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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 292 results for: nif

Showing: 41 - 60 of 292 results

  1. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    ...development happening at Addgene under the hood. A significant portion of our development may not be visible... been updated in several years) or they make significant changes that make it incompatible with our software...software from Python 2.7 to Python 3.5. There are significant changes to the language and it took us a total...

  2. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    ... the cytoplasm? Or more troubling, what if a significant fraction of the fusion protein molecules fail...resident proteins within these compartments undergo significant post-translational modifications including glycosylation...University, did his postdoctoral fellowship with Jennifer Lippincott-Schwartz at the National Institutes...

  3. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    ...vastly expanded axolotl research. One of the most significant advances is the sequencing of the entire axolotl...research providing insight into some of the most significant and fundamental biological processes. Many of...widespread utilization, Arabidopsis has made significant contributions to understanding plant and crop...

  4. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    ... to keep in mind, but likely will not have a significant impact on your data. You are trying to generate...selectively sequenced to determine if there are significant editing events, serving as a proxy for total ... to effectively control for off-targets and significantly decrease the probability of artifacts. Off-target...

  5. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type
    Blog Post
    ...that their knowledge and skills had improved significantly when compared to their pre-program self-assesment...improvements, not necessarily quantifiable, but always significant. Doubts, fears, insecurities… all shrink when...generations of professionals has made possible a significant qualitative and quantitative expansion in the...

  7. Plasmid Cloning by PCR (with Protocols)

    Type
    Protocol
    ...recipient plasmid + insert plate should have significantly more colonies than the recipient plasmid alone...recipient plasmid self-ligation, you will see significantly more colonies when you add ligase. If you do...

  8. Addgene is Stopping Use of X (formerly Twitter)

    Type
    Blog Post
    ... suggestions in the comments.  X has moved significantly away from the functionality and features that...this reason, our timeline has accelerated. We significantly reduced posting on November 20, 2023, and as...

  9. Overwhelmed? Take a Break With Our 5 Favorite Science Comics

    Type
    Blog Post
    ... have your first cold of the season like I do, sniffle) ourselves back into our regular post-holiday routines...Researchers in Taiwan showed that a laughter program significantly decreased immunological markers of stress in...

  11. DIY DNA Ladders from Penn State University

    Type
    Blog Post
    ...worth of ladder. The Penn State ladders are significantly cheaper. When using a homemade plasmid prep ...increases the costs to $20-30 per ladder: still significantly cheaper than commercial ladders. Are you interested...

  12. What's New in CRISPR - Winter 2018

    Type
    Blog Post
    ...genome editing toolkit New Cas protein identified Jennifer Doudna’s lab recently identified Cas14, a family... Jennifer Tsang...

  13. Keeping Up With CRISPR/Cas9

    Type
    Blog Post
    ...there's a pretty good reason for that. Lately, significant advances in the understanding and application... work. Last month, the Lawrence Berkeley Lab's Jennifer Doudna and Eva Nogales presented the first detailed...

  14. What's New in CRISPR - Spring 2019

    Type
    Blog Post
    ...a bioinformatics and a screening approach, the Jennifer Doudna lab identified three different Cas12a inhibitors... Jennifer Tsang...

  16. What's New in CRISPR - September 2019

    Type
    Blog Post
    ...active in E. coli and human cells Two years ago, Jennifer Doudna’s lab discovered a new Cas protein, CasX... Jennifer Tsang...

  17. What's New in CRISPR - March 2020

    Type
    Blog Post
    ...fluorescently labeled piggyBac vectors to deliver uniform and sustained expression of multiplexed gRNAs in... Jennifer Tsang...

  20. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

    Type
    Blog Post
    ...IgGs and your primary antibody, resulting in significant background staining (Figure 1A). Not all tissues...Figure 2B) Some labs have found that you can significantly decrease background staining by mixing your ...
Showing: 41 - 60 of 292 results