...factor and promoter pairs referred to as binary systems. Binary systems are genetic tools that consist ...repressible element to this binary system (making this a repressible binary system). The GAL80 repressor...default ON to an OFF state, as well as repressible binary systems. In recent years, a number of more complex...consumption phase of yeast culture. Repressible Binary Systems GAL4/UAS In Drosophila or development studies... gene expression across development. One common binary system is the GAL4/UAS system isolated from yeast... Valle Rodriguez et al.   LexA/lexAop A second binary system is LexA/lexAop, developed by Lai and Lee...QS and quinic acid. From Riabinia et. al. Using Binary Systems Together You may be wondering, “why is ...

...Agrobacterium binary vectors Vectors designed for use in Agrobacterium are termed binary vectors because...depositor Indu Maiti has created a new and versatile binary Ti vector for both transient and stable gene expression...and E. coli. Like plasmids used in other systems, binary vectors are cloned and prepped in E. coli. However...co-cultivation with the target plant species. Thus, binary vectors contain two origins of replication, one...into a single plasmid, so most first-generation binary vectors are quite large (>10 kb.) Since transformation...scientists want to transfer large genes, smaller binary vectors with a larger cloning capacity would be...be very useful: enter pSIM24! To build a better binary vector, Sahoo et al. started with a vector commonly...

...expression cassettes, which can be assembled into the binary CRISPR/Cas9 vectors in one round of cloning by ...components are then assembled into a single T-DNA binary vector of choice through Gateway recombination....

...many base editors are designed to work in a very narrow window proximal to the PAM sequence, some base ..., J., . . . Koonin, E. V. (2015). An updated evolutionary classification of CRISPR–Cas systems. Nature...., Mohanraju, P., Fedorova, I., Kneppers, J., DeGennaro, E. M., Winblad, N., Choudhury, S. R., Abudayyeh...

...toxicity of CcdB.   Figure 1: Image from Bernard, P., et al. showing the pKIL 18/19 vectors and ...a guest blog entry of your own! References 1. Bernard, P. “Positive Selection of Recombinant DNA by CcdB...1995 Mar;15(6):1031-7. PubMed PMID: 7623659. 3. Bernard, P., et al. “Positive-selection vectors using the...1994 Oct 11;148(1):71-4. PubMed PMID: 7926841. 4. Bernard, P., et al. “The F plasmid CcdB protein induces... Dec 5;234(3):534-41. PubMed PMID: 8254658. 5. Bernard, P. and Couturier, M. “Cell killing by the F plasmid...

...AAV Zeng 140284 pCAG-Cre-iBSD Cre CAG Mammalian Miyanari 140760 Sox1(2A-Cre) Cre; Targeting vector for ...See article Multibow constructs Multibow uses a binary code in which reporters are initially OFF and then...

...growing amount of interdisciplinary research while at the same time increasing interdisciplinary citations in... solutions to complex challenges. As a cross-disciplinary researcher, the challenge of keeping up on studies...interactive chart where you can see the yearly interdisciplinarity index of research for single or multiple ...2009): 858-862. 2. Van Noorden, Richard. "Interdisciplinary research by the numbers." Nature 525.7569 ...

...some public speaking and a bit of video work for webinars (get tips for your next science talk). I do illustrations...website quite a bit.” Out of all of these tasks, webinars are Hans’ favorite thing to work on. “It’s one...someone to take over hosting them. Since then, webinars make up a larger part of Hans’ workload. “I am...getting abstracts and plans together for my next 4 webinars—I have them stacked close together for the next...

...Did you catch our April AAV webinar with Tim Miles, PhD, Director of the CLOVER Center at CalTech? If...are sold in packages of 10…)  If you missed the webinar, you can watch the presentation and Q&A session...considering the need for some manner of polyA. In this scenario, you may be best served by a dual vector, split...recent review.      And that's a wrap on our AAV webinar! Special thanks to our panel of experts, Tim Miles...

...minimally-invasive, Frances Arnold’s lab and Viviana Gradinaru’s lab used machine learning to design new channelrhodopsins... To overcome these challenges, the Arnold and Gradinaru Labs developed a machine-learning model to help...their success in vivo. Therefore, the Arnold and Gradinaru Labs expressed ChRgers in mice to see if they ...Bedbrook CN, Yang KK, Robinson JE, Mackey ED, Gradinaru V, Arnold FH (2019) Machine learning-guided channelrhodopsin...

... Having incorporated a discovery-based lab on planarian regeneration into one of my own courses, I was...required for stem and progenitor cell maintenance in planarians in Developmental Biology (Kimball et al., 2020...undergraduates conducting NIH- and NSF-funded research on planarian flatworms both in and out of the classroom.   ...required for stem and progenitor cell maintenance in planarians. Developmental Biology. 457:119-127. Knutson,...

...career scientists are those in the Young Scientist Seminar Series. Watch how these speakers talk without looking... slides or notes. Listen to their stories and narratives (see below “Tell a story”) and note how they ...the point.   What makes a story? A story is a narrative connecting cause and effect. Stories can and should...obstacles. Take time to consider the order of your narrative. Scientists do this all the time, you just need...

...dirty clothing, lab wears, human speech near the laminar airflow, the human scalp, sneezing, coughing, etc...while talking or pipetting) during working in the laminar airflow are also among the leading causes of contamination...the required equipment and reagents inside the laminar airflow before and while you are working. Perform...with every subculture. Equipment Fumigate the laminar airflow and the lab facility periodically. The ...

... Care Unit, the Pulmonary Consultation Service, the Alpha-1 Center, and the Pulmonary outpatient clinic... therapy, and tissue engineering. How the multidisciplinary nature of the Center for Regenerative Medicine...

...this goal could involve participating in interdisciplinary seminars, such as those in bioinformatics or chemistry...lies within a biological lab. Engaging in such seminars exposes you to discussions beyond your immediate...

...AAV genome sequencing (Tai 2019; Lecomte 2015; Maynard 2019). Unfortunately, at present SMRT sequencing...preparation approaches described previously require a preliminary double stranding step. The double stranding step... 4:e260 . https://doi.org/10.1038/mtna.2015.32 Maynard LH, Smith O, Tilmans NP, Tham E, Hosseinzadeh S...

...Kohara et al., 2014; plasmids at Addgene). The Gradinaru Lab at the California Institute of Technology ...Lois, C., Mazmanian, S.K., Deverman, B.E., and Gradinaru, V. (2017). Engineered AAVs for efficient noninvasive..., J.G., Wohnoutka, P., Gerfen, C.R., Koch, C., Bernard, A., Dang, C., Jones, A.R., and Zeng, H. (2014)... PMID: 25240284. Wickersham, I.R., Lyon, D.C., Barnard, R.J.O., Mori, T., Finke, S., Conzelmann, K.-K....

... new search tool, are the results of this multidisciplinary effort and cross-team collaboration (thanks...scoping mechanism that allows category-specific, narrow searches. We defined three top level categories... terms in a query in a way that allows users to narrow down search results by adding more terms.  Weighting...

...computational processing with biological protocols and revolutionary physical approaches. However, with each new ...makerspace networks and training users via workshops, seminars, or hackathons greatly increases accessibility...brings stronger networking across laboratory and disciplinary boundaries and can allow for innovative organizations...

...considerations will help you narrow down which antibody format to use for your specific scenario.    References and...Fab fragments can be used as a block in similar scenarios. By applying unconjugated Fab fragments that are...