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We narrowed to 971 results for: lars

Showing: 581 - 600 of 971 results

  1. 2022 and 2023: Reflections and Looking Forward

    Type
    Blog Post
    ... countries. Guides and protocols were our most popular content, while our beloved blog received 1.3 million...scientists in this rapidly changing environment. In particular, we are working to understand what challenges...Addgene colleagues, collaborators, partners, and the larger scientific community to maximize the positive impact...

  2. Fluorescent Proteins 101: History of Fluorescent Proteins

    Type
    Blog Post
    ...like GFP came to be used as research tools is particularly interesting. Although humans have known about...crystal jellyfish Aequorea victoria (Figure 1) in molecular detail. On the underside of the “umbrella” of ...Soon, the number of people studying it grew. In particular, studies in the laboratory of UCSD biochemist...

  3. Your Top Requested Plasmid in 2016!

    Type
    Blog Post
    ...without controversy, and it remains to be seen how popular any one of them will become. One thing is for sure...was... pSpCas9(BB)-2A-Puro (PX459) V2.0       Similar to last year, the top requested plasmid from 2016...peptides. PX459 This is the vector backbone. This particular backbone can be used to transfect a variety of...

  5. Microbes: Look, Listen, and Tell

    Type
    Blog Post
    ...microscopes work as conventional microscopes and give a similar optical quality (magnification of 140X and 2 micron... to microbes by using non-invasive methods for cellular imaging with ultrasound. In his research he applies...applies naturally occurring bacterial “molecular buoyancy devices” (also known as gas vesicles) as acoustic...

  6. Designer PUF Proteins for Any RNA Target

    Type
    Blog Post
    ...Urbana-Champaign. The PUF proteins include eight modular repeats, each of which binds a consecutive base...on a version that she hopes will change the intracellular localization of RNA. “You can think of changing...the kit.” Reference Abil Z, Denard CA, Zhao H.  Modular assembly of designer PUF proteins for specific ...

  7. Pouring LB Agar Plates

    Type
    Protocol
    ... you will be screening a large number of colonies, we recommend using larger plates. Many labs use 100... plates with colored marker - particular colors correspond to particular antibiotics. Position the flame...good idea of what that volume looks like in your particular plate. For the remainder of the plates, pour ...

  8. Plasmid Cloning by PCR (with Protocols)

    Type
    Protocol
    ...for PCR Based Cloning The basic PCR primers for molecular cloning consist of: Leader Sequence: Extra base...TAAGCAGAATTCATGTGGCATATCTCGAAGTAC-3'. For the Reverse Primer, the design is similar, but we need to use the reverse complement to get... get PCR amplification. We can start similarly, taking the final 18bases of the ORF, including the stop...will need to use a phosphatase to prevent re-circularization of the vector. You should treat your digested...Additionally, if your final product is going to be very large (>10kb) you might want to use electro-competent ...

  9. Western Blot

    Type
    Protocol
    ...high molecular weight proteins and higher percentage acrylamide when immunoblotting low molecular weight...and reagents from other vendors should produce similar outcomes when using these protocols. However, please... ideal lysis buffer will vary depending on the cellular location of the protein of interest. RIPA buffer...buffer will vary depending on the sample type and cellular location of the protein of interest. You may need...

  10. A new platform for fast functional characterization of potentially cancerous mutations

    Type
    Blog Post
    ... responsible for cancer from genomics data, and large-scale patient cohort data from the MD Anderson Cancer...constructs using the High-Throughput Mutagenesis and Molecular Barcoding (HiTMMoB) technique (Tsang et al., 2016... perturbations in vitro using Ba/F3 and MCF10A cellular models. Ba/F3 and MCF10A are growth factor dependent...

  11. Phage Directory: From Phage Therapy to a Repository of Phage Information

    Type
    Blog Post
    ...correct phages will test. These includes labs with a large number of different phages or with phages targeting...that phage information was very fragmented. A particular phage researcher may only know how to prepare...networking Additional resources on Addgene.org Find molecular biology protocols here Visit the Addgene careers...

  12. CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]

    Type
    Blog Post
    ...production of deletions by paired DSBs may be particularly useful. The first is the study of non-coding...and Harvard Medical School. His lab studies the molecular genetics of blood cell development and disease...genomic deletion efficiency mediated by clustered regularly interspaced palindromic repeats (CRISPR)/Cas9 ...

  13. Who Gives a Tweet? 9 Facts About Scientists on Twitter

    Type
    Blog Post
    ...Instagram Social media and news sites were also popular. Perhaps scientists, like nonscientists, blur the...the more friends they have in common. Only the largest connected part of the follower network is shown...physical (blue) and social (orange)  scientists largely cluster by discipline, suggesting that scientists...

  14. IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging

    Type
    Blog Post
    ... Affinity Reagent Working Group of the Human Biomolecular Atlas Program (HuBMAP) antibody metadata standards.... Users can also recommend or not recommend a particular reagent in the given conditions, along with the...building both the community and the database. They particularly encourage people to upload and share their negative...

  15. 6 Steps to Submitting a Resume That Gets Seen

    Type
    Blog Post
    ...excellent resume. 2) Follow job boards and check them regularly Use your research skills to determine which job... to take next steps and follow up especially in large companies. It is fine to address the cover letter...might be able to help you. If the organization is large, you will need to be more directed and target someone...

  16. 9 Tips to Achieve Success in Academia

    Type
    Blog Post
    ...follow their passion. “There are many ways to have a large positive impact in society. We need much, much more...studies. They suggest that you make time for extracurricular activities both to develop new skills and to...information about the benefits of pursuing extracurricular activities. Success evolves from passion True...

  18. Tips for Improving Your Next Manuscript

    Type
    Blog Post
    ...welcoming us. Then it was time to work! Set up similarly to the online class, we met as a group for sessions...colleagues’ manuscripts and discuss changes to particular sections. The focus groups allowed us to try ...try out techniques that were discussed in the larger sessions and reinforced our newfound knowledge with...

  19. Networking and Inspiring Young Scientists at Freshers Fairs

    Type
    Blog Post
    ... events are one of the main ways budding young scholars across Europe get introduced to the cultures and...collaborations and conferences for their students. Another large goal of the organisation is to encourage iGEM alumni...these biological societies engaging with young scholars. We believe it’s very important to develop a network...

  20. Bringing Sustainable Practices to the Lab: Recycling

    Type
    Blog Post
    ...Anyone who works in a lab is familiar with the alarming amount of waste that is generated. This is a systemic...first post will be based on recycling! With the popularity of single-use items, plastic is a substantial... still be interested as it offsets the cost of regular waste services. Learn about how to start a glove...
Showing: 581 - 600 of 971 results