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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 265 results for: ttl

Showing: 41 - 60 of 265 results

  2. Plasmids 101: The protein expression toolbox

    Type
    Blog Post
    ...vector and Murine LoxP vector.   Turn it off for a little while Transient suppression of expression is faster... Lenti expression vector. Turn it on for just a little while Yes, there’s a CRISPR for that, too! CRISPR...

  6. How to Prepare for an Industry Interview

    Type
    Blog Post
    ...excited for this next step. But wait, now you feel a little panicked. You’ve never had an industry interview...day.  This whole process may seem foreign and a little overwhelming, but here is what I’ve found helpful...

  7. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    ...The PAM… that sneaky little bit of sequence that you hope is present next to the "perfect" guide sequence...make a precise amino acid change, there is very little flexibility in the location of the cut and subsequent...

  8. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    ... in 1947 in Uganda, the Zika virus had received little attention and, for the most part, had been associated...databases to the production of validated sensors in as little as one week (Pardee et al, 2016). Using toehold...

  10. Plasmids 101: Degron Tags

    Type
    Blog Post
    ...the protease cleavage sequence. The tag is then shuttled to the proteasome due to the presence of the degron...tagged protein. In this event, the degron domain shuttles the entire tagged protein to the proteasome for...

  11. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    ..., creating a presentation for 8th graders was a little intimidating. Too simplistic and I would insult...section was arguably the most important, and it was a little worrisome for me since bioethics is an inherently...

  12. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    ...feeling optimistic for the next tournament). Minus a little rain towards the end, it was another successful.... I’m aware that  “organizing the fun” sounds a little neurotic, but hey- sometimes fun needs to be had...

  14. Lab to Office Culture Shock

    Type
    Blog Post
    ...Leaving a tight-knit team like that can and will be a little gut-wrenching, but you can get that feeling of ...editor, etc.--so everyone’s experience will be a little different. Some of these changes you may enjoy ...

  15. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ...access to reagents and equipment has become a bottleneck. In the last few years, scientists have utilized... work rapidly, providing reliable results in as little as an hour. Find the SHERLOCK plasmids at Addgene...

  16. How to Be an Excellent Trainee

    Type
    Blog Post
    ...accordingly in case you have questions or need a little assistance at some point during the procedure. ...with questions and concerns is invaluable. With a little thought you can build meaningful relationships ...

  17. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    ...should be able to find your mutant from screening as little as 8-10 pups. The level of fluorescence you saw...day you have your F0 generation (barring life’s little accidents and stuff ups). This is a huge improvement...

  18. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    ...people until the day of the AMA. This made it a little difficult to promote the AMA on social media beforehand...relationships. I also like Twitter because I can get a little opinionated and, while it can be anonymous, I can...

  20. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ...entities on our planet, but we still know collectively little about how they infect and influence the evolution...be choc-full of open reading frames that display little to no homology to anything else, making it difficult...
Showing: 41 - 60 of 265 results