We narrowed to 1,052 results for: FER

... Even low-frequency editing could make a huge difference in DMD, and it’s estimated that exon skipping...respectively. Although each study used slightly different methods, they each observed the same positive ...disease type C. Although the three DMD studies referenced here represent a great step forward for CRISPR...many distinct CRISPR therapies to match these different mutations. For each therapy, effectiveness and...treatments may also be closer than we might expect.   References 1. Kaiser J. CRISPR helps heal mice with muscular...

...bottom of this post for a basic synopsis of the differences amongst the Brainbow systems. Pre-Brainbow techniques...to the promoter will be expressed. This system differs from Brainbow-1.0 in that transient Cre recombination...needed to mark individual neurons, the system also suffered from a number of limitations. First, the imaging...controlled by FLP/FRT recombinase. When placed under different promoters, Brainbow and Flpbow can be used to ...2.1 plasmids Brainbow 3.0, 3.1, 3.2 plasmids References: Transgenic strategies for combinatorial expression...

...PiggyBac transposons, the Woltjen Lab compared different variants of the polycistronic cassettes and discovered...a donor in FRET (fluorescence resonance energy transfer) and FLIM (Fluorescence Lifetime Imaging Microscopy...is a new member of this class that works via a different mechanism - the PhoCl protein is irreversibly ... are other RFP variants available that may be preferred for their intended uses, they provide a well-defined...of essential genes in vivo. Utilizing CRISPR interference (CRISPRi) technology, the arrayed knockdown ...defined domains can be swapped onto other proteins to confer functionality. In Narayan et al., they used this...

...Szymanski in January 2016 in Viruses (5). Please refer to the paper for more details on the assay, including...however, a physical fragmentation strategy is preferable. Nebulization involves forcing DNA through a ...on the membranes will then be exposed to a lysis buffer to release recombinant proteins directly onto the...then suspend a freshly harvested dose of cells (preferably in log phase) in blocking solution (around 108...thinking. Find her on Twitter @JessicaSacher.   References 1. Nobrega, Franklin L., et al. "Revisiting phage...

...Learn about different molecular cloning techniques, focusing on seven common cloning methods....Educational Resources Molecular Biology Reference Molecular Cloning Techniques Molecular Cloning Techniques...availability of starting materials, or just personal preference. Check out our blog on choosing the right cloning... entry , and Gateway destination vectors with different promoters, tags, selection markers, and fluorescent...

...“NCBI Site Preferences” box. Once on the Preferences page, navigate to “PubMed Preferences” and click ... that you know how to navigate to the PubMed Preferences and Filters pages, be sure to check out the other...

...Request service, which offers over 3,000 eligible transfer plasmids and five different serotypes. Not sure... pAAV_hSyn-PdCO-mScarlet-WPRE AAV1 Optogenetics Ofer Yizhar New viral tool pAAV-Ef1a-fDIO hChR2(H134R...

...modified allele is selectable, it can be transferred to a different genetic background via P1 transduction...Figure 2). In the following sections, key steps that differ from traditional restriction enzyme cloning will...could be used to move the modification into a different background, if needed. This approach is best suited...science communication and in vitro diagnostics.     References 1. Costantino N, Court DL. Enhanced levels of...

...AAV constructs CRISPR interference in Candida albicans A new CRISPR interference (CRIPSRi) platform for... to Cas9 SECURE base editors Nme2Cas9 CRISPR interference in Candida albicans E. coli genome-wide inhibition... Jennifer Tsang...

...animals’ skeletal muscles led to another clue: differences in the kynurenine pathway of tryptophan degradation... skeletal muscle expression of kynurenine aminotransferases, to enhance the conversion of kynurenine into...Get started with PGC-1 plasmids from Addgene. References: Skeletal muscle PGC-1α1 modulates kynurenine...M, Bhat M, Schuppe-Koistinen I, Pettersson AT, Ferreira DM, Krook A, Barres R, Zierath JR, Erhardt S, ...

...available at Addgene CRISPR-Cas9 References, Protocols, and more References: Generation of Gene-Modified ...the monkey story hit the presses, another very different bit of CRISPR news was circulating. Scientists... work. Last month, the Lawrence Berkeley Lab's Jennifer Doudna and Eva Nogales presented the first detailed...

... online. A few examples are provided here for reference: Untergasser Lab Preparation of chemically competent...use the Mix & Go! E. coli Transformation Kit and Buffer Set  from Zymo Research to make competent cells...then wash and resuspend the cells in the provided buffers. The competent cells are then aliquoted and stored...protocol)  Cells prepared using the Z-competent TM buffer can be easily transformed without a heat-shock ...

...finding the best way to organize your time. At a conference, you could be honing your science communications...that lab and interacting with the lab members.”- Jennifer Tsang, Science Communications and Marketing Coordinator...Addgene blog Read all careers blog posts Read our transferable skills guide Resources on Addgene.org Find... Jennifer Tsang...

...shipped over 350,000 individual plasmids to 5,000 different research institutions. This has given us a unique...by the influx of plasmids for reprogramming differentiated cells into pluripotent stem cells. Plasmids...HA-Ubiquitin-WT and HA-Ubiquitin, and we now have 19 different ubiquitin plasmids in the repository. 100,000 ... and modified by so many labs and for so many different purposes that we had to create a CRISPR page to...

...the researchers applied the LITE system to 28 different genes to establish it as “a novel mode of optogenetic...activators are easy to expand or combine with different effectors. I’d encourage people to get creative...apply LITEs for the transformation of neurons, she offers a tip: save yourself some time by making an AAV1...readers, how will you get creative with LITE? Reference: Silvana Konermann et al. "Optical control of ...

...York City, NY, USA 40.7128°N 74.0059°W – Deanna McLafferty I’m a New Yorker, so it’s not easy to convince...myths that abortions cause breast cancer and infertility or to argue against unnecessary clinic regulations...and fiction. Our health depends on it. Deanna McLafferty is an editorial assistant and activist. Washington...enough.  Nashville, TN, USA 36.1627°N 86.7816°W- Jennifer Stancill I am a research biologist by training...supporters is much larger and stronger that I expected. Jennifer Stancill, Ph.D., is currently a post-doctoral ...

...inflammatory cytokines - interleukin (IL)-12 and interferon gamma (IFNγ) - and eliminate cancer cells. 2....physiological and morphological changes. Tumors differ in their genotypic and phenotypic identities based...by cancer. As mentioned in this article, cancers differ in practically every facet starting from manifestation...manifestation to their specific interactions with different cell types. A thorough understanding of the genotypic...interested in exploring new avenues in healthcare.   References 1. Vinay, Dass S., et al. "Immune evasion in ...

...simplifies your viral vector options. Almost any AAV transfer plasmid in our repository can now be packaged ...packaged on demand in five distinct serotypes, offering you unparalleled flexibility. But this is just the beginning...developments? Subscribe to the Addgene newsletter. References Ayoubi, R., Ryan, J., Gonzalez Bolivar, S., Alende...A., Trimmer, J. S., Rego, M., Freedman, L. P., Ferrara, F., Bradbury, A. R. M., Cable, H., & Longworth...

... and Negative Selection for Plasmid Cloning by Jennifer Tsang There are many strategies for finding your...Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi by Jennifer Tsang Learn about mNeonGreen, mTFP1, and mWasabi...Precision and Flexibility to CRISPR Editing by Jennifer Tsang Prime editing is a new CRISPR technique... Jennifer Tsang...

... might not fold well, you should screen a few different vectors to see which gives you the most functional...two sets, each of which includes plasmids with different varieties of tags, both N- and C-terminal. Each...improve folding and disulfide bond formation. If one prefers expression in E. coli, the use of a thioredoxin...individually or as a complete kit from Addgene. References 1. Goulas, Theodoros, et al. "The pCri system...