We narrowed to 404 results for: pho;
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TypeBlog Post...al disrupted interactions between Cas9 and the phophate backbone of the target DNA strand via mutations...
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Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP
TypeBlog Post...FPs optimized for oxidizing environments to photoconvertible variants, it seems like GFP is always learning... -
Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing
TypeBlog Post...Louisiana State University. She is a fan of photosynthesis and writes about autotrophs and biochemistry... -
Plasmids 101: Modular Cloning
TypeBlog Post...Level 1 TUs with unique promoters, and the ideal phosphatase (or any modifying enzyme) to pair with it can... -
Quickest Way to Deposit Plasmids: The Deposit Spreadsheet
TypeBlog Post... Addgene account username. Shipping address and phone number. Plasmid sequences or GenBank files. We encourage... -
Overcoming the Challenges of Lentiviral Production
TypeBlog Post...transfection method (i.e. polyethyleneimine, calcium phosphate or liposomal methods), the volume of the reaction... -
Popular Retroviral Vectors and Their Uses in Scientific Research
TypeBlog Post...genes via infection with a single ecotropic or amphotropic MuLE lentivirus provides significant genetic ... -
Adenoviral Vector Production and Troubleshooting
TypeBlog Post...looking at absorbance at 260nm using UV/Vis spectrophotometry. An absorbance of at 260nm corresponds to... -
CRISPR in the Clinic
TypeBlog Post...CRISPR spin. CRISPR CAR-T treatments for leukemia, lymphoma, and some solid tumors are currently in early-... -
Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene
TypeBlog Post...quantification of various intermediate steps, a sizing electrophoretic gel, and qPCR quantification of the final libraries...