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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 67 results for: pcas9

Showing: 41 - 60 of 67 results

  1. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ... deaminase portion of the base editor to create SpCas9 base editors with editing windows as small as 1...available fused to ABEmax(7.10) or to BE4max   Evolved SpCas9 with NRN or NYN PAM site Chen et al., 2023 Td-CBE...

  2. Lentiviral Prep Service

    Type
    Collection
    ...-targeting controls. This backbone contains SpCas9. SpCas9 and 76,441 unique sgRNAs targeting 19,114 human...non-targeting controls. This backbone does not contain SpCas9. 76,441 unique sgRNAs targeting 19,114 human genes...non-targeting controls. This backbone does not contain SpCas9. 78,637 unique sgRNAs targeting 19,674 mouse genes...

  3. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...Cas9s: Three high fidelity Cas9 variants , SpCas9-HF, eSpCas9, and HypaCas9 display very low off-target...Researchers have expanded the CRISPR field beyond SpCas9, moving us closer to being able to target every... Staphylococcus aureus (SaCas9) is smaller than SpCas9 and more easily packaged in AAV. Cas9 orthologs...

  4. CRISPR Plasmids - Tagging

    Type
    Collection
    ... AAVS1 genomic safe harbor locus using untagged SpCas9 (Addgene 41815 or #44719 ) in combination with ...using an all in one vector from the Doyon lab, eSpCas9(1.1)_No_FLAG_AAVS1_T2 (Addgene #79888) , which ... integrating at the AAVS1 "safe harbor" locus: eSpCas9(1.1)_No_FLAG_AAVS1_T2 Doyon Lab TAP Tagging Protocol...

  5. Editor's Choice, July 2016

    Type
    Blog Post
    ...compared to the most commonly used Cas9 variant, SpCas9. These properties, combined with other benefits...

  17. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...purposes. NOTE: Here we discuss the usage of the pSpCas9(BB) plasmid (pX330) (Addgene plasmid ID 42230)....allows for the simultaneous expression of sgRNA and SpCas9, but does not contain markers for selection 1 ....

  19. Typing CRISPR Systems

    Type
    Blog Post
    ...There are three subtypes, A–C, with the beloved SpCas9 (from Streptococcus pyogenes) belonging in II-A...
Showing: 41 - 60 of 67 results