We narrowed to 183 results for: tTA

... protein. These RNA molecules are vulnerable to attack by both cytoplasmic and nuclear nucleases, as they...group of the ribose to protect it from nucleolytic attack. Another stabilizing sugar modification, found ...

...separate them. Most proteins are not likely to remain attached to the DNA throughout the sample processing and...sample may need more or less cross-linking to firmly attach the proteins to the DNA, depending on how tightly...

... used to turn “on” and “off” MEK1 activity by attaching two dimerizing photoswitchable Dronpa (dron= a...at sites homologous to the locations they were attached to in MEK. The photoswitchable versions of MEK1...

...method relies on multiple secondary antibodies attaching to a single primary antibody, it is sometimes ...reality, multiple primary antibodies can and do attach to a single target protein, so this assumption ...

...genes that help bacteria invade hosts, including attaching to host cells and evading the host immune system...resistance plasmids, which protect the host from attacks by other organisms. To conserve energy, these specialized...

...Despite these grim statistics, new strategies for attacking cancer are yielding promising results in the laboratory...universally-dividing behavior of cancer cells, attacking the cellular machinery necessary for division....

...geothermalis (Dge10TnpB) targets a different TAM (5′-TTAT-3′). The unique TAM requirements expand the number...5: Colony Screening Use Primer 92F (5′-cattacgcaattggacgacaac-3′) and Oligo 2 (specific to your target...

..., 481–485. PMID: 26098369 Kleinstiver, B. P., Pattanayak, V., Prew, M. S., Tsai, S. Q., Nguyen, N. T.,...PMID: 25398340 Walton, R. T., Christie, K. A., Whittaker, M. N., & Kleinstiver, B. P. (2020). Unconstrained...Daringer, N. M., Freije, C. A., Myhrvold, C., Bhattacharyya, R. P., Livny, J., Regev, A., Koonin, E. V.,... Naito, Y., Nakada, S., Yamamoto, T., Sano, S., Hotta, A., Takeda, J., & Mashimo, T. (2019). CRISPR-Cas3...

...constructs: pLVX-UbC-rtTA-Ngn2:2A:EGFP (Schafer et al. Nat Neurosci. 2019) pLVX-UbC-rtTA-Ngn2:2A:Ascl1 (Herdy...

...covalently linked molecules (i.e. photoswitches) attached near the binding pocket of an engineered receptor...PORTLs use a similar concept, except the ligand is attached to a protein binding partner or nanobody that ...

...are small plastic containers that can easily be attached with a hose to a nitrogen gas tank with a pressure...encoded by the plasmids in the library will remain attached to the membrane at the same location that their...

...What’s my angle on attacking this problem?”, try thinking “Who can I assemble to attack this problem from...

... at the terminal 3’ PAM site (Fu et al. 2013; Pattanayak et al. 2013). This approach misses larger mismatches...25595557. PubMed Central PMCID: PMC4308725. 7. Pattanayak, Vikram, et al. "High-throughput profiling of...

... analysis, you'll need to use fluorophores that attach to the target. When you design a flow experiment...cells with fluorophore-conjugated antibodies that attach to a specific target. By using a mix of different...

... before.  These attacks on science ultimately galvanized scientists to rally in Ottawa to protest the ...

...the nascent heteroduplex and abolish cleavage (Pattanayak et al., 2013). As mentioned above, however, Cas9...e96941. https://doi.org/10.15252/embj.201796941 Pattanayak, V., Lin, S., Guilinger, J. P., Ma, E., Doudna...

... outcome, since not all cell types are easily attainable, or easy to reprogram. Basically, there’s no ...Deepak M. Gupta, Mei Huang, Zongjin Li, Nicholas J. Panetta, Zhi Ying Chen, Robert C. Robbins, Mark A. Kay,...

...telomeres and found that TZAP binds directly to TTAGGG repeats via its zinc finger domains. This binding...binding was also independent of another TTAGGG binding complex, shelterin. Swapping the DNA binding domain...

...cells stably expressing pTRE-Tight-MitoTimer and rtTA. The cells were pulsed with 2µg/ml of Doxycycline...expression and purification of Cas9 protein in Rosetta E. coli. New Lentiviral CRISPR activator and repressor...

...Matsuzaki, Y., Konno, A., Mochizuki, R., Shinohara, Y., Nitta, K., Okada, Y., & Hirai, H. (2018). Intravenous ...Vormstein-Schneider, D., Lin, J. D., Pelkey, K. A., Chittajallu, R., Guo, B., Arias-Garcia, M. A., Allaway, K...