We narrowed to 67 results for: nts
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Targeted Mutagenesis with EvolvR
TypeBlog Post...initial version of EvolvR has an editing window of ~17 nt from the nick site, which was expected since the ... -
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CRISPR 101: Non-Homologous End Joining
TypeBlog Post...doi.org/10.1016/j.cell.2013.08.021 Waters CA, Strande NT, Pryor JM, Strom CN, Mieczkowski P, Burkhalter MD... -
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Hot Plasmids - October 2020
TypeBlog Post...introduce the small wrmScarlet11 which is less than 60 nt in length to tag the target protein. The team validated... -
Tips for Using BLAST to Verify Plasmids
TypeBlog Post...default nucleotide database “Nucleotide collection (nr/nt)” as it contains a composite of GenBank, EMBL, DDBJ... -
xCas9: Engineering a CRISPR Variant with PAM Flexibility
TypeBlog Post...mutations, also has a strict editing window about 15 nt upstream of the PAM. Even if there’s a PAM near your... -
Design Tips for Prime Editing
TypeBlog Post... binding site, starting with a length of about 13 nt (Anzalone et al., 2019). Primer binding sites with... -
INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons
TypeBlog Post... 1A, top): A CRISPR RNA (crRNA) comprising a 32 nt spacer sequence specifying the target site. Four ... -
Fluorescent Proteins 101: Aptamer Fluorophores
TypeBlog Post... in vivo, the Jaffrey lab used SELEX to find a 98 nt RNA aptamer that would bind the fluorogen and increase...