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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 304 results for: pET

Showing: 41 - 60 of 304 results

  1. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    ... Figure 1: Schematic of native (nuclease-competent) Cas9 interacting with either a full-length gRNA...relies on two distinct Cas9 variants—nuclease-competent or nuclease-null—in order to effect different ... to Cas9. For instance, when native (nuclease-competent) Cas9 interacts with a ≤15nt gRNA that contains... Figure 2: Gene activation with nuclease-competent Cas9. An RNA hairpin incorporated into the truncated...  Figure 3: Gene repression with nuclease-competent Cas9. When complexed with a truncated gRNA, Cas9...Cas9 is still able to bind DNA but is no longer competent to cut DNA. When directed to a site downstream...delivered into systems that already express nuclease-competent Cas9, obviating the need to generate new cell ...

  2. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    ...genetic tools. Mobile-CRISPRi from the labs of Jason Peters, Oren Rosenberg, and Carol Gross provides a streamlined...Mobile-CRISPRi's modular components. Image from Peters et al., 2019 with permission.   Two methods... with an integrated copy of CRISPRi. Image from Peters et al., 2019 with permission.    The team showed...strains and environmental isolates. For example, Peters and colleagues used Mobile-CRISPRi to knockdown...possible with Mobile-CRISPRi. As a proof of principle, Peters and colleagues constructed a 40-member library ...microbiology 4.2 (2019): 212. PubMed PMID: 30675034. Peters, Jason M., et al. "Enabling genetic analysis of...PMID: 30617347. PubMed Central PMCID: PMC6424567. Peters, Joseph E. "Tn7." Mobile DNA III. American Society...

  3. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

    Type
    Blog Post
    ...all bacteria are naturally competent to take up DNA, they can be made competent through chemical manipulation...Addgene’s blog to learn about making your own competent cells and our protocols page to learn about bacterial... The phagemids are packaged into replication-incompetent phage particles with assistance from a ‘helper... A., and Karen Guillemin. "Multiple phases of competence occur during the Helicobacter pylori growth cycle...Meibom, Karin L., et al. "Chitin induces natural competence in Vibrio cholerae." Science 310.5755 (2005):...

  4. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

    Type
    Blog Post
    ... and tested in the “market” by competing in business plan competitions. After much learning, the focus...plan competition and advance investors to the next stage of diligence." In business competitions everything...concept development and participating in business competitions. When the team was unable to secure enough money...CV tips - LabSpaces Blog Find a Buisness Plan Competition Good Relations - PR for the Biotech Business ...

  5. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    ...200 ul pipettor is set to 200 ul, wiped down with 70% ethanol, and a box of sterile truncated pipet tips...the pipettor, placed into wells, and partially dislodged from the agarose plug by rapid pipetting, as ...rapidly punch out cell clones with sterile truncated pipet tips. Indeed, an experienced operator can pick 48.... We highly recommend the use of an electronic pipettor with variable spacing (such as the Viaflo from...

  6. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...Anti-Keyhole limpet hemocyanin [N132A/12R] Keyhole limpet hemocyanin Giant keyhole limpet Mouse IgG2a ...

  7. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    ...Prior to March 2020, internships were a fiercely competitive endeavor. Due to the COVID-19 pandemic, students...college, and graduate students. In order to gain a competitive advantage this cycle, consider the advice below...counterintuitive since that approach seems to create competition for your own spot. True! But you create a network...sense approaches that will prepare you for the competitive landscape beyond college. They will also serve...graduation. While these suggestions increase your competitive edge, they alone, are no guarantee of success...

  8. Pooled Library Amplifications

    Type
    Blog Post
    ...plasmids, they are generally electroporated into electrocompetent cells to ensure the highest possible transformation...use for your transformation(s). The amount of competent cells you need total and per transformation may...liquid culture, all of the bacteria can grow and compete in the same container, making it easier for a smaller...be its own colony, and will not be in direct competition with other colonies containing smaller, recombined...recommend ensuring the cells used are meant for repetitive sequences (such as STBL4 or Endura Duo), growing...

  10. Great Results Start with Great Standard Curves

    Type
    Blog Post
    ...curve, avoid pipetting small volumes (< 2 µL) or volumes too large for a standard micropipette (> 1,000 µL...2. When preparing a dilution series, use a new pipette tip for each step and mix the samples well by vortexing...readout. When preparing a standard curve, change pipette tips and mix thoroughly by inversion or vortexing...series, use consistent dilution factors, change pipette tips regularly, and mix samples very well.  Use...

  11. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    ...the same replicon are incompatible because they compete for the same replication control machinery within... concept is a little more complex than simply competing for replication control factors and tends to be...plasmids with the same centromere-like binding region compete for the same CBPs and NTPase to correctly partition...blocking replication of another and one plasmid outcompeting the other plasmid for replication or partitioning...machinery (Novick, 1987). In cases where one plasmid outcompetes the other, usually a smaller, higher copy plasmid...

  12. QC Sequencing Technologies at Addgene

    Type
    Blog Post
    ...Sanger sequencing to confirm an insert that is repetitive or otherwise hard to sequence using NGS or long-read...such as G-C rich areas, secondary structures, or repetitive regions. Short-read sequencing can also be time-consuming...sequencing or Sanger sequencing, either because of repetitive regions or for higher accuracy of a particular...This method is great for resolving complex and repetitive sequences, but can be costly, low-throughput,...Nanopore sequencing to QC plasmids that are highly repetitive, such as those containing cassettes of the same...

  13. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    ...want to use electro-competent cells instead of the more common chemically-competent cells. You should perform...Follow the manufacturer’s instructions for your competent cells. For most standard cloning, you can transform...transform 1-2μl of your ligation reaction into competent cells such as DH5alpha or TOP10. If using much ...getting colonies, you might want to use higher competency cells. Additionally, if your final product is...

  14. Viral Vector Guides and Plasmids

    Type
    Collection
    ...carefully engineered for safety and are replication-incompetent. Specific genes have been mutated or deleted ...

  16. 2018 #DeckTheLab Contest Winners

    Type
    Blog Post
    ... appreciated the creative uses of old posters, pipette tip boxes, and more (stay tuned for a few blog ...sustainability in the lab!). The @ladiesinlabcoats used a pipette tip box and microcentrifuge tubes to make Eppi ...labchristmas #cute #reindeer #DeckTheLab #eppendorf #themuppets #scifun #funny #ChristmasScene #womenwhoscience...all who participated in the 2018 #DeckTheLab competition. The four winners will receive a holiday care...

  17. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    ...forming a vector that could create replication competent virus. Even though this system was efficient at...potential risk for the generation of replication competent lentiviruses (RCL) especially if you are working...positive cells could make the virus replication competent. The 3rd generation of lentiviral vectors was...8 kb) Potential for generation of replication-competent lentivirus (RCL) Efficient gene transfer Potential...Potential for insertional mutagenesis: Replication-incompetent lentiviruses with human tropism can still infect...

  18. From our Table to Yours: An Inside Look at Lunch at Addgene

    Type
    Blog Post
    ...lab. Iron Chef-style competitions Every few months, we’ll have a cooking competition featuring a selected..., which is either an indication of the stiff competition or says something about my cooking.   This is... to organize. We’ll dedicate a post to these competitions in the future, and hopefully include some of...

  19. Promega Plasmid Collection

    Type
    Collection
    ...apparent affinity of test compounds is measured by competitive displacement of a NanoBRET tracer, reversibly...

  20. Extracurricular Activities for a Strong Science Career Path

    Type
    Blog Post
    ...great approach to fully understand and acquire competitive skill sets in a myriad of professions is to engage...Mentorship Leadership Science writing competitions (e.g. Access to Understanding)   Writing...Communication Networking Publicity Business competitions   Leadership Management Marketing ...opportunity to tailor their career paths for competitive positions both within and outside academia. Use...
Showing: 41 - 60 of 304 results