We narrowed to 641 results for: ato

...Response against the Tetracycline-Dependent Transactivator Correlates with Long-Term Doxycycline-Regulated...

... tries 5 separate experiments at once to get confirmatory data for a hypothesis and presents only the ...

...a hose to a nitrogen gas tank with a pressure regulator. Other methods for physical shearing exist as ...

..., setting up bases and contacts with Spanish laboratories to start working on projects together. List ...

...may not be the most accessible option for many laboratories. Additionally, it can be difficult to distinguish...

...to researchers.  TALENs In 2010, transcription activator-like effector nucleases (TALENs) were engineered...

...currently a research scientist in the Department of Laboratory Medicine at the University of Washington. She...

...it alone. The help of fellow lab members and collaborators will not only get your paper out the door much...

...bacterial colonies. Equipment Shaking incubator at 37 °C Stationary incubator at 37 °C Water bath at 42 °C Ice...temperature and then (optional) incubate in 37°C incubator. Mix 1 - 5 μl of DNA (usually 10 pg - 100 ng) ...antibiotic) to the bacteria and grow in 37°C shaking incubator for 45 min. Pro-Tip This outgrowth step allows... do this you will need to have access to an electroporator and the appropriate cuvettes. Follow the manufacturer's...

...Samai, P., Pyenson, N., Jiang, W., Goldberg, G. W., Hatoum-Aslan, A., & Marraffini, L. A. (2015). Co-transcriptional...

..."Photoactivatable genetically encoded calcium indicators for targeted neuronal imaging." Nature methods...

...Yellow-orange fluorescent proteins like TagRFP, tdTomato, DsRed, the mKate series, or tdKatushka2 (Drobizhev...

...lesional skin from a psoriasis patient, showing an inflammatory phenotype. Each protein was detected using an...

...personal protective equipment including laboratory coat, laboratory goggles, and gloves. Sodium azide containing...Class II, Type A2 Biological Safety Cabinet 4 °C Refrigerator Pipette controller Benchtop centrifuge compatible...tubes NanoDrop spectrophotometer 37 °C, 5% CO 2 incubator with shaking platform set to 120 rpm 37 °C bead...upright at -20 °C Procedure Section 1: Affinity chromatography Equilibrate filtered tissue culture supernatant...

...deposit, associate the plasmids with a Principal Investigator (PI), provide us with your shipping address ...

...conformational changes that make Cas9 such a valuable laboratory tool are still an area of active research. New...

...Harvest antibody Equipment Biosafety Cabinet 4 °C Refrigerator Micropipettes and tips Pipettor and pipettes...conical tubes Automated cell counter 37 °C, 5% CO 2 incubator with shaking platform set to 120 rpm 37 °C bead... mL vented flask.Incubate in a 37 °C, 5% CO 2 incubator on a shaking platform set to 120 rpm. Pro-Tip ...swirl 5–10 times to mix. Return the flask to the incubator. Section 3: BCD Feed and valproic acid supplementation...

...19246619.Downey N. Interpreting melt curves: An indicator, not a diagnosis. IDT, 2014. Dwight Z; Palais ...

...Konermann, S., Trevino, A. E., Scott, D. A., Inoue, A., Matoba, S., Zhang, Y., & Zhang, F. (2013). Double nicking...

..., Zalatan JG (2018) Synthetic CRISPR-Cas gene activators for transcriptional reprogramming in bacteria...