We narrowed to 63 results for: pcas9

... for reduced off target editing, such as SpCas9-HF1, eSpCas9(1.1), or HypaCas9. Read more about Cas9 options...

... deaminase portion of the base editor to create SpCas9 base editors with editing windows as small as 1...available fused to ABEmax(7.10) or to BE4max   Evolved SpCas9 with NRN or NYN PAM site Chen et al., 2023 Td-CBE...

...compared to the most commonly used Cas9 variant, SpCas9. These properties, combined with other benefits...

...create a colorful Cas9 toolbox, Ma et al. turned to SpCas9 and its orthologs NmCas9 and St1Cas9. Each ortholog...

...editing in moderate thermophiles.  These evolved SpCas9 variants have improved activity on NAG PAMS and...

...nickase experiment Overview of Cas9 nickase We’ll use SpCas9 nickases as examples for this post. The D10A mutation...

...The PAM Requirement and Expanding CRISPR Beyond SpCas9 The closer your edit is to the PAM site, the higher...

...887 amino acids in length, NgAgo is shorter than SpCas9 (1368 aa), SaCas9 (1053 aa), or Cpf1 (1228 or 1307...

...different fluorescent protein to various Cas9 variants, SpCas9, NmCas9, and St1Cas9. These fusions localized to...

...constructs using a high efficiency intron-optimized SpCas9-coding gene in plants. CRISPR Sylvestre Marillonnet...

...with these modified gRNAs is comparable to that of SpCas9. The group also confirmed that the Cas12f1 and ...

...create a colorful Cas9 toolbox, Ma et al. turned to SpCas9 and its orthologs NmCas9 and St1Cas9. Each ortholog...

...constructs. zCas9i is a high efficiency intron-optimized SpCas9-coding gene. SlugCas9 is a compact Cas9 ortholog...

...is more stable in human plasma than the standard spCas9 (Harrington et al. 2017). Lastly, as with any scientific...

...regions and their reverse complements were parsed for spCas9 PAM sites (NGG) and then filtered based on two ...

...-ko-sgRNA1-SpCas9 TARDBP U6 ALS Rajat Rohatgi 107858 pHBS953 TARDBP-Exon2-ko-sgRNA2-SpCas9 TARDBP U6 ALS...pEGFP-C9orf72 C9orf72 GFP CMV ALS Shawn Ferguson 83439 pSpCas9(BB)-2A-Puro-C9orf72 C9orf72 U6 ALS Shawn Ferguson...Parkinsonism, early-onset Alessandro Ori 221550 pSpCas9-2A-Puro-TBK1-gRNA (PX459) TBK1 U6 ALS Shawn Ferguson...

...also fused LSD1 to x-Cas9(3.7). x-Cas9(3.7) is a SpCas9 mutant that exhibits increased PAM flexibility,...

...There are three subtypes, A–C, with the beloved SpCas9 (from Streptococcus pyogenes) belonging in II-A...

...technique was applied to create photoswitchable spCas9, dCas9, and S. aureus Cas9 (saCas9) proteins, showing...

... From the lab of Yonglong Chen, pCS2-3xFLAG-NLS-SpCas9-NLS is a Cas9 expression plasmid that was used ...