We narrowed to 972 results for: REP

...types. Purpose  SpCas9 This plasmid expresses Streptococcus pyogenes (Sp) Cas9 from a Cbh promoter. Cbh ...un-tagged versions of SpCas9 and further variants replace SpCas9 with SpCas9n (nickase), which nicks DNA ...breaks and may better facilitate homology directed repair. You can find all of these variants, as well as...

...Urbana-Champaign. The PUF proteins include eight modular repeats, each of which binds a consecutive base in its ...colleagues led by Zhao described and demonstrated in a report published in The Journal of Biological Engineering...Engineering in March is fused to a translational repressor. In principle, however, designer PUF proteins can...

...plasmids” from last March were quite popular and represent the diversity of tools we share on behalf of scientists...and guest blogger Erik Snapp breaks down how to prepare a chalk talk for an academic job interview including... blog posts Resources at Addgene Browse the repository Find educational resources at Addgene Search ...

...deaminase (TadA) to a catalytically impaired Streptococcus pyogenes Cas9. They called this editor Target-ACE...ribonucleoproteins for transient expression. They replaced the tetraloop of the sgRNA scaffold with a com...adenosine-to-inosine (A-to-I) RNA editor called REPAIR by fusing catalytically inactivated RNA-targeting...

...majority of them remain uncharacterized. This represents a considerable barrier to furthering research...in Cancer.” Of all the mutations and fusions represented in this collection, approximately 80% (over 800...) and EGFR (Plasmid 116731: pHAGE-EGFR), are represented in this collection along with hundreds of less...

... created the IBEX Knowledge-Base, a community repository and resource for spatial biology enthusiasts....contributors, including 61 fluorescent probes, representing imaging from 47 different tissues. Researchers...Imaging Community’s vision is an open, global repository for reagents, protocols, panels, publications...

...conduct a two hour long interview. I anxiously prepared for the call. The length of this interview was...was to do for my time at Addgene. This training prepared me for the independent work on the QC team. I ...and Geneious, I was able to determine if any discrepancies between what we sequenced in house and the submitted...

...is the 21st century and all. But while the auto-reply telling you your message was received is a nice ... on hold, let alone waiting multiple days for a reply to an email. You need answers: the right ones, quickly...everyone involved. We’re adding self service to the repertoire of tools you can use to get support for our resources...

...for a successful application. It takes time to prepare an excellent resume…it is worth it. Read here to... to get help if you are having trouble preparing your most excellent resume. 2) Follow job boards and ...monitoring submissions (usually a Human Resources representative). Pro tip: if there is no place to upload a...

...SpyCatcher system, based on a protein isolated from Streptococcus pyogenes that locks itself together, to produce...they call SpyLigase (Spy comes from the “S” in Streptococcus and the “py” in pyogenes), capable of locking...links in the chain.  Addgene: What about the Streptococcus pyogenes protein domain made it ideal for this...

...accessible to scientific entrepreneurs. From emerging technologies to underrepresented research areas, we're...contributed to 9,000 publications in 2025 alone, each representing questions answered, hypotheses tested, and knowledge...

...much more forgiving and can be mostly or entirely replaced with DNA bases while retaining activity in both...specifically recognizes its folds, especially the repeat:antirepeat duplex. Cas12 recognizes the 2’-OH of RNA ...

...booth about amazing work already represented in the Addgene repository (like these new Rainbow Bacterial... we’ve curated microbiology plasmids from the repository onto one handy Microbiology Resource page and...

...CRISPR (clustered regularly interspaced palindromic repeats) Cas (CRISPR associated genes) system. This popular...coli in 1987 (1), the concept that these clustered repeat sequences operated as a safeguard against bacteriophages...for their ability to devise new strategies to reproduce within a host cell. A few virus particles have...

...pAAV-Ef1a-fDIO-GCaMP6s Neurotransmitter sensors: Dopamine and norepinephrine Neuroscience research relies on accurate monitoring...measurements of dopamine (113049, 113050) or norepinephrine (123308). The two dopamine sensors offer different...of our viral vectors blog posts Find tips for preparing AAVs Download our viral vectors 101 eBook Resources...

...enhanced GFP (EGFP)-type termini. mNeonGreen was reported as the brightest monomeric green or yellow fluorescent...a protein which had a total of 31 amino acid replacements relative to CFP484. Because the excitation and... or for live cell imaging of fusions and FRET reporters. mTFP1 is also insensitive to physiologically ...

...primary antibody that is directly conjugated to your reporter circumvents the issue. However, directly conjugated...which uses just a primary antibody that has your reporter (in this case a fluorophore) directly attached...biorender.com. Proper tissue processing and sample preparation can also help reduce the levels of endogenous...

... This allowed us to create pseudoviruses that represent lineages and variants that are actually transmitting... assay, the pseudoviruses express a luciferase reporter which is used as a readout for infection of ACE2...pseudovirus was similar to that of pseudoviruses representing a bat-derived coronavirus and SARS-CoV. These...

... development of several fluorescent autophagy reporters now allows for the measurement of autophagic flux... al. "Rosella: a fluorescent pH-biosensor for reporting vacuolar turnover of cytosol and organelles in...using a single fluorescent biosensor." Scientific reports 5 (2015): 12397. PubMed PMID: 26215030. PubMed ...

... plasmids in the repository, each verified by sequencing, which makes the repository a convenient source...most common expression system within the Addgene repository is mammalian, but the largest source of unique...