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Showing: 41 - 60 of 270 results
  1. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    ... to keep in mind, but likely will not have a significant impact on your data. You are trying to generate...selectively sequenced to determine if there are significant editing events, serving as a proxy for total ... to effectively control for off-targets and significantly decrease the probability of artifacts. Off-target...
  2. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type
    Blog Post
    ...that their knowledge and skills had improved significantly when compared to their pre-program self-assesment...improvements, not necessarily quantifiable, but always significant. Doubts, fears, insecurities… all shrink when...generations of professionals has made possible a significant qualitative and quantitative expansion in the...
  3. Gibson Assembly Protocol

    Type
    Protocol
    ...homology region. Hairpins in this region can significantly reduce the efficiency of two homologous ends...gel to check for size and yield. If there are significant amounts of undesired product, gel-purify DNA ...
  4. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ...published Aug 16, 2016 and last updated Aug 6, 2020 by Jennifer Tsang. When we talk about CRISPR applications,...mixed population of edits, ABEs do not display significant A to non-G conversion at target loci. The removal...Has both cytosine and adenine base editing Jennifer Tsang contributed to updating this article. References...
  5. Plasmid Cloning by PCR (with Protocols)

    Type
    Protocol
    ...recipient plasmid + insert plate should have significantly more colonies than the recipient plasmid alone...recipient plasmid self-ligation, you will see significantly more colonies when you add ligase. If you do...
  6. Addgene is Stopping Use of X (formerly Twitter)

    Type
    Blog Post
    ...welcome suggestions in the comments.  X has moved significantly away from the functionality and features that...this reason, our timeline has accelerated. We significantly reduced posting on November 20, 2023, and as...
  7. Overwhelmed? Take a Break With Our 5 Favorite Science Comics

    Type
    Blog Post
    ... have your first cold of the season like I do, sniffle) ourselves back into our regular post-holiday routines...Researchers in Taiwan showed that a laughter program significantly decreased immunological markers of stress in...
  8. DIY DNA Ladders from Penn State University

    Type
    Blog Post
    ...worth of ladder. The Penn State ladders are significantly cheaper. When using a homemade plasmid prep ...increases the costs to $20-30 per ladder: still significantly cheaper than commercial ladders. Are you interested...
  9. What's New in CRISPR - Winter 2018

    Type
    Blog Post
    ...genome editing toolkit New Cas protein identified Jennifer Doudna’s lab recently identified Cas14, a family... Jennifer Tsang...
  10. Keeping Up With CRISPR/Cas9

    Type
    Blog Post
    ...there's a pretty good reason for that. Lately, significant advances in the understanding and application... work. Last month, the Lawrence Berkeley Lab's Jennifer Doudna and Eva Nogales presented the first detailed...
  11. What's New in CRISPR - Spring 2019

    Type
    Blog Post
    ...a bioinformatics and a screening approach, the Jennifer Doudna lab identified three different Cas12a inhibitors... Jennifer Tsang...
  12. What's New in CRISPR - March 2020

    Type
    Blog Post
    ...fluorescently labeled piggyBac vectors to deliver uniform and sustained expression of multiplexed gRNAs in... Jennifer Tsang...
  13. What's New in CRISPR - September 2019

    Type
    Blog Post
    ...active in E. coli and human cells Two years ago, Jennifer Doudna’s lab discovered a new Cas protein, CasX... Jennifer Tsang...
  14. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

    Type
    Blog Post
    ...IgGs and your primary antibody, resulting in significant background staining (Figure 1A). Not all tissues...Figure 2B) Some labs have found that you can significantly decrease background staining by mixing your ...
Showing: 41 - 60 of 270 results