Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 1,104 results for: Ide

Showing: 901 - 930 of 1104 results

  1. Rabies and Neuronal Tracing

    Type
    Blog Post
    ...molecular tools to map neuronal connectivity are widely used in neuroscience. In this post, I’ll describe... in first order neurons, the G-deleted RABV can divide and replicate (since these processes are mediated...Another study used RABV monosynaptic tracing to identify inputs to two major dopaminergic cell populations...

  2. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    ...initiation step of gene transcription; today we'll provide an overview on how transcription stops, or termination...post-transcriptional additional of multiple adenine (A) nucleotides to the tail of a messenger RNA transcript. The...be detailed here, but additional references are provided at the end.  Nearly all common bacterial expression...

  3. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    ...SpCas9. Hu et al. performed GUIDE-seq analysis to characterize genome-wide off-targets, since increasing... Resources on Addgene.org Check out our CRISPR Guide Find CRISPR Plasmids for Mammalian Systems Learn...

  4. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    ...Even before fluorescent proteins (FPs) came into wide use, there were a variety of ways to monitor cell...few properties that have now become hallmarks of ideal FP timers: 1. Ratiometric determination of expression...Empty Backbones Check Out Our Fluorescent Protein Guide ...

  5. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    ...below. Types of Voltage Indicators GEVIs can be divided up structurally into 2 main groups: voltage sensitive...voltage sensing component (i.e., rhodopsin naturally resides in the membrane), or both. Targeting these systems...Addgene’s Virus Protocols Addgene’s Chemogenetics Guide Resources on the Addgene blog Viral Vectors ...

  6. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    ...that popularity through the years.  Libraries are divided into different categories based on function: knockout... years, even as more CRISPR technologies become widely used and available. If we look closely at Figure...navigate that learning curve. As of 2023, our CRISPR guide has been viewed 1,904,462 times, and the CRISPR ...

  7. Plasmids 101: Degron Tags

    Type
    Blog Post
    ...Degrons are destabilizing domains which will be identified by various enzymes and sent to the proteasome...protein for imaging. Given its popularity and widespread usage, a proteolysis-targeting chimera (PROTAC...some stage, then choosing a HaloTag could pay dividends down the road. It is difficult to predict precisely...

  9. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    ...factor of 2. This means that if you set up two identical assays with the same sample, you could end up ... should fall within the range of the assay for a wide range of titers. We usually dilute our samples 1...experiments.  References Furuta-Hanawa, Birei, Teruhide Yamaguchi, and Eriko Uchida. "2D droplet digital...

  10. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    ... codon, stop codons, reading frame considerations, etc). This video demonstrates how to use the Snapgene...Protocols Watch Our Diagnostic Restriction Digest Video Check Out Our Molecular Biology Reference Pages  ...

  11. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    ...sites. FRT sites and loxP sites differ at the nucleotide level but share an overall structure of 13 base...37 °C). Smart molecular evolution led to the identification of FLPe which has a temperature optimum of ...Recombination between the two target sites with identical sequence leads to inversion of the flanked DNA...

  12. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    ...more funding and research opportunities are being provided to immunotherapy than ever before. Although different...from many other types of cancer. Mesothelioma is considered an orphan cancer; it is rare disease with only...(ADAO) are working toward such a ban, and the president of the organization, Linda Reinstein – whose husband...

  13. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

    Type
    Blog Post
    ...fluorescence can occur. Cre recombinase can be used alongside common, existing tools for fly research that use...turnover of Langerhans cells in the dermis and identify that Langerhans cells are replenished through ...The intestinal stem cell markers Bmi1 and Lgr5 identify two functionally distinct populations." Proceedings...

  14. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    ...results in a polymerase error rate of up to 2% per nucleotide position. The “sloppy” PCR reaction is created...ssDNA is packaged into phage and then extracted. Besides taking a couple days to generate the phage-derived...strand: A mix of degenerate primers and the high-fidelity Phusion polymerase are used to introduce point...

  16. Addgene Launches Partnership with IPI

    Type
    Blog Post
    ...Innovation (IPI). Together, we’ll be working to provide researchers with a collection of IPI’s well-validated...on our website and blog, including our antibody guide, antibody protocols, and Antibodies 101 blog series...

  17. Introducing...Addgene Quick Tips!

    Type
    Blog Post
    ...      And you’ll be able to find all published videos in our Quick Tips playlist:  Want your quick tips...be sharing our favorite parts of every quick tip video on our TikTok! Be sure to subscribe to our YouTube...

  18. Help Us Name Blugene’s New Virus Friend

    Type
    Blog Post
    ...your favorite name. We hope you submit your best ideas because the finalists will receive some newly designed...Learn more about Blugene's make-over Get a how-to guide on the new Addgene search engine ...

  20. We’re Going Viral This Week!

    Type
    Blog Post
    ...Learn more about the Data Hub in this blog post. Alongside our efforts to facilitate the sharing of reagents...Learn more about the viral service Read our AAV guide ...

  21. Lucky Thirteen: Guest Contributors of 2023

    Type
    Blog Post
    ..., tools, and knowledge. Guest authors The Codon Guide to Synthetic BiologyAuthor: Niko McCartyAntibodies...globe find and share knowledge, resources, and new ideas - we're so grateful for your writing, your knowledge...

  23. Twenty Years of Sharing Science!

    Type
    Blog Post
    ...experience we're sharing through blogs, eBooks, videos, guides, protocols, and of course, the friendly folks...

  25. Twelve Amazing Guest Blog Contributors!

    Type
    Blog Post
    ... from IPI Simon Moe, from Promega, who wrote a guide to selecting fluorescent dyes and ligands. Illuminating...Controls Priyamvada Prathima, who wrote a helpful guide to understanding your flow data. Flow Cytometry...

  26. Celebrating the 2024 Nobel Prize in Chemistry

    Type
    Blog Post
    ...awarded the Nobel Prize in Chemistry last week alongside Demis Hassabis and John Jumper! The Baker Lab ...like high resolution Ras sensors and crosslinked peptides proposed as a scaffold for a new generation of...

  27. Antibody Data Repositories and Search Engines

    Type
    Blog Post
    ...share validation and experimental data to help you decide if the antibody is a good fit for your experiment...improving antibody quality throughout research, consider checking out the Only Good Antibodies community...

  28. PEI Calculator for Planning AAV Packaging Transfections

    Type
    Blog Post
    ...you plan PEI transfections for AAV packaging, considering using this PEI Calculator. AAV packaging typically...Additional Resources on the Addgene Blog Important Considerations When Using AAVs AAVs Are Great Tools for Gene...

  29. New Podcast Segment: Hot Plasmids

    Type
    Blog Post
    ...We’re breaking into more audio and video on the Addgene Blog and Addgene website. As we push forward ... a Graduate Lab and Project Check Out 5 Science Videos from Tom McFadden Resources at Addgene.org Sign...

  30. New Viral Vectors - Spring 2025

    Type
    Blog Post
    ...the diverse needs of our community and support a wide range of experimental designs. Here are nine great... vectors? Browse our in-stock viral vectors or consider our AAV Packaged on Request service, which offers...
Showing: 901 - 930 of 1104 results