We narrowed to 1,015 results for: nts

...antibody components into plasmids and expressing these in bacteria, mammalian cells, yeast, plants, or insect...size. These recombinant antibody fragments can be used in experiments such as immunoprecipitation and ...antibodies as Fab' fragments, isotype-switched chimeric antibodies, and Fc-silent mutants. Functional diversification...microscopy. Two commonly used plasmid-based antibody fragments are scFvs and single-domain antibodies (also known..., scientists often use plasmids encoding scFv fragments that bind to the GCN4 peptide, from the popular...the generation of chicken monoclonal antibody fragments by phage display. J Immunol Methods. 2000 Aug ...dimerisation and detection of recombinant antibody fragments in the form of scFvs A simple vector system to...

...proteins available, and researchers can adapt these experiments for different organisms. This page provides a...CRISPR in your research and setting up CRISPR experiments. We will focus on making single edits using CRISPR...using CRISPR in other organisms. As with most experiments, you first need to have a biological question...you are ready to start designing the specific components (Cas enzyme and guide RNA) for your experiment...genetic manipulations require different CRISPR components. Selecting the genetic manipulation you plan ...plan to perform will help you narrow down which components you’ll need for a given experiment. In the table... window from the PAM sequence, and base editor variants often have different optimal editing windows. ...

...luciferase a popular tool for scientists. Regulatory elements such as promoters, enhancers and untranslated ...secreted and more stable, enabling time course experiments, but is too dim for some applications. NanoLuc...Creating ORF-Firefly luciferase gene fusions in plants. Renilla luciferase under the control of a CMV ...white screening can be used for screening of transformants in cloning process. Lynne Postovit 64034 pGL4Luc-RLuc...fragment for split-luciferase complementation assay in plants using Gateway cloning. Use with 174051 Alberto ...fragment for split-luciferase complementation assay in plants using Gateway cloning. Use with 174050 Alberto ...Transcriptional unit for firefly luciferase expression in plants (GoldenBraid 2.0). Diego Orzaez 68215 pEGB 35S:...

...and their research. These researchers span four continents and research diverse topics including microbiology... University of Ibadan. She works with medicinal plants such as Launaea taraxacifolia (Willd), an annual...renewable sensor, a bacterium that can sense contaminants and emit light as a response. To create this...fluorescent protein transcription in response to river contaminants and transform these plasmids into a bacterial...geographical locations. In addition to providing reagents and educational resources for scientists globally... Check out our CRISPR resources and CRISPR for plants Bacterial Expression Systems Synthetic Biology ...

... also shorter than those needed for Cas9 (42 nt vs 100 nt.) However, the newly described non-CRISPR protein...Additionally, whereas Cas9 generates blunt ends 3 nt upstream of the PAM site, Cpf1 cleaves in a staggered...smaller than any of these options (2.7 kb with 24 nt guides), so it has the potential to eclipse any of...into AAV when you add in a gRNA and regulatory elements. To solve this problem, the Zhang lab has previously...Kleinstiver et al., 2016. Until recently, two major elements of Cpf1 characterization were missing: on-target...

...bimolecular fluorescence complementation experiments in plants. The Zinc Finger Consortium Modular Assembly...plasmids that can help researchers plan their experiments using bacterial expression systems. Read below...proteins of interest to transcriptional regulatory elements that will turn on gene expression upon protein–protein...generation of FRET biosensors for ratiometric measurements. The MoBiFc Toolkit (Addgene #1000000222) is...Plasmids Reporter plasmids can be used to detect events and molecules in and around a bacterium. Plasmids...Chromoproteins: Colorful Proteins For Molecular Biology Experiments Return to top Genome Engineering Modifying the...cellular resources otherwise spent on producing high amounts of protein. Whether you're investigating basic ...

...C-terminal fragments and placed under the control of different promoters. Expression of both fragments in the... gene expression, and find plasmids for your experiments. Plasmid...Site Canonical Sequence Structure (Length) Common Variants Cre loxP ATAACTTCGTATAgcatacatTATACGAAGTTAT 13...system allows scientists to utilize recombination elements such as Cre to turn off the expression of one ...combined in multiple, independent recombination events in a single experimental system, enabling multi-layered...lineage-specific drivers can also help reduce off-target events and the systemic effects of Cre toxicity. Leakiness...iCre variant, reduces the number of recombination events but can improve specificity in targeted neuronal...

...editing in the yeast P. pastoris (a.k.a. K. phaffii ). NT-CRISPR Plasmid Collection Genome Engineering, CRISPR...fluorescence complementation experiments to analyze protein-protein interactions in plants. MoClo-YTK Yeast Expression...from different combinations of a common set of components (Weber et al., 2011). This method, based on Golden...cut outside their recognition site, leaving DNA fragments with an overhang of 4 bp. If each DNA module is...assembly steps. Before beginning, scientists insert fragments of DNA containing basic parts (promoters, UTRs...transformation. Figure 1: Overview of the MoClo Toolkit components and assembly. Image from Weber et al., 2011. ...Voytas 93 additional plasmids including regulatory elements, coding sequences, CRISPR parts GreenGate Cloning...

...for many years and lead to a major burden on the patients, their families and caretakers, and the health...general. The required care for these millions of patients results in billions of dollars spent each year...changes in behavior and emotion, uncontrolled movements, memory deficits, as well as difficulty speaking...after a person's mid-sixties. The early-onset form accounts for less than 10 percent of all cases of Alzheimer's...plasmids expressing wild type or mutant ORFs, gene fragments, and gRNAs for genes linked to neurodegenerative...et al. Nat Commun. 2022) Endogenously tag gene variants for Alzheimer's disease and related dementias ...discovery and advance development of diagnostics and treatments for Alzheimer’s disease and related disorders...

...tetracycline-regulated gene expression and find plasmids for your experiments using tet- or dox-inducible systems....system has been engineered with a variety of improvements. A synthetic Tet Response Element (TRE) typically...been designed by combining tet O sequences with elements like the H1 RNA promoter. And, most importantly...Tet-Off and Tet-On. Read on to learn more about the components of tet systems and how to use them, or skip ahead...activation domain). For simplicity, other promoter elements and transcription machinery are not shown. Tetracycline... Systems Subsequent efforts have improved the components of these systems for better efficiency, sensitivity...). rtTA3 and Tet-On 3G : further improved rtTA variants with lower background activity and greater sensitivity...

...to be diverse in topology, size, and isoelectric points. Pooled Libraries Pooled Library name Type PI Description...payment, and billing. Industry Material Transfer Agreements Most Addgene orders must be accompanied by a ...protection for material providers. Addgene's deposit agreements allow Addgene to distribute plasmids on behalf...behalf of the depositing institutions. These agreements indicate the language which we are allowed to use...use for distribution. Please see the Sample Agreements on our Technology Transfer Information page for ...authorized signatory is someone who can execute legal documents on behalf of the recipient institution. Once the...addgene.org . After we review and approve the MTA documents, your order will be processed and shipped. Industry...

...Empty backbones are plasmids containing only the elements needed for replication in the host cell and are...choosing what plasmid backbone to use, you have many elements to consider. Here is a guide to a selection of... 3x HA tag fusion with your gene of interest in plants Myc Epitope tag pKMyc - N-terminal Myc tag for ...-nosT - Transient expression vector for eGFP in plants Find FP tagging vectors in budding yeast pHT101... transient expression is sufficient for some experiments, scientists often want to create stable ...species Vectors for RNA-guided genome editing in plants Visit our CRISPR Plasmids and Resources collection... miRNA and 3' UTR mRNA regulation pIS0 - 3'UTR segments of target genes can be inserted into this firefly...

...The RESOLUTE mission is to develop data and reagents to boost the research focused on Solute carrier ...Union. The RESOLUTE mission is to develop data and reagents to boost the research focused on SLCs. Through...-access ethos’ to the results, techniques, and reagents with the highest-possible quality of research ...knowledgebase that features, among others, genetic variants and disease associations. Plasmids This plasmid...libraries are useful tools for genetic screening experiments. These libraries target the solute carrier family...new window) . If you have further questions or comments you can also contact them at [email protected]...

... collections and resources are up-to-date with reagents and information you need to use CRISPR in your... for a variety of organisms, including mammals, plants, bacteria, yeast, Drosophila, zebrafish, C. elegans...of plasmids that are used together in CRISPR experiments. CRISPR Pooled Libraries : Find CRISPR libraries...knockout, activation, inhibition, and barcoding experiments. Browse CRISPR Plasmids by Function Genome Engineering...deletions. Insert CRISPR transposases insert large fragments of DNA. Transcriptional Regulation Activate dCas9...parasites. Plant CRISPR plasmids designed for use in plants. Yeast CRISPR plasmids designed for use in yeast...

...Purchase and the applicable material transfer agreements (MTAs) and limited use label licenses (LULLs)...10 business days in order to proceed with any adjustments to the original order. Turnaround Time Production...10 business days in order to proceed with any adjustments to the original order. Experimental Disclaimer...Disclaimer Due to the novel nature of experiments carried out with our materials, Addgene cannot guarantee the...assists importation by including thorough shipping documents and following import procedure, it is the responsibility...

...pseudovirus, where the structural and functional components of one virus (such as HIV, murine leukemia virus...information. Plasmids Expressing Spike and Spike Variants Full-length wildtype SARS-CoV-2 spike protein ...D614G change which is present in many circulating variants. The table below lists expression plasmids that...Other Resources Protocols Bloom lab - Protocol and Reagents for Pseudotyping Lentiviral Particles with SARS-CoV...Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants Addgene COVID-19 Resources and Collections COVID...

...(dark) acceptor, used for FLIM measurements Note that FRET measurements can be influenced by many factors...Find plasmids and resources for FRET experiments to measure protein interactions or conformation changes...molecules have been demonstrated for use in FRET experiments. FRET efficiency can be measured using fluorescence...Introduction to FRET and Tips for Using FRET in Your Experiments . Find many FRET-based biosensors in Addgene'...

...Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants Return to top Other Coronavirus Plasmids: SARS-CoV...Whitehead Libraries of Spike (S) Ectodomain and RBD mutants. SARS-CoV-2 Spike Receptor Binding Domain Deep ...Extraction Without A Kit Bloom lab - Protocol and Reagents for Pseudotyping Lentiviral Particles with SARS-CoV...Link opens in a new window) COVID-19 SARS-CoV-2 preprints from medRxiv and bioRxiv (Link opens in a new ...differentially and only proteolysis by TMPRSS2 augments entry driven by the severe acute respiratory syndrome...

...generates “clean” homozygous mutants with no co-integrated markers or footprints, and can be scaled up for...This CRISPR/Cas plasmid-system consists of two components: A vector containing Cas9 and a validated gRNA...More details on how to use this plasmid in your experiments can be found below and in the supplemental materials... as homology arms. The system consists of two components: An all-in-one CRISPR/Cas9 vector based on the...

... launched in 2008 to drive the development of treatments and cures for Rett Syndrome and related MECP2...syndrome is a neurodevelopmental disorder that presents in early toddlerhood primarily affecting females...skills, notably loss of acquired purposeful hand movements and regression of speech. In contrast to the sustained...there is wide variability in symptoms even within patients with the same mutation, the location and type ...2007. Correlation between clinical severity in patients with Rett syndrome with a p.R168X or p.T158M MECP2...