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  1. Finding and Joining Your Dream Lab

    Type
    Blog Post
    ...professors how they hire scientists for their labs. Tom Ellis selects new hires from a pool of CVs collected from... awhile. Acknowledgments Thank you to Dr. Tom Ellis (Imperial College London), Dr. Connie Cepko (Harvard...

  2. Plasmids 101: Methylation and Restriction Enzymes

    Type
    Blog Post
    ...methylation should be considered when digesting DNA. Why? Well, even though Dam methylation sites are not specifically...later be verified by Sanger sequencing. How can I tell if my enzyme will cut? Whether performing a digest...

  3. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    ... facet of a project this big. Maintaining our excellent service and accuracy involves caring and careful... it took a lot of work and dedication to do this well ... because it did. So finally, a big thank you ...

  4. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    ...requirements in regard to their sequence (NGG, etc.) as well as their orientation relative to the gRNA (5’ or...References References Adler, B., Trinidad, M., Bellieny-Rabelo, D., et al. (2023). CasPEDIA Database: ...

  5. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    ...quantitatively evaluating antibody staining, or counting cells are now routine, open-source software projects that...portable device for studying plankton using flow cells, has become an established alternative to high-priced...

  6. Targeted Mutagenesis with EvolvR

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    Blog Post
    ...EvolvR could also be a tool for lineage tracing cells that do not tolerate double-strand DNA breaks by...researchers to trace the progeny of a single starting cell. Additionally, multiplexing of guides allows EvolvR...

  7. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    ... a journal club to my molecular biology class. A Cell paper. With six figures. Averaging ten panels each...strategy that can help. Even something as simple as telling yourself, “I can stop and check my notes if I need...

  8. Antibodies 101: Fab Fragments

    Type
    Blog Post
    ...are trying to block but your primary antibody as well.  Next, Fab and Fab’ fragments are almost interchangeable...fragments for multiplexed super-resolution microscopy. Cell Rep Methods 2(10):100301. https://doi.org/10.1016...

  9. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    ...Moreover, avoidance of ‘to be’ verbs creates strong, well-directed meaning and delivers strength to the sentence...convince the reviewers that the proposal is strong, well-thought out, and will be successful when funded....

  10. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    ...contained all the goldilocks antibodies in one place... well, lucky for you one is in the making by Dr. Zhuhao... Innovative Neurotechnologies (BRAIN) Initiative Cell Census Network, the Wu lab will be screening over...

  11. How to Prepare for an Industry Interview

    Type
    Blog Post
    ...for my interviews in industry. Prepare stories to tell in your interview The STAR interview method is a...prepare stories to answer interview questions. Story telling shares many of the same elements as a STAR answer...

  12. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    ...developed by the Allen Institute for Artificial Intelligence. Semantic Scholar analyzes publications and ...paper has is displayed at the top of the page, as well as the number of citations highly influenced by ...

  13. A Look at Addgene's QC Process

    Type
    Blog Post
    ... the deposit, NCBI sequence and protein data, as well as available backbone sequences. All of these resources..., with significant contributions from Alyssa Cecchetelli, Cary Valley, and Holly McQueary. Resources ...

  15. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    .... The computer scientists can get involved in modelling, the electronics folks can build a robot worm ...rich and complex microbiome data for analysis. And, well, cats... everyone knows the internet loves cats!...

  18. Common Injection Routes in Mice

    Type
    Blog Post
    ...different substances can be injected, including cells, drugs, DNA, viral vectors, and viruses. All researchers... injections are most often used when injecting a cell suspension to create xenograft models. Restraint...

  26. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    ...access, review, and query. Gates Open Research and Wellcome Open Research both accept deposit of data associated...with two of the five: Figshare and Dataverse, as well as the DSpace repository. These integrations utilize...

  27. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    ...contributed by guest blogger, Kristian Laursen from Cornell University. Site directed mutagenesis is a highly... Laursen! Kristian Laursen is a researcher at Cornell University.       References 1. Breslauer, Kenneth...

  28. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    ...notation. Please indicate the amino acid change as well as any functional consequences of that change. For...for a lentiviral vector to be used in mammalian cells, check Lentiviral and Mammalian Expression). This...

  29. 2022 Depositor Week

    Type
    Blog Post
    ...Hello and welcome to Depositor Week 2022! It’s that special time of year where we Addgenies use our social...
Showing: 901 - 930 of 1024 results