We narrowed to 625 results for: Emb

1. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

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   Blog Post

   ...al. 2016. “Inhibitory Synapses Are Repeatedly Assembled and Removed at Persistent Sites In Vivo.” Neuron...

2. Healthcare Consulting: A Door to the Business of Life Sciences

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   Blog Post

   ... on the expertise of the senior leadership and members of their consulting staff. Who would enjoy healthcare...

3. Getting the Most from Your Lentiviral Transduction

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   ...., Rubinstein, M. “LDL receptor and its family members serve as the cellular receptors for vesicular stomatitis...

4. Plasmids 101: Control Plasmids

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   ...difference and is it significant? Vaux DL, et al. EMBO Rep. Apr; 13(4): 291–296. (2012). PubMed Central...

5. Tips for Using FRET in Your Experiments

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   ...Applications: An EAMNET Teaching Module http://www.embl.de/eamnet/downloads/modules/FRET_teaching_module.pdf...

6. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

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   ...luck with any experiments involving IDLVs. And remember: You can never trust a lentivirus with secrets...

7. Antibodies 101: Introduction to Gating in Flow Cytometry

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   ...running your analysis. In this case, I and other lab members had worked with lymphocytes before, so I knew where...

8. Harnessing Bacterial Toxins for Allelic Exchange

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   ...excellent efficiency using a commercial isothermal assembly master mix. Introduction of the strong transcriptional...

9. Transferable Skills Guide: Time Management

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   Blog Post

   ...physically cross things out (it just feels good) and I remember things better when I write them down. Some people...

10. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

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    ...deliver these components to your target cells. Remember, efficient delivery of CRISPR components is essential...

11. Evolution of Lab Techniques

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    ...smaller pieces, run on a gel, and transferred to a membrane for analysis through hybridization of a radioactively...

12. Treating Muscular Dystrophy with CRISPR Gene Editing

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    ...Gene-editing summit supports some research in human embryos. Nature. 2015 Dec 3. doi:10.1038/nature.2015.18947...

13. Viral Vectors 101: AAV Variables That Matter

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    ...Attribution License. Within the nervous system, remember that some serotypes can be transported retrogradely...

14. Antibodies 101: Multiplex Immunofluorescence

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    ...this might be frozen or formalin-fixed, paraffin-embedded tissue sections, methanol- or paraformaldehyde-fixed...

15. Plasmids 101: Dimers and Multimers

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    ...control process. Since MiSeq involves sequence assembly from short reads (<300 bp) which cannot distinguish...

16. Recombinase-based State Machines Enable Order-dependent Logic in vivo

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    ...recommend using overlap extension PCR and Gibson assembly to piece together the registers. All parts used...

17. Building Global Connections with the International Mentorship Program USA-EUROPE

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    ...Director, Dr. Zafira Castano Corsino, a junior faculty member at Harvard Medical School/Brigham and Women’s Hospital...

18. Quick Guide to All Things Lentivirus

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    ...liposome) or electrical (electric current forming membrane pores) transfection. These techniques are still...

19. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

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    ...perfectly viable at 15 ºC, yet exhibit complete embryonic lethality at 25 ºC. This method resulted in efficiencies...

20. RNA Interference in Plant Biology: New Tools for an Old Favorite

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    ...undergoing RNAi, and LIIbeta F 1-2 RNAi, which allows assembly of intron-spliced hpRNAs through Golden Gate cloning...