We narrowed to 204 results for: phen

...(GFP; Tzur et al., 2013), or rescue of mutant phenotypes (unc-119) (Dickinson et al., 2013). These approaches... for an editing event that produces a visible phenotype enriches for knock-outs and knock-ins at other... by isolating animals with particular visible phenotypes. These approaches may become even more powerful...

...use, there remains a chance that the observed phenotype was caused by an off-target mutation and not an...causal relationships between genotype and assayed phenotype. Verifying these connections can help alleviate...investigators looking to definitively link genotype and phenotype. It is also key to perform these validations as...

...development of tumors is a complicated and dynamic phenomenon that is interestingly responsible for tumor reduction...changes. Tumors differ in their genotypic and phenotypic identities based on their level of progression...A thorough understanding of the genotypic and phenotypic changes in the various types of cancer will fuel...

... that there are no positive controls for this phenotype – you’ve developed a new model or a new assay ...individual plasmids will be useful when screening for phenotypes, such as cell-non-autonomous effects, that do...

...pooled cell population, as mutations with strong phenotypic effects can mask weaker ones. Thus, the need ...possible that a given mutation will have a different phenotypic effect in a different system. These plasmids ...

...to increase the accessibility of CRISPR-based phenotypic screen datasets. Original published CRISPR screen...possible scope of screens to be curated, for example phenotype screens with read-outs other than cell viability...

...necessary to identify the gRNAs that elicit the phenotype, and this depends on the method used to introduce... the genome. Thus the gRNAs responsible for a phenotype can be identified by next-generation sequencing...

...identify and/or facilitate the evolution of new phenotypes, and forward genetics harnesses this at a large...genotypes that result in a spectinomycin resistant phenotype in bacteria. While there are many known spectinomycin...

...simply – they are systems that measure biological phenomena by generating an output which is directly proportionate...directly measure a cellular process. Cellular phenomena such as exocytosis, autophagy, voltage, and tension...

.... S. cerevisiae no  no TRP1 alters some yeast phenotypes. This marker should not be used in gene disruption...potential metabolic burden on the yeast cells. Some phenotypes may be altered due to the presence of the selection...

... Optional: Perform phenol-chloroform extraction - see protocol below . Note: Phenol-chloroform extraction...When phenol is mixed with the aqueous solution containing DNA, proteins will move into the phenol phase...Protocol: Phenol-Chloroform Extraction of DNA Samples Add an equal volume of TE-saturated phenol-chloroform... solution 2 mg/mL RNase A TE or water-saturated phenol-chloroform Chloroform 100% ethanol or isopropanol...the aqueous DNA sample. Pro-Tip Water-saturated phenol-chloroform can be used if TE-saturated is not available...recovered aqueous DNA layer. Repeat steps 2-4. Note: Phenol-chloroform is a hazardous waste - DO NOT pour down...purified without a kit it is advisable to perform a phenol/chloroform extraction of the supernatant after ...

... Optional: Perform phenol-chloroform extraction - see protocol below . Note: Phenol-chloroform extraction...When phenol is mixed with the aqueous solution containing DNA, proteins will move into the phenol phase...Protocol: Phenol-Chloroform Extraction of DNA Samples Add an equal volume of TE-saturated phenol-chloroform... solution 2 mg/mL RNase A TE or water-saturated phenol-chloroform Chloroform 100% ethanol or isopropanol...the aqueous DNA sample. Pro-Tip Water-saturated phenol-chloroform can be used if TE-saturated is not available...recovered aqueous DNA layer. Repeat steps 2-4. Note: Phenol-chloroform is a hazardous waste - DO NOT pour down...purified without a kit it is advisable to perform a phenol/chloroform extraction of the supernatant after ...

...interest in the cancer microenvironment Ever since Stephen Paget’s “seed and soil” theory in 1889, microenvironments...step closer to understanding cancer as a broad phenomenon and not only as an isolated disease. While the...

... networks underlying your favorite biological phenotype in a scalable way. This blog post will introduce...combinatorially modify the genome, screen for a particular phenotype, and quickly profile the resultant hits (Cheng...

...barcode. This collection can be used for in vivo phenotypic screening of small-molecule libraries and can...reveal how genetic alterations lead to changes in phenotype. The plasmid comes with a 1.9 kb stuffer sequence...

...These diseases are characterized by overgrowth phenotypes and neural developmental delay. Mutations in ...in loss of function or dominant negative NFIX phenotypes. Developing a rapid test for the functional ...

...the presence of the plasmid corrects any known phenotypic abnormalities due to the deletion (i.e. the plasmid... plasmid complements the deletion). If the phenotype is corrected, students can be relatively confident...

...zygotes, five had detectable Tyr editing at the phenotypic and genotypic levels. The editing efficiencies... injected with Nanoblades targeting the hydroxyphenylpyruvate dioxygenase (HPD) gene, all nine treated...

...decreases. Crossing mice to generate a desired phenotype Instead, genetic engineering should be used to... this series). In any case, Cre is a dominant phenotype, while you’ll want the floxed gene to be homozygous...

...form of FACS-based cell screening, Single-cell Phenotypic Observation and Tagging with Light (SPOTlight...isolate individual cells with unique spatiotemporal phenotypes from large heterogeneous cultures.  HeLa...