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...) is the movement of genetic material between organisms. It plays a key role in bacterial evolution and... blog posts on plasmid cloning Resources on Addgene.org Read our protocols for plasmid cloning Find ...

...favorite content collections is our Help Center, help.addgene.org, where you can easily search articles answering...reach out about writing a guest post (email news@addgene.org.) We are here to support and advance the scientific...

..., typically an expression construct, to cells/organisms. This is often cDNA, shRNA, or something similar...www.ncbi.nlm.nih.gov/books/NBK19423/ Resources on Addgene.org Addgene's most popular lentiviral plasmids Addgene's...

...analyte. This can be difficult to achieve in whole organisms, but AAVs make it possible. In AAV systems, different.... DOI: 10.3389/fncel.2019.00474 Resources on Addgene.org Fluorescent Proteins: Biosensors Viral Service...

...interest. This is a particularly nice feature as organ-specific injections, or the location of a specific...10):2073. DOI: 10.3390/v13102073 Resources on Addgene.org Adeno-associated Virus (AAV) Guide Addgene...

...basics.  Mesophiles vs. extremophiles Mesophilic organisms are your regular next-door microbes which grow...our synthetic biology blog posts Resources on Addgene.org Find plasmids for your synthetic biology experiment...

...A fundamental approach to studying membranes, organelles, and other subcellular compartments is via proteomics...makes PL a viable method for proteomics in model organisms in which it was previously not an option, including...

...transcription machinery differs between cell types or organisms, promoters are similarly variable. Bacterial promoters...posts from our Plasmids 101 series Resources on Addgene.org: Inducible Promoters for Bacteria Tetracycline...

...addition, there was an entire story-telling session organized by Story Collider and which featured a talk about...Neuroengineering Primer on Optogenetics Resources at Addgene.org Addgene's Viral Service Find Retrograde AAV ...

...are so ubiquitous in the lab that it is easy to forget that these enzymes naturally occur in bacteria ...Mutagenesis to Mutate Your Plasmids Resources on Addgene.org Check Out Our Plasmid Protocols Boost Your Molecular...

...available along with detailed protocols, go to www.addgene.org/crispr/tagging. Addgene: What motivated you... Kendall Morgan...

...processing machinery present in almost every living organism. Precursor, or pre-tRNAs are cleaved by RNase ... illustrated the power of polycistronic gene organization, and its translation to CRISPR/Cas9 shows great...

...activating any human gene, go to http://sam.genome-engineering.org/. Crystal structure of Cas9 in complex with... Kendall Morgan...

...helping researchers visualize the genome and its organization within the nucleus, also called the 4D nucleome...Control Protein Activity with GFP Resources on Asddgene.org Find Fluorescent Protein Plasmids Find Fluorescent...

...sample,” did the word convey the same concept to Morgan, our senior engineer? The answer to these questions...science communication blog posts Resources on Addgene.org Find careers at Addgene ...

...want to amplify sequences from a large genome organisms like mouse or human. There are several online ...about different plasmid elements Resources on Addgene.org Find protocols for plasmid cloning Read our ...

...Sequencing the 16S rRNA reveals the identity of the organisms present while sequencing of all transcripts gives...of our Plasmids 101 blog posts Resources on Addgene.org Check out our microbiology resource page Deposit...

...old-fashioned way: through physical outreach, visiting organizations, knocking on doors, and attending conferences...the globe.  “Read the Addgene guide: https://www.addgene.org/guides/crispr/” — SirHamhands, responding to...

...Introduction to Antibodies Additional resources on addgene.org  Antibody GuideRecombinant antibodies available...Format. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.M407888200 Kwon, N., Kim, Y., Lee, ...

... of your organization Time management Managing a team Leadership Resources on Addgene.org Check out...