We narrowed to 602 results for: she

1. Plasmids 101: Restriction Cloning

   Type

   Blog Post

   ...ratio” in the ligation reaction. 5. Purify the finished plasmid   Once it looks like your ligation has...

2. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

   Type

   Blog Post

   ...PAM (bold highlighted text) and cleavage sites (slashes) are shown. In this case, the green guide would...

3. Antibodies 101: Multiplex Immunofluorescence

   Type

   Blog Post

   ...the secondary antibody, it should get completely washed away and leave no signal. If you see lots of extra...

4. CRISPR 101: Epigenetics and Editing the Epigenome

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   Blog Post

   ...Originally published Feb 14, 2017 and updated Jun 24, 2020. Epigenetic modifications are an additional...

5. Viral Vectors 101: AAV Serotypes and Tissue Tropism

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   Blog Post

   ...unlock” and infect specific cells. This protein shell packages the vector’s genetic material, and variations...

6. Virus Protocol - Generating Stable Cell Lines

   Type

   Protocol

   ...deliver antibiotic resistance. This protocol was established using 293T cells but can be adapted to alternative...

7. Plasmid Cloning by PCR (with Protocols)

   Type

   Protocol

   ...amount of recipient plasmid to insert. Isolate the Finished Plasmid Finally, you will need to pick individual...

8. Quick Guide to All Things Lentivirus

   Type

   Blog Post

   ... generations of lentiviral vectors have been established so far, with safety increasing in each generation...

9. When Fidelity Matters: A frank discussion about ligase fidelity

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   Blog Post

   ...New England Biolabs® (NEB) researchers recently published a method for the high-throughput profiling of ...

10. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type

    Blog Post

    ...837597 Li J-F, Chung HS, Niu Y, Bush J, McCormack M, Sheen J (2013) Comprehensive Protein-Based Artificial ...

11. AAV ddPCR Titration

    Type

    Protocol

    ...dUTP, Bio-Rad,1863023 10% Pluronic F-68, Thermo Fisher, 24040032 Droplet generation oil, Bio-Rad, 1863005...

12. Custom CRISPR Screens & the Green Listed Software

    Type

    Blog Post

    ...like to use in your screen. Currently, eleven published libraries are embedded. These were kindly donated...

13. Antibodies 101: Flow Cytometry

    Type

    Blog Post

    ...first resuspended in a pressurized buffer called sheath fluid and transported through tubes or capillaries...

14. Antibodies 101: Introduction to Antibodies

    Type

    Blog Post

    ...out of the solution. Once the unbound protein is washed away, the protein of interest is dissociated from...

15. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type

    Blog Post

    ... tagging is scalable to 96-well format. Off-the-shelf CRISPR reagents for the knock-in experiment were...

16. Typing CRISPR Systems

    Type

    Blog Post

    ... Alyssa Shepard...

17. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type

    Blog Post

    ...the many primary research papers that have been published at this point.  In brief, some standard opsins...

18. Selecting Your Plasmid Purification Kit

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    Blog Post

    ...Endotoxin-free, transfection-grade, BAC Thermo Fisher Beads, column, plate Mini, midi, maxi, mega, giga...

19. A Needle in a Base-Stack: Cas9 Structural Biology

    Type

    Blog Post

    ...Nishimasu, H., Ran, F. A., Hsu, P. D., Konermann, S., Shehata, S. I., Dohmae, N., Ishitani, R., Zhang, F., & ...

20. CRISPR Library Amplification

    Type

    Protocol

    ...is expected to work. 50 mL Falcon Conical tubes (Fisher, 14-432-22) Reagent Preparation Prepare, sterilize...