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We narrowed to 1,104 results for: Ide

Showing: 871 - 900 of 1104 results

  1. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    ....  These days commercially available TOPO kits provide vectors or cloning arms with overhanging 3´ deoxythymidine... 2. Novel approach to molecular cloning and polynucleotide synthesis using vaccinia DNA topoisomerase....Molecular Biology Techniques Watch Our Protocol Videos ...

  2. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

    Type
    Blog Post
    ...methods for data analysis, our team’s goal is to provide scientists with new tools and protocols that they...labs looking to improve their AAV yields. Given the wide range of downstream applications scientists rely...resources of the Addgene blog Read our beginner's guide to viral vectors Find all of our viral vector blog...

  3. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    ...cellular-level events and behavior. It’s a problem that he considers “one of the greatest challenges of neuroscience...particular advantage for observing events taking place inside cells that are on the move, such as T lymphocytes...protein, troponin C (TnC), from muscle cells. They identified a toadfish TnC variant which had a high-affinity...

  5. DAP arrays for multiplex gene editing

    Type
    Blog Post
    ...CRISPR locus of a bacteria host encodes multiple guide RNAs (gRNAs) on a single array to target the genome...applications when repetitive tRNA sequences must be avoided, different tRNAs with optimized 5’ leader sequences...array for multiplex gene editing applications, we provide detailed manual and online design examples of using...

  6. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    ...coinjection into zygotes of Cas9 mRNA and a single-guide RNA targeting the mutant allele (7). An independent...CRISPR/Cas9 has been used to generate mutations in a wide range of organisms, but its potential to efficiently...These papers suggest it might work well enough to consider as a therapeutic.” There are many fundamental ...

  7. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    ...the pMinDisplay backbone is fused to a specific peptide segment (PDGFR TMD) that anchors the sensor to ...the membrane and displays it on the extracellular side. In addition to the original PDGFR TMD, the researchers...ordered directly from Addgene. The Podgorski Lab has provided a brief breakdown of the promoter systems you ...

  8. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    ...gene expression in a target cell. Practical considerations If you have high quality AAV, you can address... desired titer and want to dilute it, err on the side of caution when adding buffer back to the solution... for Work with Virus Watch Our AAV Purification Video ...

  9. Learning Technical Skills at a Lab Central Workshop

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    Blog Post
    ... 3 hours. It was robust, capable of generating a wide range of insert sizes and also produced high yields...interact and learn more about one another. They also provided both breakfast, lunch, and snacks. At the end ... to constantly update our processes and get new ideas from learning experiences like workshops at Lab ...

  10. Celebrate National Book Month with Addgene

    Type
    Blog Post
    ...center around a cast of endearing characters who decide to take matters into their own hands. Who doesn..., I wanted to be challenged to read some books outside of my comfort zone. I’d been in a bit of a reading.... We’ve read well-known classics, like Austen’s Pride and Prejudice, and some more obscure treasures, ...

  11. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    ...contrast” variant provides improved detection for tracking large populations of neurons in wide-field imaging...Addgene's viral service Find viral protocols and videos Find AAVs for your research ...

  13. Addgene Depositors Get More Citations

    Type
    Blog Post
    ... Science. For each publication, Web of Science provides basic bibliographic information and uses its vast...is very plausible that science published in more widely-read journals gets more citations and is more likely...citations due to the journal of publication. Table 1 provides an example calculation of the weights. Table 1...

  14. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    ...model yeast strain and discuss why you may want to consider a yeast off the beaten path.   Yeast as a model...disease models established in yeast, including a widely used Parkinson’s experimental model. In fact, approximately...of K. phaffii lacks certain terminal mannose glycopeptides (which most other yeast possess) that are problematic...

  15. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    ...used to infer differentiation trajectories and identify both up- and down-regulated pathways. Individual...unique barcodes for each TF help streamline identification. While the Zhang lab controlled their multiplicity... a model system. TF screens can be designed to identify custom TF programs to acutely initiate disease...

  16. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    ...that interact with the virus. As a result, we identified 332 human proteins that the virus interacts with...the help of human proteins in their lifecycle, identifying these potential human helpers of SARS-CoV-2 (...You could be the next scientist joining in this worldwide fight against SARS-CoV-2. As we’ve all been affected...

  17. Addgene Welcomes John Doench to the Advisory Board

    Type
    Blog Post
    ...bioscience and tech transfer communities who help guide and promote Addgene in its mission to make research...given by Phil Sharp. After a few rotations, Doench decided to complete his PhD work in Sharp’s lab.  Doench...a tremendous amount of technology development alongside it.” Doench starting working with David Root ...

  18. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    ...proteins to be imaged together, you will need to consider the same factors as when choosing an individual... the filter sets or lasers on your microscope. Ideally, you would like to have substantial overlap between...protein and a filter set, many filter set vendors provide tools to plot the fluorescence spectra of proteins...

  19. AAV Q&A with Tim Miles

    Type
    Blog Post
    ... kB, then you would be oversized even before considering the need for some manner of polyA. In this scenario...can be safely concentrated to ~1x1014 vg/ml.. As evident in our repository, this application is an outlier...Addgene.org Find Adenovirus Plasmids Adenovirus Guide Page Subscribe to Viral Vectors Blog Posts ...

  21. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

    Type
    Blog Post
    ...based on maps of protein structure. Sometimes this provides a great deal of clarity. But, often these methods...Institute Target Accelerator Plasmid Collections, now widely available to the scientific community through Addgene...Functional Interrogation of Rare Cancer Variants Identifies Oncogenic Alleles. Kim E, Ilic N, Shrestha Y....

  22. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    ...check out this blog post or see Addgene's CRISPR guide page for a review of Cas9.  For a brief comparison...Essletzbichler, P.,  Zhang, F. (2015). Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System. Cell...Research Catch up on Your CRISPR Background with Our Guide Pages ...

  23. Screening for Successful Genome Editing with Digital PCR

    Type
    Blog Post
    ...there are many great resources available to help guide you through the rough waters of mutation validation...great deal of precision. This means digital PCR provides an absolute measure of target abundance, does ...screening to validate your genome edits, this paper provides detailed instructions on how to design such assays...

  24. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    ...promoter should flank this component as well. On both sides of this promoter-gene combo are two long terminal... and have appropriate factors displayed on the outside of the particle to interact with the host. This...applications and aid in ease of use. Our lentiviral guide outlines these components.     Fig. 1 - ...

  25. Viral Vectors 101: Biosensors

    Type
    Blog Post
    ...within a biological system. Basically, biosensors provide quantifiable readouts for levels of different chemicals...FRET-based biosensors have been engineered to detect a wide array of neurotransmitters ranging from dopamine...fluorescence-based and have been optimized for a wide-range of uses to monitor the fundamental cellular...

  26. Plasmids 101: Methylation and Restriction Enzymes

    Type
    Blog Post
    ... and gotten unusual or unexpected results? Or considered why DpnI will degrade your template DNA from ...methylated (if it comes from a phage for instance). Aside from restriction modification systems, DNA methylation...level, the potential for methylation should be considered when digesting DNA. Why? Well, even though Dam...

  27. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    ...task and will prevent you from getting lost in the wide word of informatics. I am a molecular biologist ... project a few years ago when my advisor and I decided to do a massive RNA-seq analysis. I had never interacted...even if your conclusion is “this approach is not ideal because…” Including a conclusion for each analysis...

  28. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    ...through our hot plasmids articles. These articles provide brief summaries of recent plasmid deposits and ...can be reconstituted in vivo to form a protein identical in sequence to the unmodified enzyme. Each half...any exogenous sequences and regenerates a native peptide bond at the split site. Figure 1: The base...

  29. Working with Nuclear Receptors

    Type
    Blog Post
    ...interactome of these key regulatory molecules, and has provided clues to their previously uncharacterized cellular...acetyltransferase to luciferase, the basic assay remains widely used today.  The sheer abundance of these critical...greatly increased the burden associated with identifying plasmids in the research literature and acquiring...

  30. Rabies and Neuronal Tracing

    Type
    Blog Post
    ...molecular tools to map neuronal connectivity are widely used in neuroscience. In this post, I’ll describe... in first order neurons, the G-deleted RABV can divide and replicate (since these processes are mediated...Another study used RABV monosynaptic tracing to identify inputs to two major dopaminergic cell populations...
Showing: 871 - 900 of 1104 results