 and combinations of transgenic recombinase-expressing animal lines...novel INTSRECT plasmids for groups interested in producing their own INTRSECT plasmids. Online spreadsheet... pAAV-Ef1a-mCherry-IRES-Cre None Yes 55637 pAAV-EF1a-Flpo None Yes 55634 pAAV-EF1a-mCherry-IRES-Flpo None...137132 pAAV-Ef1a-Con/Fon-mCherry Cre AND Flp Yes 137133 pAAV-Ef1a-Con/Foff 2.0-mCherry Cre AND NOT Flp FRT/...

  7. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    ...CRISPR Guide Find Plasmids for Cas9 Purification Find CRISPR Plasmids for Mammalian Expression ...editing. How to use Addgene plasmids for Easi-CRISPR You can use Addgene plasmids to create a repair template...of Addgene plasmid options for templates - see the example at right using blue flame plasmid pmScarlet_...desired insert (e.g., mScarlet).   Plasmid ID Plasmid Name Tags 69090 pMJ915 MBP (N ...pre-CRISPR was very arduous. First, you’d construct a plasmid with your desired changes, long homology arms and...template! If you have a plasmid with your desired insertion feature (e.g., a fluorescent protein), you can...cassettes, such as fluorescent proteins (EGFP, mCherry etc.), recombinases (Cre, Flp etc.), and tetracycline...

  8. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ... on the plasmid. gRNA plasmids that do not co-express a Cas protein require a separate plasmid that does...Vectors CRISPR Plasmids: Empty gRNA Expression Vectors Browse CRISPR Plasmids By Function Genome Editing Cut...maintained by the Doudna Lab . Plasmid Expression System Cloning Enzyme Co-expressed Cas9 Cas9 System Selection...CBh-Cas9-T2A-mcherry-P2A-Ad4E1B 64221 Mammalian see plasmid page yes, cut S. pyogenes mCherry Kuhn pU6-(...CBh-Cas9-T2A-mcherry-P2A-Ad4E4orf6 64222 Mammalian see plasmid page yes, cut S. pyogenes mCherry Kuhn AAV:...can be used to express multiple gRNAs from a single plasmid with or without expression of Cas9. Highlighted.... Can express Cas9 with up to 3 gRNAs. Frew pSQT1313 Mammalian Single plasmid for the expression of multipe...

  9. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...SepOTSλ Plasmid 68291 SepOTSκ Plasmid 68290 SepOTSι Plasmid 68289 SepOTSθ Plasmid 68288 SepOTSη Plasmid 68287...iSPI_pSer_Subpool#1 Plasmid 112031 pNAS1b split mCherry 14-3-3β ctrl Plasmid 112030 pNAS1b split mCherry NEDD4 WW2... ctrl Plasmid 112029 pNAS1b split mCherry NEDD4 WW2 pos ctrl Plasmid 111883 pNAS1b split mCherry NEDD4...NEDD4-2 WW2 Plasmid 111882 pNAS1b split mCherry NEDD4 WW2 Plasmid 111881 pNAS1b split mCherry 14-3-3σ Plasmid...S218TAG Plasmid 68300 MBP-MEK1 Plasmid 68299 GFP E17TAG/Q157TAG Plasmid 68298 GFP S2TAG/E17TAG Plasmid 68297...GFP Q157TAG Plasmid 68296 GFP S2TAG Plasmid 68295 GFP E17TAG Plasmid 68294 SepOTSν Plasmid 68292 SepOTSλ...68287 SepOTSζ Plasmid 68286 SepOTSϵ Plasmid 68285 SepOTSδ Plasmid 68284 SepOTSγ Plasmid 68283 SepOTSβ Plasmid...

  10. Brain Initiative Collection

    Type
    Collection
    ...driven KOR DREADD expression. Bryan Roth 75033-AAV1 pAAV CD68-hM4D(Gi)-mCherry Expression of hM4D(Gi) using... 83898-AAV1 pAAV-mDlx-ChR2-mCherry-Fishell-3 ChR2-mCherry fusion expression in forebrain GABA-ergic interneurons... 83898-AAV9 pAAV-mDlx-ChR2-mCherry-Fishell-3 ChR2-mCherry fusion expression in forebrain GABA-ergic interneurons...83898-AAVrg pAAV-mDlx-ChR2-mCherry-Fishell-3 ChR2-mCherry fusion expression in forebrain GABA-ergic interneurons...Chemogenetics expression plasmid Scott Sternson 123308-AAV9 pAAV-hSyn-GRAB_NE1m Expresses the genetically-encoded...135634-AAV1 pAAV-S5E2-ChR2-mCherry AAV vector to drive the expression of ChR2-mCherry in PV cortical interneuronsunder...135634-AAV9 pAAV-S5E2-ChR2-mCherry AAV vector to drive the expression of ChR2-mCherry in PV cortical interneuronsunder...

  11. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...mammalian expression plasmid for cloning untagged proteins pcDNA3.1-mCherry - Expresses mCherry tag in mammalian...GFP for mammalian expression pLV-mCherry - 3rd gen lentiviral plasmid expressing mCherry pBad-EBFP2 - Bacterial...Species-Specific Expression If you want to drive expression of your favorite gene, you will need a plasmid with...EF1a, CAG, Ubc Transient expression - Gradia Lab plasmids for mammalian expression, Ligation Independent ...TEV cleavage Transient expression - Gradia Lab plasmids for bacterial expression, Ligation Independent ... Expression - Rubin lab plasmids for Drosophila expression pMT-puro - Inducible...tagging vectors in budding yeast pHT101-mCherry - Expresses mCherry tagged fusion proteins in worms ins:MCS...

  12. Bikard Lab - CRISPR Repression Collection

    Type
    Collection
    ...From the Bikard Lab, CRISPR plasmid collection for modulating expression levels of two targets independently... can obtain a range of expression levels. This collection comprises 20 plasmids, each carrying two CRISPR...binds to mcherry with either 0, 11, 18, or 20 matching nucleotides. Together, these plasmids allow to ...range of expression levels for these two reporters simultaneously. By fusing sfGFP and/or mCherry to genes... modulate expression of two genes without cross‐talk. (A) Schematic of the strain expressing two reporters...the two reporters, mCherry and sfgfp in this case, can be controlled using a plasmid‐borne CRISPR array...Repression Collection Bikard Lab CRISPR Repression Plasmids You may also like... CRISPR Guide CRISPR Protocols...

  13. Control AAV Preps

    Type
    Collection
    ...Constitutive 5 Hongkui Zeng 58909 pAAV-GFAP104-mCherry GFAP104 mCherry Constitutive 5 Edward Boyden 59462 pAAV-CAG-tdTomato...2 Marcella Patrick 114469 pAAV-CaMKIIa-mCherry CaMKIIa mCherry Constitutive 1, 2, 5, 8, 9, rg*, PHP.eB...PHP.eB Karl Deisseroth 114470 pAAV-Ef1a-mCherry EF1a mCherry Constitutive 1, 2, 5, 8, 9, rg*, PHP.eB Karl ...Karl Deisseroth 114472 pAAV-hSyn-mCherry hSyn mCherry Constitutive 1, 2, 5, 8, 9, rg* Karl Deisseroth 117382...Zhang 20299 pAAV-EF1a-double floxed-mCherry-WPRE-HGHpA EF1a mCherry Cre dependent 9 Karl Deisseroth 27056...112677 pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP EF1a NLS-mCherry or nls-EGFP Cre dependent 1, 2, 5, ...8, 9, rg* Bryan Roth 50459 pAAV-hSyn-DIO-mCherry hSyn mCherry Cre dependent 1, 2, 5, 8, 9, rg* Bryan Roth...

  14. Bacterial Expression Systems

    Type
    Collection
    ...selection of plasmids and tags for protein purification. The pTD Plasmid Series contains plasmids suitable...and expression species. The New England Biolabs Cell-Imaging Plasmid Collection contains plasmids for ...turn on expression only at a specific time. This collection contains plasmids whose expression levels ...Find bacterial expression plasmids that can be used for protein purification, protein visualization, ...controlled gene expression, reporter plasmids and genome engineering of bacteria. Plasmid...Bacterial Expression Systems Bacterial Expression Systems Protein Purification Protein Visualization Protein... Protein Interactions Controlled Expression Reporter Plasmids Genome Engineering You may also like… Genetic...

  15. Caltech Systemic Capsids

    Type
    Collection
    ...pAAV-CaMKIIa-mCherry CamKIIa mCherry Control Deisseroth 114470 pAAV-Ef1a-mCherry EF1a mCherry Control Deisseroth...pAAV-hSyn-DIO-hM3D(Gq)-mCherry Syn Activator, Cre-dependent DREADD Roth 44362 pAAV-hSyn-DIO-hM4D(Gi)-mCherry Syn Inhibitor...pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 Syn Cre-EGFP expression Cre Wilson 105550 pAAV.GFAP.Cre.WPRE.hGH GFAP Cre-expression Cre Wilson 107738...preparations were produced with the pUCmini-iCAP-PHP.eB plasmid (Addgene #103005) . Note on PHP.eB tropism and ...Activator C1V1 Dimidschstein 135634 pAAV-S5E2-ChR2-mCherry E2 regulatory element Activator ChR2 Dimidschstein...107738 pAAV-hSyn-Cre-P2A-dTomato Syn Cre-expression Cre Larsen 140135 pAAV-EF1a-iCreV EF1a light-inducible...rAAV-hI56i-minBglobin-iCre-4X2C-WPRE3-BGHpA minBG iCre expression iCre Ting 199775 AiP13778 - pAAV-AiE0450h-minBG-iCre...

  16. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...selectable marker, and whether the plasmid contains Cas9. ID Plasmid Expression System Promoter Guide RNA Type...Drosophila ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Plant ID Plasmid Gene...RNA-H1-nick sgRNA-mCherry Mammalian, AAV hU6 + H1 pegRNA + nicking sgRNA No mCherry Hyongbum Kim 177180...pDAS12069_U6-pegRNA-mCherry Mammalian hU6 pegRNA BpiI for pegRNA, Esp31 for PBS-RT No mCherry Ervin Welker 177181...selection of CRISPR plasmids and resources. Find more CRISPR functions along with plasmids categorized by ...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...prime editor plasmids for (search and replace) genome editing. CRISPR...

  17. Recombinases AAV Preps

    Type
    Collection
    ...which can be used to control gene expression. See our Cre-lox Plasmids page for more information about ..., 9 James M. Wilson 55632 pAAV-Ef1a-mCherry-IRES-Cre EF1a mCherry (not a fusion tag) 8, rg* Karl Deisseroth... 9 James M. Wilson 107312 AAV-hSyn-mCherry-P2A-Cre-WPRE Syn mCherry 1 Hui Yang 107738 pAAV-hSyn-Cre-P2A-dTomato... rg* Ian Wickersham 55634 pAAV-EF1a-mCherry-IRES-Flpo EF1a mCherry (not a fusion tag) 1, rg* Karl Deisseroth...We provide high quality AAV preps from select plasmids in the repository. Browse our AAV collection of...retrograde serotype, produced with the AAV retro helper plasmid from the Karpova and Schaffer labs. See the Retrograde...options. Select from a vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need....

  18. Retrovirus Plasmids

    Type
    Collection
    ...Gamma-Retroviral Plasmids Gamma-Retroviral Plasmids Transfer Plasmids Packaging Plasmids Envelope Plasmids Additional...MSCV Plasmid for transgene expression with luciferase expression. Scott Lowe 9044 pMIG MSCV Plasmid for... shRNA expression with GFP expression. See plasmid 8452 for puromycin resistance. William Hahn 63704 pRetroX...for transgene expression with GFP expression. See plasmid 12282 for the addition of WRE, a post-transcriptional...miRNA expression. Chang-Zheng Chen 52114 pMSCV-IRES-mCherry FP MSCV A bicistronic retroviral plasmid with... IRES-mCherry. Dario Vignali 27995 TtRMPVIR CMV/MSV Tet-regulated expression of shRNA. Expresses rtTA....additional plasmids in this toolkit. Scott Lowe 64865 pLncEXP CMV/MSV lncRNA expression plasmid. Lei Sun...

  19. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...collection of SARS-CoV-2 spike plasmids, packaging plasmids, and reporter plasmids for viral pseudotyping with...Addgene's Viral Plasmids and Resources page for more information. Plasmids Expressing Spike and Spike ...Accessory Plasmids Envelope and Packaging Plasmids Popular Lentiviral Envelope and Packaging Plasmids Popular...Envelope and Packaging Plasmids Reporter Plasmids A few examples of reporter plasmids that can be used for...2 Pseudotyped Virus SARS-CoV-2 Spike Plasmids Accessory Plasmids Other Resources Mechanisms of SARS-CoV...circulating variants. The table below lists expression plasmids that are suitable for generating pseudotyped...activity are listed below: ID Plasmid Description PI 44965 NL4-3 mCherry Luciferase Lentiviral dual reporter...

  20. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...72264 Express mCherry and puromycin resistance gene from the CMV promoter pCDH-CMV 72265 Express gene ...Vector Guide Find more plasmids in our Lentiviral Plasmids and Adenoviral Plasmids collections Addgene Blog...reporter plasmids to test whether or not you've efficiently generated infectious virus, plasmids expressing...-EF1-DIO-copGFP 72253 Expresses copGFP under EF-1 promoter when Cre is expressed by another vector pCDH-EF1...pCDH-EF1-DIO-tdTomato 72254 Expresses tdTomato under EF-1 promoter when Cre is expressed by another vector pCDH-CB-iCre-P2A-tdTomato-T2A-Puro...pCDH-CB-copGFP-T2A-iCre 72256 Expresses copGFP and iCre under from the CB promoter pCDH-CB-iCre 72257 Express iCre under ...pCDH-CB-FLPe-P2A-tdTomato 72259 Expresses FLPe and tdTomato from the CB promoter pCDH-EF1-Fon-copGFP 72260 Expresses copGFP from...
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