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TypeBlog Post...basics. Mesophiles vs. extremophiles Mesophilic organisms are your regular next-door microbes which grow...our synthetic biology blog posts Resources on Addgene.org Find plasmids for your synthetic biology experiment...
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Plasmids 101: Inducible Promoters
TypeBlog Post...transcription machinery differs between cell types or organisms, promoters are similarly variable. Bacterial promoters...posts from our Plasmids 101 series Resources on Addgene.org: Inducible Promoters for Bacteria Tetracycline... -
A Novice's Perspective on Neuroscience - SfN2017
TypeBlog Post...addition, there was an entire story-telling session organized by Story Collider and which featured a talk about...Neuroengineering Primer on Optogenetics Resources at Addgene.org Addgene's Viral Service Find Retrograde AAV ... -
Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools
TypeBlog Post...A fundamental approach to studying membranes, organelles, and other subcellular compartments is via proteomics...makes PL a viable method for proteomics in model organisms in which it was previously not an option, including... -
How Addgene Creates Content
TypeBlog Post...favorite content collections is our Help Center, help.addgene.org, where you can easily search articles answering...reach out about writing a guest post (email news@addgene.org.) We are here to support and advance the scientific... -
Plasmids 101: Methylation and Restriction Enzymes
TypeBlog Post...are so ubiquitous in the lab that it is easy to forget that these enzymes naturally occur in bacteria ...Mutagenesis to Mutate Your Plasmids Resources on Addgene.org Check Out Our Plasmid Protocols Boost Your Molecular... -
Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers
TypeBlog Post...available along with detailed protocols, go to www.addgene.org/crispr/tagging. Addgene: What motivated you... Kendall Morgan... -
Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs
TypeBlog Post...processing machinery present in almost every living organism. Precursor, or pre-tRNAs are cleaved by RNase ... illustrated the power of polycistronic gene organization, and its translation to CRISPR/Cas9 shows great... -
Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens
TypeBlog Post...activating any human gene, go to http://sam.genome-engineering.org/. Crystal structure of Cas9 in complex with... Kendall Morgan... -
Addgene: The First Twenty Years
TypeBlog Post...old-fashioned way: through physical outreach, visiting organizations, knocking on doors, and attending conferences...the globe. “Read the Addgene guide: https://www.addgene.org/guides/crispr/” — SirHamhands, responding to... -
Viral Vectors 101: An Introduction to AAV
TypeBlog Post...types even when systemically administered in an organism. These features make AAV ideal for human therapies...Learn about Viral Vector Elements Resources on Addgene.org Read Our AAV Guide Find AAV Vectors Browse All... -
The time and cost required to make a plasmid
TypeBlog Post...where to find them! Keeping good records and an organized lab saves time and money across the board. Consider...Download the Plasmids 101 eBook Resources on Addgene.org Join the community of Addgene depositors here... -
CRISPRainbow and Genome Visualization
TypeBlog Post...helping researchers visualize the genome and its organization within the nucleus, also called the 4D nucleome...Control Protein Activity with GFP Resources on Asddgene.org Find Fluorescent Protein Plasmids Find Fluorescent... -
RaPID Detection of RNA-protein Interactions
TypeBlog Post...alternative option but it’s toxic to tissues and model organisms. Detecting RNA-protein interactions with RaPID...resources for studying chromatin Resources on Addgene.org Find Split-BioID plasmids Find CRISPR plasmids... -
Transferable Skills Guide: Problem Solving
TypeBlog Post... of your organization Time management Managing a team Leadership Resources on Addgene.org Check out... -
Transferable Skills Guide: Cross-team Communication
TypeBlog Post...sample,” did the word convey the same concept to Morgan, our senior engineer? The answer to these questions...science communication blog posts Resources on Addgene.org Find careers at Addgene ... -
Troubleshooting Your Plasmid Cloning Experiment
TypeBlog Post...want to amplify sequences from a large genome organisms like mouse or human. There are several online ...about different plasmid elements Resources on Addgene.org Find protocols for plasmid cloning Read our ... -
Plasmid Tools for Microbiome Studies
TypeBlog Post...Sequencing the 16S rRNA reveals the identity of the organisms present while sequencing of all transcripts gives...of our Plasmids 101 blog posts Resources on Addgene.org Check out our microbiology resource page Deposit... -
Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics
TypeBlog Post...been used to construct plasmids for use in model organisms such as E. coli and yeast. Because this approach...2019. This novel tool is practical in cell and organ culture, as well as cell lineage studies. Eisenhoffer... -
Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute
TypeBlog Post...government. All the Health and Human Services (HHS) organizations have so many [science communication] needs. ...Other Science Communication Posts Resources on Addgene.org Explore careers at Addgene Other Resources ...