We narrowed to 846 results for: tat
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TypeBlog Post...later optimized these first generation FLiPs by mutating Dronpa to lower its aggregation tendency, increase...graduate student in Thomas J. Mansell’s lab at Iowa State University where she is developing fluorescent protein-based...
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New and Upcoming Viral Vectors - June 2019
TypeBlog Post...high contrast between its resting state and its calcium-bound state, which was achieved by lowering the... -
FlipGFP, a novel fluorescence protease reporter to study apoptosis
TypeBlog Post...cellular components ultimately leading to cell fragmentation and death (Elmore, 2007). To study apoptosis...fluorescence based reporters however have several limitations. First, FRET signals are often difficult to detect... -
Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids
TypeBlog Post...reproducibility of experiments. Additionally, proper citation of these Addgene IDs in published work allows ... on the Addgene Blog Read a blog post on Our Citations Feature Check out this post on Scientific Reproducibility... -
Summer SciComm Series: A PhD in Science Communication
TypeBlog Post...makes it easier to spot even minor weakness or limitations in a piece of research (Drummond & Fischhoff,... the National Academy of Sciences of the United States of America, 114(36), 9587-9592. doi:10.1073/pnas... -
Plasmids 101: Gram Negative and Positive Bacteria
TypeBlog Post...Society for Microbiology (2005). https://asm.org/getattachment/5c95a063-326b-4b2f-98ce-001de9a5ece3/gram-stain-protocol... -
New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor
TypeBlog Post...However, the first two generations of iGluSnFR had limitations: they were not optimal for experiments requiring...photon imaging. Figure 1: 1P and 2P excitation and emission spectra of SF-Venus-iGluSnFR.A184V... -
Predicting Adverse Reactions to Monoclonal Antibody Drugs
TypeBlog Post...under CC BY from Mattei et.al., 2022. Limitations of the in silico approach The in silico approach... verification. All approaches have their own limitations. Using methods together can inform decisions ... -
The Power Behind NGS Plasmid Validation: seqWell
TypeBlog Post...below. Normalization is a fundamental and often understated problem in multiplex NGS library preparation....seqWell, Inc. He is a sequencing technologist and computational biologist with a passion for solving critical... -
How to Lead a Great Meeting
TypeBlog Post... the purpose of the meeting: Begin meetings by stating “The purpose of this meeting is to _________." ...ambassador” from each affected team to be the representative in the meeting and then communicate outcomes... -
Components of CRISPR/Cas9
TypeBlog Post...LbCpf1 RR variant 5' TYCV AsCpf1 RVR variant 5' TATV Campylobacter jejuni (CJ) 3' NNNNRYAC Neisseria... as a scaffold linking the crRNA to Cas9 and facilitates processing of mature crRNAs from pre-crRNAs derived... -
Synthetic Photobiology: Optogenetics for E. coli
TypeBlog Post... image, as well as a genetic method for the computational problem of edge detection. Tabor et al. subsequently...some promoter activity persisted in the inactive state. The dynamic range, defined as the difference between... -
Finding and Joining Your Dream Lab
TypeBlog Post...Potential graduate students typically go through a rotation, which reveals their working habits, personality...the PI on any paper. Or perhaps, the lab has a reputation for not entering collaborations. Alternatively... -
Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)
TypeBlog Post...SLIC compare to other cloning methods? SLIC’s limitations arise from its dependence on single-stranded ...may be assembled incorrectly. To overcome this limitation, one option is to perform a hierarchical assembly... -
Flow Cytometry Readouts: Yes, No, and Everything in Between
TypeBlog Post... are positive for your marker would be a good statistic to compare between samples. In cases where there...characterize your samples may allow for better interpretation. Priyamvada Prathima has a research background... -
Antibodies 101: Buffers, Storage, and Conjugates
TypeBlog Post... to use, not sensitive to light in its inactive state, and cheap. Unless there’s a specific reason you...a specific wavelength of light in response to excitation by a laser, which is then read by a detector ... -
Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering
TypeBlog Post...screens that looked for a CRISPR repeat array near a putative effector nuclease. The Cas13d proteins they identified...targeting the MAPT exon 10 splice acceptor and two putative splice enhancers. After AAV-mediated delivery ... -
A Deep Dive into BioBuilder
TypeBlog Post...BioBuilder curriculum is now taught in almost every state in the U.S. and in more than 30 countries around...The BioBuilder Learning Lab is a fully equipped, state-of-the-art lab embedded in LabCentral’s community... -
Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies
TypeBlog Post...weeks of waiting, your flies will be ready for experimentation. Drosophila are amenable to many different ...PMCID: PMC1526508. 2. H. Jiang, et al. Cytokine/Jak/Stat signaling mediates regeneration and homeostasis ... -
A Practical Approach to Choosing the B(right)est Fluorescent Protein
TypeBlog Post...determine brightness are the extent to which the excitation light is absorbed and the efficiency by which...account, including the specs of your microscope (excitation wavelength, available emission filters, and detector...