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We narrowed to 957 results for: REP

Showing: 581 - 600 of 957 results

  1. Working 9-5: an Addgene Co-op

    Type
    Blog Post
    ...conduct a two hour long interview. I anxiously prepared for the call. The length of this interview was...was to do for my time at Addgene. This training prepared me for the independent work on the QC team. I ...and Geneious, I was able to determine if any discrepancies between what we sequenced in house and the submitted...

  2. A Better Way to Get Customer Support: The Help Center

    Type
    Blog Post
    ...is the 21st century and all. But while the auto-reply telling you your message was received is a nice ... on hold, let alone waiting multiple days for a reply to an email. You need answers: the right ones, quickly...everyone involved. We’re adding self service to the repertoire of tools you can use to get support for our resources...

  3. 6 Steps to Submitting a Resume That Gets Seen

    Type
    Blog Post
    ...for a successful application. It takes time to prepare an excellent resume…it is worth it. Read here to... to get help if you are having trouble preparing your most excellent resume. 2) Follow job boards and ...monitoring submissions (usually a Human Resources representative). Pro tip: if there is no place to upload a...

  4. SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture

    Type
    Blog Post
    ...SpyCatcher system, based on a protein isolated from Streptococcus pyogenes that locks itself together, to produce...they call SpyLigase (Spy comes from the “S” in Streptococcus and the “py” in pyogenes), capable of locking...links in the chain.  Addgene: What about the Streptococcus pyogenes protein domain made it ideal for this...

  5. Addgene at the ASM Microbe 2016 Conference

    Type
    Blog Post
    ...booth about amazing work already represented in the Addgene repository (like these new Rainbow Bacterial... we’ve curated microbiology plasmids from the repository onto one handy Microbiology Resource page and...

  6. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    ...CRISPR (clustered regularly interspaced palindromic repeats) Cas (CRISPR associated genes) system. This popular...coli in 1987 (1), the concept that these clustered repeat sequences operated as a safeguard against bacteriophages...for their ability to devise new strategies to reproduce within a host cell. A few virus particles have...

  7. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

    Type
    Blog Post
    ...primary antibody that is directly conjugated to your reporter circumvents the issue. However, directly conjugated...which uses just a primary antibody that has your reporter (in this case a fluorophore) directly attached...biorender.com. Proper tissue processing and sample preparation can also help reduce the levels of endogenous...

  8. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    ...pAAV-Ef1a-fDIO-GCaMP6s Neurotransmitter sensors: Dopamine and norepinephrine Neuroscience research relies on accurate monitoring...measurements of dopamine (113049, 113050) or norepinephrine (123308). The two dopamine sensors offer different...of our viral vectors blog posts Find tips for preparing AAVs Download our viral vectors 101 eBook Resources...

  9. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    ... development of several fluorescent autophagy reporters now allows for the measurement of autophagic flux... al. "Rosella: a fluorescent pH-biosensor for reporting vacuolar turnover of cytosol and organelles in...using a single fluorescent biosensor." Scientific reports 5 (2015): 12397. PubMed PMID: 26215030. PubMed ...

  12. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    ...egg-white protein avidin (or its derivatives streptavidin and neutravidin) is for all practical purposes...purification of biotinylated protein on blots with Streptavidin-phosphatase (Jackson ImmunoResearch, 016-050...Addgene.org Learn about Popular Plasmids in the Repository Learn about New Plasmid Technologies in our Hot...

  14. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

    Type
    Blog Post
    ...enhanced GFP (EGFP)-type termini. mNeonGreen was reported as the brightest monomeric green or yellow fluorescent...a protein which had a total of 31 amino acid replacements relative to CFP484. Because the excitation and... or for live cell imaging of fusions and FRET reporters. mTFP1 is also insensitive to physiologically ...

  15. Plasmid Cloning by PCR

    Type
    Blog Post
    ...amplify and design primers that will bind to and replicate it. The following image shows the ends of the ...of 5’-TGCTTAGCGGCCGCTCAGTACTTCGAGATATGCCA-3’. Preparing the PCR product for cloning Run the PCR reaction...your final plasmid by sanger sequencing. DNA replication by PCR has error rates that range from roughly...

  16. Another Pathway into Cells: iTOP

    Type
    Blog Post
    ... cells and activate expression of a luciferase reporter. CPP was not required for protein entry into cells...primary cell types using Cre and various loxP reporters. In both mouse and human embryonic stem cells,...Plasmids @Addgene: iTOP-tested proteins and reporters Previous Protein Delivery Techniques: Introduction...

  17. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type
    Blog Post
    ...flexible multidirectional control, a new study repurposes the sgRNA to include modular synthetic RNA scaffolds...single guide RNA in the CRISPRi system can be repurposed to include modular RNA scaffolds. These RNA...AdamChinFatt     References Qi, L. S. et al. Repurposing CRISPR as an RNA-guided platform for sequence-specific...

  18. The MTA at Addgene

    Type
    Blog Post
    ...with the number of requests and deposits in our repository. From 2004 to 2023, we facilitated 784,795 MTAs...MTAs per year.  The MTA Process at Addgene As a repository, Addgene doesn’t actually own the majority of...party (signatory) to the MTAs for plasmids in our repository. Instead, we facilitate the signing of MTAs by...

  19. Trading Spaces - Visiting Scientist Edition

    Type
    Blog Post
    ...if they have specialized skills and equipment! Preparing for your visit   Design and communicate a research... science usually doesn’t follow our timelines! Prepare a research plan to set yourself up for success ... bench mentor wait on you!), make sure you are prepared (read any protocols the day before, etc.), take...

  20. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    ... organisms formally classified as fungi. They reproduce by either fission or budding, which is the primary...joining (NHEJ) to perform DNA double strand break repair instead of homologous recombination (HR). HR is...important gene co‑expression modules. Biomedical Reports 7.2 (2017): 153-158. DOI: 10.3892/br.2017.941 Bernauer...
Showing: 581 - 600 of 957 results